Team:British Columbia/Parts
EXPERIMENTS
Parts
| PART NAME | TYPE | DESCRIPTION | LENGTH (bp) |
|---|---|---|---|
| psicA | Basic (promoter) | sicA promoter that serves as a regulatory element in the Salmonella SPI-1 secretion system | 143 |
| Golden Gate compatible psicA | Basic (promoter) | sicA promoter with BsaI cut sites | 181 |
| pSB1C3-BBa_J04450 | Composite | RFP generator driven by plac promoter | 3076 |
| pLactate | Basic (promoter) | promoter that is part of the lactate regulatory unit in Salmonella; regulated by Lldr | 218 |
| lldR | Regulatory | transcriptional regulator of lactate regulatory unit; represses pLactate (promoter) in the absence of lactate | 777 |
| pLactate-RFP-lldR | Composite | reporter construct made to characterize the activity of the lactate inducible regulatory unit; it consists of the pLactate, RBS, a synthetic promoter, and lldR, RFP, and two terminators. LldR is constitutively expressed, through the synthetic promoter | 2071 |
| pSicA-luxABG | Composite | psicA promoter upstream of lux operon genes luxA, luxB, and luxG. psicA is a promoter that is part of the lactate operon in Salmonella; regulated by Lldr | 2045 |
| pLactate-luxCDE-lldR | Composite | pLactate promoter upstream of lux operon genes luxC, luxD, and luxE. psicA is a promoter that is part of the lactate operon in Salmonella; regulated by Lldr | 4837 |
| pBAD-luxABG | Composite | pBAD promoter upstream of lux operon genes luxA, luxB, and luxG. The pBAD promoter is derived from the L-arabinose expressing operon of E. coli | 4068 |
| pBAD-luxCDE-lldR | Composite | the pBAD promoter is derived from the L-arabinose expressing operon of E. coli. | 5829 |