Team:TU Kaiserslautern/Experiments
MOCLO MANIA
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EXPERIMENTS
Basic Protocols
Leishmania
Read
BHI agar medium
BHI freezing medium
BHI medium original cells
BHI medium after transfection
Buffers
Culture preparation for purification
Dilution of cell cultures
Freezing cell cultures
Immunoblot
Tetracycline Induction
mCherry spectrum
Picking clones
Plating out cell cultures
Stripping membranes
Transfection
Transfection Buffer
MoClo
Read
1% HD Green Agarose gel
2% HD Green Agarose gel
50x TAE
Competent Making
Diagnostic Digest
DMSO Stock
DNA precipitation
Gel Extraction
HD Green Poststain
Inoculation
LB agar plates
Modular Cloning
Plasmid Miniprep
PCR using Q5 DNA Polymerase
Ligation using T4 DNA Ligase
TOP10 E. coli Transformation
Protein
Read
Antibody staining
Ammonium sulfate precipitation
GST Purification
His Purification
Chemiluminescence Detection using HRP
SDS gels
Strep Purification
TCA precipitation
Western Blot 1
Western Blot 2
Preparing human cells for Western Blot
Labjournal
Leishmania
Read
MoClo
Read
Protein
Read
Activity Assay
Read
