|
|
Line 21: |
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| <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Team">Team Members</a> | | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Team">Team Members</a> |
| </li> | | </li> |
− | <li class="child-item"> | + | <li class="child-item active"> |
| <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Attributions">Attributions</a> | | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Attributions">Attributions</a> |
| </li> | | </li> |
− | <li class="child-item active"> | + | <li class="child-item"> |
| <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Collaborations">Collaboration</a> | | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Collaborations">Collaboration</a> |
| </li> | | </li> |
Line 99: |
Line 99: |
| </li> | | </li> |
| <li class="child-item"> | | <li class="child-item"> |
− | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/ProofOfConcept">Proof of Concept</a> | + | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Proof_Of_Concept">Proof of Concept</a> |
| </li> | | </li> |
| <li class="child-item"> | | <li class="child-item"> |
Line 121: |
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| <div class="page-header"> | | <div class="page-header"> |
| <img src="https://static.igem.org/mediawiki/2021/d/d2/T--Shanghai_Metropolis--bg-1.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2021/d/d2/T--Shanghai_Metropolis--bg-1.png" alt=""> |
− | <h1>Collaboration</h1> | + | <h1>Attributions</h1> |
| </div> | | </div> |
| | | |
Line 128: |
Line 128: |
| <section class="article p-t-54 p-b-54"> | | <section class="article p-t-54 p-b-54"> |
| <section> | | <section> |
− | <h1 class="title">1- Aseeker</h1> | + | <h1 class="title">Wet Lab</h1> |
− | <img class="left" src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_Metropolis--img_collaborations_1.jpg" alt="" style="width: 360px">
| + | |
− | <p>Our team have Collaborated with another iGEM team named Aseeker. Our collaboration is mainly through
| + | |
− | mutual understanding of each other's content and mutual advice to promote the process of both project.
| + | |
− | The central theme of the team's research is arsenic. Their research focuses on making dipsticks to
| + | |
− | detect arsenic and hopes to make the final product commercially available. They told us arsenic
| + | |
− | compounds, which are harmful to humans, have long been a problem for biological science. Testing for
| + | |
− | arsenic has become a central goal. In addition, they also explained their team's name: As is the
| + | |
− | chemical symbol for arsenic, while the name Ark stands for ark and hope. They are working on using
| + | |
− | genetic technology to create sensors based on e. coli to detect arsenic.</p>
| + | |
| | | |
− | <div class="half m-t-18 m-b-18"> | + | <section> |
− | <div class="left">
| + | <h2 class="title2 blue">Experiment</h2> |
− | <img src="https://static.igem.org/mediawiki/2021/e/e7/T--Shanghai_Metropolis--img_collaborations_2.jpg" alt="" | + | <table class="m-t-18 w-384"> |
− | style="height: 284px; width: auto">
| + | <tbody> |
− | </div>
| + | <tr> |
− | <div class="right" style="text-align: right">
| + | <td>PCR</td> |
− | <img src="https://static.igem.org/mediawiki/2021/a/a3/T--Shanghai_Metropolis--img_collaborations_3.jpg" alt="" | + | <td>Amy Qu, Vannie Zhang</td> |
− | style="height: 284px; width: auto">
| + | </tr> |
− | </div> | + | <tr> |
− | </div> | + | <td>OE PCR</td> |
| + | <td>Momo Wang, Jason Bi</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Agarose Gel Electrophoresis</td> |
| + | <td>Momo Wang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>DNA Gel Extraction</td> |
| + | <td>Jason Bi, Amy Qu</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Restriction enzyme double digestion</td> |
| + | <td>Yvonne Song, Amy Qu, Jim Jin, Vannie Zhang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Clonexpress Ligation reaction</td> |
| + | <td>Amy Qu, Waylon Yang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Plasmid transformation</td> |
| + | <td>Yang Zhou, Paul Niu</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Plasmid extraction</td> |
| + | <td>Yvonne Song, Momo Wang, Jason Bi</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Protein detection</td> |
| + | <td>Yvonne Song, Amy Qu, Vannie Zhang, Momo Wang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Medium preparation</td> |
| + | <td>All of us</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Wiki text</td> |
| + | <td>Yvonne Song, Momo Wang, Amy Qu</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| | | |
− | <p>During the whole discussion, EV71terminator and Aseeker illustrated the challenges and effective methods | + | <section> |
− | we have met in the research and human practice. After being informed of the fact that the secondary | + | <h2 class="title2 blue">Safety</h2> |
− | product implies a potential business value, that is, the by-product of the bacteria probably is cable of | + | <table class="m-t-18 w-384"> |
