Team:UM Macau/Attributions

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Attribution

Team Students Principal Investigators FHS Staff Special Acknowledgement

Captain

Emma , MEI YINGYI

  • Team Affairs
    • Assign work to each group leader and coordinate the cooperation between groups. Organize regular meetings between team members and team primary PI and instructors to promote the progress of the project.
  • Project Design
    • Propose ideas, search data, design plasmids and plan experiments with team members. Summarize and organize the primer list.
  • Wet Lab
    • Participate in the daily meeting of the Wet Lab group, discuss and solve problems with the team members.
    • Coordinate the writing of Part Registry related content, and ultimately responsible for Part Registry.
  • Wiki
    • Organize and coordinate all team members to write wiki content.
    • Participated in the editing of some web pages.
    • Responsible for part of the production of pictures and file upload.
  • Modeling
    • Discussed with team members and decided on the topic of modeling. Searching materials and participating in the work of modeling description.
    • Organize team members to discuss modeling issues with Macao local brewery technicians.
  • Human Practice
    • Participated in the interview of the director of the Macau DSEDT.
    • Participated in the Local High School talk, responsible for the introduction of our project.
    • Participated in the iGEM Düsseldorf's team’s postcard collection.
    • Involved in the picture book activity held by NNU_China.
    • Responsible for the purchase of materials needed for the Human Practice work with the Secretary group, like postcart and mask.
  • Outreach
    • Contact with the local brewery.
    • Contact with other teams for collaboration and partnership.
  • Video
    • Participated in modifying the manuscript of the promotion video, and took charge of the shooting and editing of the real scene part of the video.
    • Responsible for the production of the story part of the presentation video.

Wet Lab

Jason, ZHU DINGCHEN

  • Initial Idea Proposal
    • Proposing introducing chit42 gene to transform yeast with specific antioxidant function.
    • Concepted the original idea and coordinated teammates to shape the final project.
  • Lab Works
    • PCR amplification, Gibson assembly, DNA gel running, DNA gel purification, heat shock E. coli transformation, miniprep to extract plasmid, yeast transformation, Colony picking, yeast colony PCR, western blot, protein purification, DPPH, and pyrogallol function assay for chit 42.
  • Lab Log Writing
    • Writing and recording lab data, help to write some of the protocols, etc, function assay.
  • Online Conferences
    • Participated in some of the online conferences, such as bonus engineering webinar: lab automation.
  • Diagnostic and Problem Solving
    • Optimizing function assay systems, discerning problems, and seeking solutions with other members. coordinated with the professor and discussed the potential cause.
  • Data Processing and Analysis
    • Processed data like the function assay, Supervision of teammates for the laboratory works.
  • Inventory and Stock Management
    • Mainly in charge of keeping track of the amount of materials that we would need for each experiment and replenishing them if necessary.
  • Wiki
    • Involving in WIKI edit.

April, QI RUI

  • Design
    • Search data and proposing the initial idea of introducing the SOD1 gene to transform yeast with antioxidant function.
    • Design transformation details, plasmids construction, and insertion fragments for inducting target genes to yeast.
    • Design primers and prepare materials for sequencing plasmids we constructed.
  • Lab Works
    • PCR amplification, Gibson assembly, DNA gel running, DNA gel purification, heat shock E. coli transformation, miniprep to extract plasmid, yeast transformation, Colony picking, yeast colony PCR, measure the yeast growth curve of genomic edited yeast and wild type yeast in two different media, western blot, protein purification, DPPH, pyrogallol and other function assays for SOD1 GM yeast.
  • Data Analysis
    • Calculate data and draw the trend line of SOD1 function assay, label and collate the results of PCR and western blot.
  • Lab Affairs Management
    • Organize and host the wet every day Lab meeting.
    • Mainly arrange the experimental progress and assign other team members.
    • Writing and recording lab data, help to write some of the protocols, etc, function assay.
    • Participated in some of the online conferences, such as the bonus engineering webinar: lab automation.
    • Mainly in charge of organizing and storing experiment stocks and reagents.
  • Diagnostic and Problem Solving
    • Try to solve the problems in the process of constructing plasmids and transforming insertion fragments to yeast.
    • Optimize function assay systems by discerning problems and seeking solutions with supervisor and other members.
    • Coordinated with the supervisors, discussed the potential cause, and finally put forward some solutions.
  • Other
    • Responsible for writing design, engineering, result, and other parts in the wiki and SOD1 related content in Promotion Video.

Cecilia, YIN JIAJIE

  • Wet Lab
    • Wrote the description of promotion video.
  • Lab Works
    • Participate in constructing the plasmid for E. Coli transformation, E.Coli heat shock transformation, including PCR, prepare agarose gel, purification, miniprep, restriction digest, transformation, plasmid extraction, colony PCR, gel electrophoresis, yeast transformation.
    • Wrote the proof of concept and proposed implementation with other members.
  • Video
    • Participated in dubbing the presentation video.

