Team:Tianjin/Analysis
Details of HP
Producing drugs
△Chemical method
△Biological fermentation
△Cell-free system
2.We learned the strength and weakness of current methods.
△Strength: Cheap and longer life expectancy.
△Weakness: A large part of carbon and nitrogen sources are used to maintain life activities rather than production.
3. We communicated the advantages about CREATE in producing drugs.
△Many industrial bacteria are very rigid and difficult to be modified, and modified genes might be silenced. Yeasts are relatively easy to be transformed.
△There is no unnecessary carbon and nitrogen source diversion.
△There will be no disclosure information in CREATE.
△Increase the life span of CREATE by adding metabolic pathway.
△New design methods are needed to avoid possible metabolic disorders and decreased activity.
△Need to know how to get plenty of CREATEs.
△Verify whether the function of membrane protein can be maintained.
Drugs delivery
1.We informed the current methods of drug delivery.
At present, one of the best technologies is nano drug technology. At present, about 40% of drugs are hydrophobic drugs. Because the human body is a water environment, we must change this drug from hydrophobic to hydrophilic. We can solve the problem of hydrophobicity by loading drugs into a ball or a shell, that is, into the carrier to make nano drugs.
2.We learned what needs to be done if delivering drugs into human body.
△Oral administration: We need to consider the anti-gastric acid ability of drugs and the digestion ability of many enzymes in the intestine.
△Intradermal injection: First of all, it is necessary to ensure whether the drug has efficacy after entering the body. Secondly, we should ensure safety and not be toxic to normal cells. Third, we need to consider whether the drug can maintain the stability of the carrier in the plasma.
3.We are communicated the advantages and disadvantages about CREATE compared with existing technology.
△Advantages: Naturally targeted.
△Disadvantages: Proteins on the surface are easy to inactivate.
4.We discussed what needs to be improved later.
△Genetic material inactivation.
△Retain active after drug delivery. For example, it cannot be degraded by enzymes in the gastrointestinal tract or attacked by immune cells in the human body.
△It can be made into a kit to accurately quantify sustained-release drugs.
△Alleviate immune response.
Transform large DNA fragments
1. We informed the current methods of large fragment DNA delivery.
△Chemical transfer
△electric transfer
△protoplast transfer
2. We communicated the advantages about CREATE in large fragment DNA delivery.
△Large DNA fragments can be transferred at one time.
△After the fusion of two haploid yeast, a set of chromosomes will be lost randomly. Although the yeast can be forced to selectively lose the one we don't want by adding screening conditions, there may not always be appropriate screening conditions. At this time, it will be a good choice to remove the yeast chromosomes by CRISPR Technology.
3.We discussed what needs to be improved later.
△When delivering large fragments of DNA, cells can be fused before cutting, so as to improve the efficiency of homologous recombination.
△Make a regulatory switch to prevent the transferred DNA from affecting the physiological state of chromosome free cells.
△How to screen whether the bacteria is transformed into large DNA fragments or not.