Team:Hong Kong JSS/Composite Part
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Team:Hong Kong JSS/Composite Part
Hong Kong JSS
Composite parts
In our project, we designed composite parts for the expression and secretory expression of our GOI - tvLac and FDR-A.
In the expression constructs, the coding sequence of our GOI is driven by T7 promoter (BBa_I719005), a lac operon (BBa_K3076802), strong RBS (BBa_K3076801), and a T7 terminator (BBa_K395601).
The GOI proteins are tagged with a 6-His Tag (CATCATCATCATCATCAT) for protein purification.
In the secretory constructs, either signal peptide DsbAss (BBa_K3746005) or NSP4 (BBa_K3746004) were added to the N-terminal for the protein secretion.
Please refer to the Part Registry for detailed information about the parts.( Part List)
In the expression constructs, the coding sequence of our GOI is driven by T7 promoter (BBa_I719005), a lac operon (BBa_K3076802), strong RBS (BBa_K3076801), and a T7 terminator (BBa_K395601).
The GOI proteins are tagged with a 6-His Tag (CATCATCATCATCATCAT) for protein purification.
In the secretory constructs, either signal peptide DsbAss (BBa_K3746005) or NSP4 (BBa_K3746004) were added to the N-terminal for the protein secretion.
Please refer to the Part Registry for detailed information about the parts.( Part List)