Team:UM Macau/parts


Part Collection

Basic Parts

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Part Number Name Type Description Length(bp)
BBa_K3789000 SOD1 Regulator SOD1 gene is a gene widely present in many organisms, which encodes superoxide dismutase 1 enzyme. It is a kind of antioxidant metal enzyme that plays an important role in the balance between oxidation and antioxidant. Oxidative stress occurs when there is an imbalance between the harmful reactive oxygen species (ROS) and antioxidants in the organism. During fermentation, oxidative stress causes damage to the yeast cell components. This can lead to yeast autolysis and purine is released as a result. Moreover, both leaking of the undesirable compounds during yeast autolysis and the oxidation reaction of the molecules in beer that are involved in beer flavoring can affect the flavor stability. SOD1 removes ROS by converting the harmful ROS to molecular oxygen and hydrogen peroxide. We use SOD1 in our project to improve the beer's antioxidant capability. 459
BBa_K3789001 chit42 Coding The endochitinase enzyme encoded by chit42 can be utilized to produce chitooligosaccharide (COS), which is an antioxidant. Endochitinase breaks down the yeasts’ cell wall, in which the chitin abounds and produces COS. COS will serves as versatile functional chemicals possessing antimicrobial and antioxidant activities in beer. Finally, the content of three major staling compounds: 5-Hydroxymethylfurfural (5-HMF), phenylacetaldehyde (PAD), trans-2-nonenal (T2N) will be decreased. 1266
BBa_K3789002 PNP1 Coding PNP1 is a gene that codes for a protein called Purine nucleoside phosphorylase. Purine nucleoside phosphorylase is an enzyme involved in purine metabolism, which can cleave a nucleoside by phosphorylating the ribose. Some research suggests that beer is especially bad for gout because it contains higher levels of purines that break down directly into uric acid. Introducing PNP into yeast cells can break down the free nucleotides in beer into purines and improve the utilization of purine. Finally, our beer can reduce the probability of gout. Source:The sequence we used comes from Saccharomyces cerevisiae S288c. 930
BBa_K3789003 TPS1 promoter Regulatory TPS1 promoter can promote the coding gene expression efficiently with ethanol exits. TPS1 is efficient in high-temperature conditions which always happens in the industry. The experiments show it enhanced as ethanol concentration increased from 3% to 8%. 1000
BBa_K3789004 pGK1 promoter Regulatory The pGK11 promoter regulates the 3-phosphoglycerate kinase (PGK1) gene in Saccharomyces cerevisiae. This promoter can efficiently drive high levels of expression of reporter genes. 1000
BBa_K3789005 Mating factor alpha prepro-leader (MF alpha) Coding The Mating factor alpha prepro-leader (MF alpha) is a widely used secretion signal leader which has been discovered from the Saccharomyces cerevisiae. This factor binds with copper(II) ions. It can fuse with the coding sequence of the recombinant protein and let the protein be secreted to the extracellular. This factor will induce when working with the mating-type yeast cell. 267
BBa_K3789006 ADH1 terminator Terminator A His-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. 188
BBa_K3789007 HA tag Tag human influenza hemagglutinin(HA) is a surface glycoprotein protruding out of the surface of human influenza virus and contributing to infectivity. It has been extensively used as an epitope tag when detecting the transformed exogenous vectors. 90
BBa_K3789008 pFA6a Plasmid_Backbone pFA6a is a precursor to plasmids carrying kanMX and other selector modules( HA tag, ADH1 terminator). And this plasmid is a commonly used backbone plasmid for generating epitope tagging vectors. The most common vector type is yeast genomic targeting used for saccharomyces cerevisiae. 4276
BBa_K3789009 6 x glycine Coding By inserting six glycines between two protein-encoding genes, the proteins can be prevented from interfering with each other, and they can fold normally to form the correct spatial structure. 18

Composite Parts

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Part Number Name Component Parts Description Length(bp)
BBa_K3789010 PNP enzyme secretion system BBa_K3789004, BBa_K3789005, BBa_K3789009, BBa_K3789002, BBa_K3789007, BBa_K3789006 This composite part consists of pGK1 promoter, Mating factor alpha prepro-leader (MF alpha), 6 x glycine, PNP1 coding sequence, and ADH1 terminator. The product of this system is a secretable purine nucleoside phosphorylase(PNP) together with a HA tag. MF alpha enables the enzyme to be secreted. The six glycines between MF alpha and PNP1 coding sequence avoid the interruption between them, enabling them to form correct spatial structure. 2493
BBa_K3789011 SOD enzyme secretion system BBa_K3789004, BBa_K3789005, BBa_K3789009, BBa_K3789000, BBa_K3789007, BBa_K3789006 This composite part consists of pGK1 promoter, Mating factor alpha prepro-leader (MF alpha), 6 x glycine, SOD1 coding sequence, and ADH1 terminator. The product of this system is a secretable superoxide dismutase 1 enzyme(SOD) together with a HA tag. MF alpha enables the enzyme to be secreted. The 6 glycines between MF alpha and SOD1 coding sequence avoid the interruption between them, enabling them to form correct spatial structure. 2022
BBa_K3789012 Endochitinase enzyme secretion system BBa_K3789003, BBa_K3789005, BBa_K3789009, BBa_K3789001, BBa_K3789007, BBa_K3789006 This composite part consists of TPS1 promoter, Mating factor alpha prepro-leader (MF alpha), 6 x glycine, chit42 coding sequence, and ADH1 terminator. The product of this system is a secretable endochitinase together with a HA tag. MF alpha enables the enzyme to be secreted. The 6 glycines between MF alpha and chit42 coding sequence avoid the interruption between them, enabling them to form correct spatial structure. 2829




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