ALIGNMENT: B.subtilis D Primer52-alignment
ALIGNMENT: B.subtilis D Primer43-alignment
ALIGNMENT: B.subtilis C Primer44-alignment
ALIGNMENT: B.subtilis C Primer47-alignment
ALIGNMENT: B.subtilis B Primer51-alignment
ALIGNMENT: B.subtilis B Primer46-alignment
PROTOCOL:_Subcloning and transformation of Escherichia coli host
PROTOCOL: AmyE test (Starch hydrolysis test)
PROTOCOL: Isolation of plasmids
PROTOCOL: Long-Range PCR
PROTOCOL: Preparation of competent cells of Bacillus subtilis
PROTOCOL: Preparation of competent cells of BL21(DE3)
PROTOCOL: Preparation of glycerol stocks
PROTOCOL: Preparation of growth media
PROTOCOL: Proof of the successful transformation of Bacillus subtilis
PROTOCOL: Proof of transformation of E.coli
PROTOCOL: Test of competent cells
PROTOCOL: Transformation of Bacillus subtilis
TABLE: Table of samples for measurement
TABLE: Tecan-settings and optimization
Igem Team Brno, Czech Republic 2021
