Team:Linkoping Sweden/Handbook
Phototroph Handbook
Due to a lack of standardization when working with phototrophs, we, together with many of the iGEM 2021 teams working with phototrophic organisms, created a phototrophic handbook to help future iGEM teams. This 161 pages long handbook, spearheaded by iGEM Marburg and iGEM Bielefeld-CeBiTec, contains a lot of useful information that will help future teams when working with phototrophs such as algae, cyanobacteria, and plants. From the handbook, future iGEM teams will get information regarding acquiring organisms, cultivation, transformation, and cloning of the different chassis they choose.
We in iGEM Linköping wrote the chapter Growth and Cultivation of cyanobacteria, where we included experience and data acquired regarding working with Synechocystis sp. PCC 6803, throughout our project. Topics such as proper incubator setup, growth media, and general environment are discussed in this chapter. The goal is for future iGEM teams that plan on working with Synechocystis sp. PCC 6803 to be able to use our gathered information to more easily get started with their wet lab work.
During the course of this project, we have had four big meetups with several other phototroph teams, a community we have chosen to call iGEM – Phototrophs. During these meetings, the handbook has been discussed. In addition to this, experts have been invited to give lectures about interesting and helpful topics regarding phototrophs. Additionally, we received feedback on our projects. At one of the meetups, our team held a presentation about some of the challenges we had encountered. Based on this, the other teams were able to provide us with useful information and tips regarding our transformation method. The badges seen below were awarded to the teams that were a part of the iGEM Phototrophs community. The community badge was awarded to all iGEM Phototrophs teams, the Handbook badge to the teams that contributed to the Phototroph Handbook, and the Presentation badge was awarded to the teams that presented during the meet-ups.
