Difference between revisions of "Team:Shanghai HS United/Notebook"

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{{IGEM_TopBar}}
 
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{{Shanghai_HS_United}}
 
 
<html>
 
<html>
  
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<div class="column full_size">
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<link rel="stylesheet" type="text/css"
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      href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_HS_United/StyleCSS&action=raw&ctype=text/css"/>
  
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<h1>Notebook</h1>
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<div class="flavone-lab-content">
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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    <!--navbar-->
 +
    <div class="navbar">
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        <a class="logo" href="https://2021.igem.org/Team:Shanghai_HS_United">
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            <img src="https://static.igem.org/mediawiki/2021/c/c1/T--Shanghai_HS_United--logo.png" alt="">
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        </a>
  
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</div>
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        <!--nav-->
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<div class="clear"></div>
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        <ul class="list">
 +
            <!--Team-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Team">Team</a>
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                <div class="child-nav">
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                    <ul class="child-list">
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                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Team">Team Members</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Attributions">Attributions</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Collaborations">Collaboration</a>
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                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
 +
            <!--Modeling-->
 +
            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Model">Modeling</a>
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            </li>
  
 +
            <!--Entrepreneurship-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Entrepreneurship">Entrepreneurship</a>
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            </li>
  
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<div class="column two_thirds_size">
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            <!--Implementation-->
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<h3>What should this page have?</h3>
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            <li class="item">
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<ul>
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                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Implementation">Implementation</a>
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<li>Chronological notes of what your team is doing.</li>
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            </li>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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</div>
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            <!--Human Practice-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Human_Practices">Human Practice</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Human_Practices">Integrated Human
 +
                                Practice</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Communication">Communication</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Fundraising">Fundraising</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<div class="column third_size">
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            <!--Parts-->
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<div class="highlight decoration_A_full">
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            <li class="item">
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<h3>Inspiration</h3>
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                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Parts">Parts</a>
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<p>You can see what others teams have done to organize their notes:</p>
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                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Parts">Parts
 +
                                Collection</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Engineering">Engineering</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<ul>  
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            <!--Project-->
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<li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li>
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            <li class="item active">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United/Description">Project</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Description">Description</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Experiments">Experiments</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Results">Results</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Proof_Of_Concept">Proof of Concept</a>
 +
                        </li>
 +
                        <li class="child-item active">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Notebook">Notebook</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_HS_United/Safety">Safety</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<li><a href="https://2019.igem.org/Team:Georgia_State/Notebook">2019 Georgia State</a></li>
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            <!--Home-->
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<li><a href="https://2019.igem.org/Team:Newcastle/Notebook">2019 Newcastle</a></li>
+
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_HS_United">Home</a>
 +
            </li>
 +
        </ul>
 +
    </div>
  
 +
    <!--图片-->
 +
    <div class="page-header">
 +
        <img src="https://static.igem.org/mediawiki/2021/9/97/T--Shanghai_HS_United--bg_1.png" alt="">
 +
        <h1>Notebook</h1>
 +
    </div>
  
−
<li><a href="https://2020.igem.org/Team:IISER-Pune-India/Notebook">2020 IISER Pune India</a></li>
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    <!--内容-->
−
<li><a href="https://2020.igem.org/Team:Lund/Notebook">2020 Lund</a></li>
+
    <div class="content-small">
−
<li><a href="https://2020.igem.org/Team:NOVA_LxPortugal/Notebook">2020 NOVA LxPortugal</a></li>
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        <section class="article p-t-72 p-b-54">
−
<li><a href="https://2020.igem.org/Team:RDFZ-China/NoteBook">2020 RDFZ China</a></li>
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            <img src="https://static.igem.org/mediawiki/2021/d/de/T--Shanghai_HS_United--img_notebook_1.jpg" alt="" style="width: 70%">
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</ul>
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−
</div>
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−
</div>
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 +
            <section>
 +
                <h1 class="title blue bold">July</h1>
 +
                <p><b>25<sup>th</sup>-26<sup>th</sup></b> We finalized our team members and elected the team leader, design
 +
                    the team name, logo, uniforms, and perimeters. We conducted laboratory safety training and brief
 +
                    schedule analysis.</p>
 +
                <p><b>28<sup>th</sup></b> We made culture medium and buffer A and B, and done the PCR amplification.</p>
 +
                <p><b>29<sup>th</sup></b> We did the plasmid conversion, spread bacterial colony, and shook Escherichia
 +
                    coli.</p>
 +
                <p><b>30<sup>th</sup></b> We collected the linear no-load vector, which was detected by gel electrophoresis
 +
                    and recovered by gel extraction kit.</p>
 +
                <p><b>31<sup>st</sup></b> We cultured and then centrifuged E. coli, to obtain the plasmid for further
 +
                    preservation.</p>
 +
            </section>
 +
 +
            <section>
 +
                <h1 class="title blue bold">August</h1>
 +
                <p><b>1<sup>st</sup></b> We did the restriction enzyme digestion, prepared the gel for the gel
 +
                    electrophoresis, transformed the plasmid, and poured the plates.</p>
 +
                <p><b>2<sup>nd</sup></b> We did the inoculation, prepared LB culture media, and the protein induction.</p>
 +
                <p><b>3<sup>rd</sup></b> We purified the protein through Ni-chelating affinity chromatography.</p>
 +
                <p><b>19<sup>th</sup>-27<sup>th</sup></b> We conducted the function test to analyze the protein activity.
 +
                </p>
 +
            </section>
 +
 +
            <br>
 +
            <br>
 +
            <img src="https://static.igem.org/mediawiki/2021/0/00/T--Shanghai_HS_United--img_notebook_2.jpg" alt="" style="width: 60%">
 +
        </section>
 +
    </div>
 +
 +
    <!--Footer-->
 +
    <div class="footer">
 +
        <div class="content">
 +
            <hr>
 +
            <span>Email: xiaowenxuan0526@gmail.com; caroline.niujl@gmail.com</span>
 +
            <span>Wechat Official Account: FlavoneLabRouse</span>
 +
        </div>
 +
    </div>
 +
</div>
 
</html>
 
</html>

Latest revision as of 13:49, 11 October 2021

Notebook

July

25th-26th We finalized our team members and elected the team leader, design the team name, logo, uniforms, and perimeters. We conducted laboratory safety training and brief schedule analysis.

28th We made culture medium and buffer A and B, and done the PCR amplification.

29th We did the plasmid conversion, spread bacterial colony, and shook Escherichia coli.

30th We collected the linear no-load vector, which was detected by gel electrophoresis and recovered by gel extraction kit.

31st We cultured and then centrifuged E. coli, to obtain the plasmid for further preservation.

August

1st We did the restriction enzyme digestion, prepared the gel for the gel electrophoresis, transformed the plasmid, and poured the plates.

2nd We did the inoculation, prepared LB culture media, and the protein induction.

3rd We purified the protein through Ni-chelating affinity chromatography.

19th-27th We conducted the function test to analyze the protein activity.



Email: xiaowenxuan0526@gmail.com; caroline.niujl@gmail.com Wechat Official Account: FlavoneLabRouse