Team:Worldshaper-Shanghai/Notebook

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Notebook

2021 Timeline

March:

· Team Gathering

· Professional theory training (online): molecular biology, scientific research, media management

April:

· Creating specific groups

· Professional theory training (online): synthetic biology, lab techniques

· Developing topic: focus on Alzheimer’s diseases and using full-length APP to create a drug screening model on drosophila

May:

· Professional theory training: bio-engineering

· iGEM competition rules learning

· project background research

Since June, members were divided into wet team and dry team. Everyone was arranged by different jobs.

June:

Wet Team:

· Getting ready for lab

· Researching relevant topic

Dry Team:

· Initiate a round of fundraising

· Developing online platform

· Start to construct wiki framework

· Planning of publicity propaganda

· Design team logo

· Designing team jersey

July:

Wet team:

· Start Lab:

oConstrcut APP plasmid

oDrosophila Crossing

oDrosophila behavioral experiment

· Writing daily Lab notes

Dry team:

· Interview planning

· Live media platform

· Start PR and raise attention of the project

· Printing, Distributing team jersey

· Initiate a new round of fundraising

· Planning on publicity events (School, Speech…)

August

Wet Team:

· In lab experiment

oDrosophila behavioral experiment

oDrosophila drug screening experiment

· Writing Lab Design and protocol

· Analyzing Data, ready for presentation

Dry Team:

· Modify partnership line

· Wiki design

· Publicity events

· Social research and interview with professionals of the field

· Researching and writing implementation.

September & October:

Wet Team:

· Finish Lab write up, design, notebook, protocol

· Uploads all wet team information on wiki

Dry Team:

· Researching and writing implementation

· Manuscript collection of human practice

· Finishing Wiki

Presentation video

Summer Journal

2021/0705

Lab crew uses PCR to replicate and create high number of APP 696 genes for further usage

2021/0706

Lab crew performs DNA gel electrophoresis to test for PCR results. DNA was then extracted, and performed Double Digestion using restriction enzyme to cut both APP gene and plasmid for connection. The lab crew left DNA and plasmid together overnight to create recombinant DNA

2021/0707

Lab crew transferred recombinant plasmid into competent E.coli cell. E.Coli was left overnight to reproduce so plenty of Recombinant plasmid was acquired. Then, E. Coli was sent to institution to transfer recombinant plasmid to Drosophila Chromosome.

2021/0708

Western blot
Crossed Drosophila to acquire genotype needed for experiment

2021/0712

Lab crew made culture medium for Drosophila in different concentrations of drug

2021/0713-14

Performed behavioral test: Mating Selection between young and old drosophila

2021/0715

Performed behavioral test: Female and male distinguishing courtship selection

2021/0721

Performed behavioral test: Pupa Height Test

2021/0722

Performed behavioral test: Adult locomotor test

2021/0728

Performed behavioral test: Drosophila learning and memory experiment

2021/0801

Performed behavioral test: Wing expansion defect test

2021/0803

Performed Larva eye disc and VNC disc anatomy with AO and Lysosome dye

2021/0805

Analyzed data from Larva anatomy experiment

2021/0807

Tested Effects of drugs on Drosophila growth

2021/0809

Tested effects of drugs on Drosophila lifespan