Contribution
BBa_K3996004
== Profile ==
Name: AnXlnB orf
Base Pairs: 678bp
Origin: synthesis
Properties: Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
==== Usage and Biology ====
This protein is involved in the pathway xylan degradation, which is part of Glycan degradation. Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.
== Construct design ==
The plasmid is engineered for further use. (Figure 1).
Figure 1. DNA map of plasmid pXylan-B.
BBa_K3996007
== Profile ==
Name: pOdd-1
Base Pairs: 1770bp
Origin: synthesis
Properties: A Yeast Expression plasmids backbone
== Usage and Biology ==
Wheat B starch is a by-product of wheat starch deep processing, which is often directly used as feed, with low industrial added value. If wheat B starch is used as raw material to produce alcohol, part of the shortcomings of wheat starch alcohol can be avoided and the utilization value of wheat B starch can be improved.
After sugar production of wheat B starch by liquid saccharification pretreatment, it can use conventional brewing yeast to produce alcohol, but this process composition of pentosan in wheat B starch did not use, even in the pretreatment stage to join pentosan enzyme, xylose and arabinose (pentose monosaccharides will use by conventional saccharomyces cerevisiae, at the same time the extra pentosan enzyme also increases the cost of production. Therefore, it is ideal to develop saccharomyces cerevisiae strains with the ability of autocrine pentosanase and pentose utilization.
Figure1. Principle diagram of pentosan fermentation.
== Construct design ==
We reconstructed the plasmid pAR318 by PCR for further use.
Figure 2. DNA sequence map of plasmid pOdd-1.
BBa_K3996012
== Profile ==
Name: pXynA
Base Pairs: 2631bp
Origin: synthesis
Properties: A Yeast Expression plasmids backbone
==== Usage and Biology ====
It is formed with pOdd-3 plasmid backbone, FBA1 promoter, AnXlnD orf and CYC1 terminator.
== Construct design ==
The plasmid is engineered for further use. (Figure 1).
Figure 1. DNA map of plasmid pXylan.
BBa_K3996013
== Profile ==
Name: pXylan-B
Base Pairs: 1593bp
Origin: Saccharomyces cerevisiae, synthesis
Properties: pentosan fermentation to produce alcohol
== Usage and Biology ==
Wheat B starch is a by-product of wheat starch deep processing, which is often directly used as feed, with low industrial added value. If wheat B starch is used as raw material to produce alcohol, part of the shortcomings of wheat starch alcohol can be avoided and the utilization value of wheat B starch can be improved.
After sugar production of wheat B starch by liquid saccharification pretreatment, it can use conventional brewing yeast to produce alcohol, but this process composition of pentosan in wheat B starch did not use, even in the pretreatment stage to join pentosan enzyme, xylose and arabinose (pentose monosaccharides will use by conventional saccharomyces cerevisiae, at the same time the extra pentosan enzyme also increases the cost of production. Therefore, it is ideal to develop saccharomyces cerevisiae strains with the ability of autocrine pentosanase and pentose utilization.
== Construct design ==
The plasmid is engineered for further use. (Figure 2).
BBa_K3996014
== Profile ==
Name: pXylan-BD
Base Pairs: 4842 bp
Origin: Saccharomyces cerevisiae, synthesis
Properties: pentosan fermentation to produce alcohol
== Usage and Biology ==
Wheat B starch is a by-product of wheat starch deep processing, which is often directly used as feed, with low industrial added value. If wheat B starch is used as raw material to produce alcohol, part of the shortcomings of wheat starch alcohol can be avoided and the utilization value of wheat B starch can be improved.
After sugar production of wheat B starch by liquid saccharification pretreatment, it can use conventional brewing yeast to produce alcohol, but this process composition of pentosan in wheat B starch did not use, even in the pretreatment stage to join pentosan enzyme, xylose and arabinose (pentose monosaccharides will use by conventional saccharomyces cerevisiae, at the same time the extra pentosan enzyme also increases the cost of production. Therefore, it is ideal to develop saccharomyces cerevisiae strains with the ability of autocrine pentosanase and pentose utilization.
== Construct design ==
We selected the codon-optimized xylanase gene AnXlnB, the β-xylanase gene AnXlnD, and the acetylxylanase gene CcXynA. Saccharomyces cerevisiae strains with the decomposition and utilization capacity of pentosan were obtained. The ability of pentosan fermentation to produce alcohol was tested in a specific medium.(Figure 2).
Figure1. Principle diagram of pentosan fermentation.
BBa_K3996015
== Profile ==
Name: pXylan
Base Pairs: 7473bp
Origin: Saccharomyces cerevisiae, synthesis
Properties: pentosan fermentation to produce alcohol
== Usage and Biology ==
Wheat B starch is a by-product of wheat starch deep processing, which is often directly used as feed, with low industrial added value. If wheat B starch is used as raw material to produce alcohol, part of the shortcomings of wheat starch alcohol can be avoided and the utilization value of wheat B starch can be improved.
After sugar production of wheat B starch by liquid saccharification pretreatment, it can use conventional brewing yeast to produce alcohol, but this process composition of pentosan in wheat B starch did not use, even in the pretreatment stage to join pentosan enzyme, xylose and arabinose (pentose monosaccharides will use by conventional saccharomyces cerevisiae, at the same time the extra pentosan enzyme also increases the cost of production. Therefore, it is ideal to develop saccharomyces cerevisiae strains with the ability of autocrine pentosanase and pentose utilization.
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