Due to a lack of standardization when working with phototrophs, we, together with many of the iGEM 2021 teams working with phototrophic organisms, created a phototrophic handbook to help future iGEM teams. This 161 pages long handbook, spearheaded by iGEM Marburg and iGEM Bielefeld-CeBiTec, contains a lot of useful information that will help future teams when working with phototrophs such as algae, cyanobacteria, and plants. From the handbook, future iGEM teams will get information regarding acquiring organisms, cultivation, transformation, and cloning of the different chassis they choose.
We in iGEM Linköping wrote the chapter Growth and Cultivation of cyanobacteria, where we included experience and data acquired regarding working with Synechocystis sp. PCC 6803, throughout our project. Topics such as proper incubator setup, growth media, and general environment are discussed in this chapter. The goal is for future iGEM teams that plan on working with Synechocystis sp. PCC 6803 to be able to use our gathered information to more easily get started with their wet lab work.