Team:Aix-Marseille/part-collection

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Part collection


Our part collection is composed of 12 basic parts and 12 composite parts. Every parts are distributed by use category (1. Concanavalin A : way to virus detection; 2. Concanavalin A : Exportation system to the extracellular membrane, 3. Timer lysis device and 4. Bacillus thuringiensis toxins and mosquitoes death).

- All parts were generated during our project.

Best basic part

lexA-repressed promoter : BBa_K3788017

We have chosen to present lexA-repressed promoter as our Best Basic Part. It is a promoter induced in response to DNA damage and allows gene expression in a dose-dependant manner. This promoter allows Colicin A’s gene expression, an E. coli toxin. In particular, it is constituted from -35 and -10 boxes, with SOS response consensus sequences. Plus, we can find the start of transcription coming from the caa-cal operon, used in the biological ColicinA’s timer.

Basics parts

Tables shown below contain all the basics parts. They are distributed by use category (1, 2, 3 et 4). You may find the part name that links to the part page in the registry, part type, short description, and length.

Concanavalin A : way to viruses detection
Name part Type Description Length
BBa_K3788007 Coding ConA protein equipped with a 6x HIS affinity tag 888 bp
BBa_K3788005 Coding ConA-linker protein equipped with a 6x HIS affinity tag 997 bp
BBa_K3788006 Coding ConA protein without ATG 870 bp
Concanavalin A : Exportation system to the extracellular membrane
Name part Type Description Length
BBa_K3788010 Coding Aida-I protein 1326 bp
BBa_K3788011 Coding Aida-I (D33:N) protein 1326 bp
Timer lysis device
Name part Type Description Length
BBa_K3788017
Regulatory LexA-repressed promoter 223 bp
BBa_K3788018 Coding Timer-lysis device 1487 bp
BBa_K3788015 Coding Modified lysis device 1351 bp
BBa_K3788016 Coding Timer-lysis device version 2 2225 bp
Bacillus thuringiensis toxins and mosquitoes death
Name part Type Description Length
BBa_K3788000 Coding Cyt1Aa protein equipped with a Strep affinity tag II 777 bp
BBa_K3788001 Coding Cry11Aa protein equipped with a Flag affinity tag 1956 bp
BBa_K3788002 Coding P20 protein equipped with a x6 affinity tag 570 bp

Composite part

Tables shown below contain all the composite parts. They are distributed by use category (1, 2, 3 et 4). You may find the part name that links to the part page in the registry, part type, short description, and length.

Concanavalin A : way to viruses detection
Name part Type Description Length
BBa_K3788024 Coding ConA_linker_ConAprotein equipped with a x6 affinity tag 1867 bp
Concanavalin A : Exportation system to the extracellular membrane
Name part Type Description Length
BBa_K3788025 Coding PelB_sfGFP protein 786 bp
BBa_K3788026 Coding PelB_sfGFP_ Aida-I protein 2106 bp
BBa_K3788027 Coding PelB_sfGFP_ Aida-I (D33:N) protein 2106 bp
BBa_K3788013 Coding IPTG inducible promoter with RBS, PelB_sfGFP 1012 bp
BBa_K3788014 Coding IPTG inducible promoter with RBS and GFP 946 bp
Timer lysis device
Name part Type Description Length
BBa_K3788019 Coding expression of GFP and of the timer device under the control of the LexA-controlled promoter BBa_K3788017, E0040, BBa_K3788018 2430 bp
BBa_K3788020 Coding expression of the timer device fused to the RFP protein, under the control of the LexA-controlled promoter BBa_K3788017, BBa_K3788015, E1010 2280 bp
BBa_K3788021 Coding expression of GFP and of the timer device fused to the RFP protein, under the control of the LexA-controlled promoter BBa_K3788017, E0040, BBa_K3788015, E1010 3000 bp
BBa_K3788023 Coding expression of GFP and of an optimized design of the timer device, under the control of the LexA-controlled promoter 3168 bp
Bacillus thuringiensis toxins and mosquitoes death
Name part Type Description Length
BBa_K3788003 Coding P20 equipped with a x6 affinity tag and Cry11Aa protein equipped with a flag affinity tag 2540 bp
BBa_K3788004 Coding P20 equipped with a x6 affinity tag; Cry11Aa protein equipped with a flag affinity tag and Cyt1Aa protein equipped with a Strep affinity tag II 3340 bp