Plasmid Construction:
PCR(polymerase chain reaction)------ Susie & Crystal & Fukuga
Restriction enzyme cutting------ Mark & Miya & Lucy
Recombinant plasmid construction------- Mark & Susie & Crystal
Transformation of the recombinant plasmid into the bacteria----- Lucy & Fukuga &
Miya
BL21 Competent Cell:
Standardization of ampicillin solution------- Crystal & Fukuga & Miya
Preparation of culture mediums-------- Lucy & Fukuga & Mark
Culture medium bacteria growth-------- Mark & Susie & Lucy
Bacteria shaking process-------- Mark & Crystal & Susie
Alkaline lysis-------- Lucy & Miya & Mark
Function Test:
DNA electrophoresis--------- Miya & Susie & Mark
DNA electrophoresis gel preparation-------- Lucy & Miya & Fukuga
Protein electrophoresis--------- Crystal & Susie & Miya
Testing of OD540 value(nanodrop)---------- Lucy & Crystal & Mark
Wiki working--------every member of the team
WeChat Official Account articles writing & page design-------- Fukuga & Susie &
Miya
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