YU DI

  • Wet Lab
    • Constructing the plasmid for E. Coli transformation, E. Coli heat shock transformation, plasmid extraction, colony PCR, sending the plasmid to sequencing, gel electrophoresis, yeast transformation, protein extraction for yeast cell and supernatant, western blot, pyrogallol function assay, DPPH function assay, PNP function assay.
    • Find literature and discuss with the supervisors when new obstacles occurred.
    • Data analysis after doing the function assay.
  • Media
    • Constructed the mascot image and project logos.
    • Drawing the background image for the promotion video.
  • Wiki
    • Writing the Wiki content.

Andy, CHEN LIANG YING

  • Wet Lab
    • Constructing the plasmid for E. coli transformation(PCR gel purification), E. Coli heat shock transformation(Gibson), plasmid extraction, colony PCR, yeast transformation.
  • MCollaboration and Partnership
    • Attend the meet-up meeting with other Yeast Alliance teams.

Evelyn, YU EVELYN WING LAM

  • Wet Lab
    • Participated in constructing plasmids with the interest gene, SOD1, PNP1, and CHIT42, for E.coli transformation by using PCR, gel electrophoresis, gel purification, miniprep, Gibson assembly, transformation.
    • Recorded the lab notes and data during the experiment.
    • Wrote the proof of concept and proposed implementation with other members.
  • Video
    • Wrote the description for the promotion video.
    • Participated in dubbing the presentation video.

Sunny, SU TING

  • Wet Lab
    • Participate in the experiment of introducing three genes into yeast including PCR, prepare agarose gel, purification, miniprep, restriction digest, transformation, Write the content of the proof of concept, and proposed implementation with other members of the wet lab group. Help to finish the part registry.
  • Human Practice
    • Prepare the PPT for the lecture and go to the local high school in Macau for the lecture.
    • Participated in dubbing the presentation video.

KANG HEMING

  • Wet Lab
    • Mainly responsible for transforming SOD1, CHIT42, PNP1 genes, and pFA6a-3HA-KanMX plasmid into DH5alpha cells using some techniques like .generation of competent cells, PCR, gel electrophoresis, gel purification, miniprep, Gibson assembly, transformation, and so on to prepare for gene sequencing and function assay.
  • Collaboration and Partnership
    • Attend the meet-up meeting with other Yeast Alliance teams.
    • Collaborate with CPU team members.
    • Contribute to the yeast handbook to provide help to teams that use yeast in the future.

Lorre, FU HONGKUN

  • Project Design
    • Propose ideas, search data, design plasmids and plan experiments with team members. Design and write the protocol of function assay part of PNP1 gene.
  • Lab Work
    • Constructing the plasmid for E. coli transformation, E. Coli heat shock transformation, plasmid extraction, colony PCR, gel electrophoresis, yeast transformation, protein extraction for yeast cell and supernatant, western blot, PNP function assay.
    • Record the lab note, experiment data and data analyze.
    • Problem solvin.

IT

Charles, TANG SONGXIANG

  • Team Affairs
    • Planning, assigning, Instructing, and participating in the implementation of media production and modeling.
  • Wiki
    • Together with team members, build the structure of the wiki website, mainly coding, and scriptwriting.
  • Modeling
    • Doing some data analysis. Design, calculate and construct the mathematical model with team members.
  • Video
    • Participate in animated video production and music composition.

Iris, XU SIHAN

  • Human practice
    • Wrote the script of the promotion video.
  • Wiki
    • Editing parts of the web pages.
  • Modeling
    • Visited the local brewery and discussed the modeling issues with them.
    • Searched relevant materials, participated in the work of modeling description.
  • Video
    • Made the caption of the promotion video.
    • Participated in making the caption of the presentation video.

Vivian, TAN XIAOWEI

  • Project Design
    • Propose ideas, search data, design plasmids, and plan experiments with project designers.
  • Wiki
    • Write codes to design the iGEM wiki web page.
  • Modeling
    • Related data searching, propose functional ideas, write descriptions.
  • Promotion Video
    • Draw elements, edit video, make animation.
  • Video
    • Dub for the presenting video.
  • Outreach
    • Visit the beer factory for problem collection.
  • Picture book
    • Draw a book for the UM_iGem project to tell the whole story.

Romy, LIN YUWEN

  • Wiki
    • Design parts of wiki web pages.
  • Modeling
    • Discussed modeling issues with team members, searched related information, and participated in writing descriptions.
  • Video
    • Participated in animated video production.

Human Practice

Daisy, ZHANG QIYUE

  • Project Design
    • Propose ideas, search data, and promote Human Practice with team members.
  • Human Practice
    • Participated in the question design of the interview with the directors of Macau.
    • Participate in the design and analysis of beer questionnaires.
    • Prepare a business plan.
  • Wiki
    • Compile relevant parts of human practice.
  • Media
    • Responsible for the operation of the WeChat official account.
  • Outreach
    • Visits and visits to local breweries.
  • Video
    • Responsible for the shooting of the real part of the film.

