Team:Tianjin/Parts

Parts Overview

We designed 32 parts. This amount of parts is not only great for our project, but more importantly this will be a huge advantage for all further iGEM-Teams. By sharing our part collection with the iGEM community, other teams can easily incorporate parts in their projects. The standardized overhangs of the constructs allow an exchange between every lab.

Composite part of the CREATE

In order to degrade Saccharomyces cerevisiae’s chromosomes to form CREATE, we constructed several different composite parts to regulate Cas9 protein expression. Their regulatory mechanisms and formation efficiency are different.

See their results

Name	Type	Description	Length(bp)
BBa_K3939666	Composite	Gal Cas9	4597
BBa_K3939998	Composite	Cas9 with flipped promoter	4638

GFP part for characterization

In order to characterize the formation of CREATEs, we designed a fast-degrading GFP and insert the gene into S.cerevisiae chromosomes. In this situation, no new GFP was produced after the chromosomes were cleaved.

See their results

Name	Type	Description	Length(bp)
BBa_K3939111	Composite	Fast degrading GFP	1035

Parts Table

Basic Parts

Name	Type	Description	Length(bp)
BBa_K3939000	DNA	Delta sites	20
BBa_K3939001	Coding	Cas9 with NLS label	4140
BBa_K3939002	DNA	GFP’s left homologous arm	300
BBa_K3939004	Coding	Ubiquitin	318
BBa_K3939005	Coding	Ura	804
BBa_K3939006	Terminator	GFP Terminator	236
BBa_K3939007	Regulatory	pGal promoter	457
BBa_K3939008	Coding	tetR gene expression product	624
BBa_K3939009	DNA	LoxP sequence	34
BBa_K3939010	DNA	GFP right homologous arm	300
BBa_K3939011	DNA	tetR gene expression product binding site	19
BBa_K3939012	Regulatory	Ura Promoter	221
BBa_K3939013	Terminator	Ura Terminator	130
BBa_K3939015	Terminator	CYC1 terminator	229
BBa_K3939016	Regulatory	flipped pGal promoter	430
BBa_K3939017	Regulatory	TEF1 promoter	348
BBa_K3939020	Regulatory	Gai-tetO promoter	449
BBa_K3939021	Composite	Fast degrading GFP with homologous arms	3563
BBa_K3939030	Regulatory	ADH1 Promoter	705
BBa_K3939031	Terminator	ADH1 Terminator	188
BBa_K3939032	DNA	GFP’s right	415

Composite Parts

Name	Type	Description	Length(bp)
BBa_K3939111	Composite	Fast degrading GFP	1035
BBa_K3939222	Composite	GFP with homologous arms	3792
BBa_K3939233	Plasmid	Carotene regulated by the tetR-tetO regulatory system	16014
BBa_K3939235	Plasmid	Carotene-loxp	15968
BBa_K3939666	Composite	Gal Cas9	4597
BBa_K3939887	Composite	Cas9 gene regulated by tetR	5790
BBa_K3939998	Composite	Cas9 with flipped promoter	4638
BBa_K3939021	Composite	Fast degrading GFP with homologous arms	3563