Team:ULaval/Experiments

Project

Project Description Experiments Parts Results Safety Project Modelling Engineering Success Proof of Concept Syrup Microbiology

Human Practices

Human Practices Implementation

Team

Team Attributions Notebook

Collaboration & Communication

Education & Communication Collaboration Contribution

Collaboration & Communication

Education & Communication Collaboration Contribution

Project

Project Description

The Basics

Experiments Parts Results Safety

What Makes It Great

Project Modelling Engineering Success Proof of Concept Syrup Microbiology

Human Practices

Human Practices Implementation

Team

Team Attributions Notebook

Collaboration & Communication

Education & Communication Collaboration Contribution

Experiments

Medium and reagents

2YT + glucose

LB

Solutions for agarose gels

Preparation Nelson-Somogyi reagent

Cloning

Competent bacteria cells preparation

Competent bacteria cells transformation

Bioshop Taq PCR

Glycerol stock for E. coli

Protein expression and purification

Optimization of protein expression

Verification of protein solubility

Protein expression

Protein purification

Bradford assay

Enzymatic assays

Standard curve of glucose equivalent

Determination of enzymatic unit

Determination of optimal pH

Determination of optimal temperature

Determination of pH stability

Determination of temperature stability

Rheological assays

Rheological assays

Microbiology characterization

Microorganisms isolation

16S & 18S PCR amplification