Team:UM Macau/parts

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Part Collection

Basic Parts

Click Part Number to link to the Part Page

Part Number	Name	Type	Description	Length(bp)
BBa_K3789000	SOD1	Regulator	SOD1 gene is a gene widely present in many organisms, which encodes superoxide dismutase 1 enzyme. It is a kind of antioxidant metal enzyme that plays an important role in the balance between oxidation and antioxidant. Oxidative stress occurs when there is an imbalance between the harmful reactive oxygen species (ROS) and antioxidants in the organism. During fermentation, oxidative stress causes damage to the yeast cell components. This can lead to yeast autolysis and purine is released as a result. Moreover, both leaking of the undesirable compounds during yeast autolysis and the oxidation reaction of the molecules in beer that are involved in beer flavoring can affect the flavor stability. SOD1 removes ROS by converting the harmful ROS to molecular oxygen and hydrogen peroxide. We use SOD1 in our project to improve the beer's antioxidant capability.	459
BBa_K3789001	chit42	Coding	The endochitinase enzyme encoded by chit42 can be utilized to produce chitooligosaccharide (COS), which is an antioxidant. Endochitinase breaks down the yeasts’ cell wall, in which the chitin abounds and produces COS. COS will serves as versatile functional chemicals possessing antimicrobial and antioxidant activities in beer. Finally, the content of three major staling compounds: 5-Hydroxymethylfurfural (5-HMF), phenylacetaldehyde (PAD), trans-2-nonenal (T2N) will be decreased.	1266
BBa_K3789002	PNP1	Coding	PNP1 is a gene that codes for a protein called Purine nucleoside phosphorylase. Purine nucleoside phosphorylase is an enzyme involved in purine metabolism, which can cleave a nucleoside by phosphorylating the ribose. Some research suggests that beer is especially bad for gout because it contains higher levels of purines that break down directly into uric acid. Introducing PNP into yeast cells can break down the free nucleotides in beer into purines and improve the utilization of purine. Finally, our beer can reduce the probability of gout. Source:The sequence we used comes from Saccharomyces cerevisiae S288c.	930
BBa_K3789003	TPS1 promoter	Regulatory	TPS1 promoter can promote the coding gene expression efficiently with ethanol exits. TPS1 is efficient in high-temperature conditions which always happens in the industry. The experiments show it enhanced as ethanol concentration increased from 3% to 8%.	1000
BBa_K3789004	pGK1 promoter	Regulatory	The pGK11 promoter regulates the 3-phosphoglycerate kinase (PGK1) gene in Saccharomyces cerevisiae. This promoter can efficiently drive high levels of expression of reporter genes.	1000
BBa_K3789005	Mating factor alpha prepro-leader (MF alpha)	Coding	The Mating factor alpha prepro-leader (MF alpha) is a widely used secretion signal leader which has been discovered from the Saccharomyces cerevisiae. This factor binds with copper(II) ions. It can fuse with the coding sequence of the recombinant protein and let the protein be secreted to the extracellular. This factor will induce when working with the mating-type yeast cell.	267
BBa_K3789006	ADH1 terminator	Terminator	A His-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.	188
BBa_K3789007	HA tag	Tag	human influenza hemagglutinin(HA) is a surface glycoprotein protruding out of the surface of human influenza virus and contributing to infectivity. It has been extensively used as an epitope tag when detecting the transformed exogenous vectors.	90
BBa_K3789008	pFA6a	Plasmid_Backbone	pFA6a is a precursor to plasmids carrying kanMX and other selector modules( HA tag, ADH1 terminator). And this plasmid is a commonly used backbone plasmid for generating epitope tagging vectors. The most common vector type is yeast genomic targeting used for saccharomyces cerevisiae.	4276
BBa_K3789009	6 x glycine	Coding	By inserting six glycines between two protein-encoding genes, the proteins can be prevented from interfering with each other, and they can fold normally to form the correct spatial structure.	18