Hello, Welcome to SOFTWARE PAGE
Software Development Background
In the actual control experiment of each group, the relationship between
fluorescence intensity and time is different even if the template with
the same concentration is in each group of reaction because the reaction
start time is different from the measurement start time in the
experiment and the instrument is also affected by uncertain factors.
However, according to the experimental image, it can be found that the
error influence mentioned above does not change the trend of the
experimental image, but only changes the size of the specific value in
the image. Therefore, our team redesigned the experimental scheme
according to this experimental phenomenon, and designed a software to
correct the changes in experimental images caused by such errors.
Software Instructions
1. When opening this
program, users can see the initial interface as shown behind.
2. In the “Number of the
uncorrected curves” line, enter the number of curves(0~4). These curves
are from the same set of experiments and are used as standard curves in
the correcting function.
3. In the "Number of the
corrected curves" line, enter the number of corrected curves (0 ~
4).These curves are from the same set of experiments and are used as the
curves to be corrected in the correcting function.
4. After entering the
numbers, click "OK" button to enter the next interface
5. The interface is divided
into three parts.
6. Part 1 is the uncorrected
data input box, which is divided into CURVE1, CURVE2...Line. The number
of lines here is the value entered in the first line of the previous
interface.The first line in the data input box here serves as the
correcting curve data location.The first input box inputs the number of
columns of the file data (data read only supports in a row). The second
input box inputs the line number of the first data. The third input box
inputs the line number of the last data.The fourth input box inputs the
interval between data. The fifth input box inputs the label of the
curve.The following input boxes all enter data in this way.
7. Part 2 is the corrected
data input box, which is divided into CURVE1, CURVE2...Line. The number
of lines here is the value entered in the first line of the previous
interface.The first line in the data input box here serves as the
correcting curve data location(The first row of the two parts in the
experiment requires data from the experimental group at the same
concentration).
8. Part 3 has four
buttons.Button 1 is named “THE UNCORRECTED FILE”.You can select a file
with the XLS suffix for storing data by pushing this button. The data in
this file is selected as the data that will not be corrected in the
correcting function.
9. Button 2 is named
“PLOT”.You can graph the input data by pushing this button. This
function only provides drawing function and does not provide correction
function.
10. Button 3 is named
“Correct”. You can correcting the data and then graph the correcting
data by pushing this button.This function provides correcting function.
11. Button 4 is named “THE
CORRECTED FILE”. You can select a file with the XLS suffix for storing
data by pushing this button. The data in this file is selected as the
data that will be corrected in the correcting function.
12. You can get something
like the image beneath by pushing the "Plot" or "Correct" button after
you have entered the data.The function key marked with red circle on the
left can be used to enlarge the image, and the function marked with red
circle on the right can be used to save the image.
Attention
1. In order to improve the operation
speed of the correction function, the software limits the correction curve and only
supports the contractile correction of the corrected curve, that is, left and down
correction.
2. This software only supports reading
files with the suffix XLS.
3. This software is designed to address the current experimental results. In fact there are some alternatives, such as adding standard sample sets to the same batch before each measurement is performed and plotting the standard curve. But this method is obviously not convenient enough, so we propose the design of the software.
4. In the future, we plan to combine software and hardware to input current signals, convert them into curves, output and store target protein concentrations. We will also make the graphical interface more aesthetically pleasing and easier to use for users.