July

25^th-26^th We finalized our team members and elected the team leader, design the team name, logo, uniforms, and perimeters. We conducted laboratory safety training and brief schedule analysis.

28^th We made culture medium and buffer A and B, and done the PCR amplification.

29^th We did the plasmid conversion, spread bacterial colony, and shook Escherichia coli.

30^th We collected the linear no-load vector, which was detected by gel electrophoresis and recovered by gel extraction kit.

31^st We cultured and then centrifuged E. coli, to obtain the plasmid for further preservation.

August

1^st We did the restriction enzyme digestion, prepared the gel for the gel electrophoresis, transformed the plasmid, and poured the plates.

2^nd We did the inoculation, prepared LB culture media, and the protein induction.

3^rd We purified the protein through Ni-chelating affinity chromatography.

19^th-27^th We conducted the function test to analyze the protein activity.