Nana, LIANG QIWEN

  • Task allocation and members coordination.
  • Responsible for contact iGEM official.
  • Collected the photos of our album from other teams.
  • Prepared for the interview with the director for economic development.
  • Prepared the lectures for students in other school in Macao.
  • The presentation video production was completed by me and other team members.

Ceylon

  • Dub for the video.

Melinda, LI MENGDI

  • Human Practice
    • Made a questionnaire to collect people’s interest in our project.
    • Held an activity to collect the improved beer’s logo.
  • Video
    • Wrote a description of the promotion video.
  • Wiki
    • Wrote related things of human practice.

Holly, HE XIAONAN

  • Questionare
    • Made up a questionnaire to have a deeper understanding of people's acceptance of gene-edited beer as well as publicizing why low purine beer is better for our bodies.
  • Dubbing
    • Dubbing for the Cantonese version promotional video to let more people know about our project. Firstly introduce the beer market situation of Macau. Secondly, explain why we have to decrease purine in beer and beer oxidation and what benefit will our project bring to beer companies. Thirdly tell the audience that our project involves the chit42, PNP1, and SOD1 genes and how we put these three genes into the yeast.
  • Collection
    • Collect the accompanying beer snacks, our cooperation teams sent to us. Then make them an accompanying beer snack collection and introduce these accompanying beer snacks briefly as well as introduce the beer culture of other countries at the same time.
  • Wiki
    • Write the description of the accompanying beer snack collection. To describe how we make the collection and what the collection is about.
  • Interview
    • Participated in the interview of the director of the Macau DSEDT. To know more about the policy and government’s view about the beer market.

Secretary

Jackie, ZHANG XUAN

  • Made the iGEM deadline calendar.
  • Outreach
    • Made the outreach budget plan with Human practice members.
    • Responsible for the purchase of related items.
    • Designed the postcards for outreach.
  • Presentation video
    • Wrote the presentation video’s story.
    • Made the PPT used in the presentation video and wrote the script with Secretary members.
    • Participated in the editing of the video.
  • Human Practice
    • Held the activity to collect the improved beer’s logo with Human practice members.
  • Wiki
    • Wrote the background part with Secretary members.

Dorothy, CAI YICHENG

  • Made the iGEM deadline calendar.
  • Wiki
    • Wrote the background part of ”relationship between beer and gout”.
    • Wrote the background part of “Current situation of the beer market in Macau”

Lorraine, YANG LUOYI

  • Video
    • Contribute to the translation of the subtitle from the Chinese version to the English version.
  • Proposal
    • Collect the materials related to services and products provided by the beer company and contribute in part of the writing.
  • Wiki
    • Help in writing, searching, and grammar checking.

NJ, MA CHINWA

  • General
    • Mainly responsible for internal affairs and coordinating with other iGEM teams.
  • Human practice
    • Coordinate and implement the human practice work, and prepare relevant publicity materials.
  • Public Engagement
    • Design, develop and implement related activities. Activities include an event co-organized with iGEM teams from different countries such as Germany, Greece, and the United States: a photo album collecting the pictures of different dishes that go with the beer in different areas. Made the design of the cover and part of the inner page.
  • Presentation Video
    • Prepare for presentation video PPT and scripts.
  • Wiki
    • Write relevant content in the wiki.

Principal Investigators

Acknowledgement of Faculty of Health Science (FHS)

    Thanks to Faculty of Health Science (FHS) for providing lab space, consumable, equipment and sponsor for this project. FHS also provided us with the basic experiment equipment, reaction agents, and kits for different experiments like western blot system, DNA gel purification kit, EP tube and pipette tips, etc.

Prof. Leo, TSZ ON LEE

    As the convener of our team, Prof Leo was responsible for administrative procedures and provided most of the suggestions on the experiments and human practices and assist in project design.

Prof. Chris, KOON HO WONG

    Prof WONG is an expert in fungi and provided us a lot of valuable advice on project design. He also helped us along the whole experiment process.

Prof. KIN YIP TAM

    Prof TAM gave many suggestions on improving our function assay and modeling.

FHS staff

Stephanie, PEI WEN NG

    She assisted our team in all the administrative procedures including iGEM team registration, financial application, and others, to ensure the successful establishment and maintenance of our team.

Special Acknowledgement

Philip Naderev P. LAGNITON

    The iGEMer from UM_Macau 2019. He gave a lot of advice on design and checking files.

JING TONG TAN

    The iGEMer from UM_Macau 2019. She gave advice on figure design and helped us with files.

Aggie, FENG YU ZHAO

    The iGEMer from UM_Macau 2020. She gave a lot of advice in our whole project process.

IAN SAM LOK

    He is the lab instructor in the University of Macau. He guided and helped us a lot in the process of experiments.

LAN QING, DONG ZHI QIANG

WANG MEI LIN, WEI XIAN YUAN

    They are PhD students of the University of Macau. They have guided and helped us a lot in the process of experiments.

CONTACT US

igemum_macau@outlook.com

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