− | eliminating the residual arsenic, whose accumulation is harmful for human body. We consulted whether | + | <tbody> |
− | Aseeker would devote more time and financial resources into the R&D of the product, so as to gain some | + | <tr> |
− | informative suggestions that might be helpful in future determination. It turned out that Aseeker is | + | <td>Laboratory safety</td> |
− | intended to focus more on their main product. Moreover, EV71terminator also offered us experiences | + | <td>Jason Bi, Paul Niu, Momo Wang</td> |
− | related to human practice, such as field research, interview of the authority and charity bazaar. From | + | </tr> |
− | the discussion, we were exposed to several new points of view, and able to increase the efficiency in
| + | <tr> |
− | future practice. </p> | + | <td>Material safety</td> |
| + | <td>Amy Qu</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Application safety</td> |
| + | <td>Yvonne Song, Vannie Zhang, Jim Jin, Waylon Yang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Wiki text</td> |
| + | <td>All of us</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Notebook</h2> |
| + | <table class="m-t-18 w-384"> |
| + | <tbody> |
| + | <tr> |
| + | <td>Daily record</td> |
| + | <td>Yvonne Song, Amy Qu</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Wiki text</td> |
| + | <td>Yvonne Song, Amy Qu</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Project description</h2> |
| + | <table class="m-t-18 w-384"> |
| + | <tbody> |
| + | <tr> |
| + | <td>Inspiration</td> |
| + | <td>Yvonne Song, Amy Qu, Jim Jin</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Background</td> |
| + | <td>Momo Wang, Yvonne Song</td> |
| + | </tr> |
| + | <tr> |
| + | <td>General concept</td> |
| + | <td>Momo Wang, Yvonne Song</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Presentation</h2> |
| + | <table class="m-t-18 w-384"> |
| + | <tbody> |
| + | <tr> |
| + | <td>Background</td> |
| + | <td>Amy Qu</td> |
| + | </tr> |
| + | <tr> |
| + | <td>General concept</td> |
| + | <td>Jason Bi</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Detailed process</td> |
| + | <td>Momo Wang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Experiment results</td> |
| + | <td>Yvonne Song</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| </section> | | </section> |
| | | |
| <section> | | <section> |
− | <h1 class="title">2- Neuron Target</h1> | + | <h1 class="title">Dry Lab</h1> |
− | <img class="left m-b-18" src="https://static.igem.org/mediawiki/2021/0/06/T--Shanghai_Metropolis--img_collaborations_4.jpg" alt="" style="width: 360px"> | + | <section> |
− | <p>The second Chinese team that we collaborated with was from Nanjing, and their name is Neuron Target. The
| + | <h2 class="title2 blue">Human practice</h2> |
− | subject is also of medical interest, and the product is a compound that can be found to address the
| + | <table class="m-t-18 w-384"> |
− | inflammatory response caused by activated microglia.</p> | + | <tbody> |
− | <img class="left m-t-18 m-b-18" src="https://static.igem.org/mediawiki/2021/6/6b/T--Shanghai_Metropolis--img_collaborations_5.png" alt=""> | + | <tr> |
− | <p>We were fortunate to participate in the presentation of Neuron Target's team. They showed us what they
| + | <td>Research</td> |
− | were working on and what they were aiming for. At the same time, the team also showed us the daily life
| + | <td>Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Jacky Cai, Rachel Zhou</td> |
− | of the team lab. We got to know each other, and discussed many topics such as product development and
| + | </tr> |
− | promotion, cooperation between dry and wet teams, etc. Since our research direction is related to
| + | <tr> |
− | medicine, the audience will be similar. By taking this opportunity, we learned a lot of knowledge about
| + | <td>Public survey</td> |
− | business promotion from each other. In the end, we paid attention to each other's propaganda platform,
| + | <td>Kevin Zou, Jacky Cai, Jason Xie,Sun Han, Vivian Huang, Betty Zhang, Rachel Zhou</td> |
− | and hoped to carry out further cooperation in the future.</p> | + | </tr> |
| + | <tr> |
| + | <td>Street interview</td> |
| + | <td>Jason Xie, Kevin Zou, Carl Yuan, Rachel Zhou, Lee, Vivian Huang, Betty Zhang, Jacky Cai</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Expert interview</td> |
| + | <td>Jacky Cai, Rachel Zhou, Betty Zhang, Carl Yuan, Jacky Cai</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Business plan</td> |
| + | <td>Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Rachel Zhou, Lee,Betty Zhang, Jacky Cai</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Collaboration</td> |
| + | <td>All of us</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Wiki text</td> |
| + | <td>Lee, Rachel Zhou,Betty Zhang, Jason Xie, Carl Yuan, Jacky Cai, Rachel Zhou, Sun Han, Vivian |
| + | Huang |
| + | </td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Design</h2> |
| + | <table class="m-t-18 w-384"> |
| + | <tbody> |
| + | <tr> |
| + | <td>Logo & Team name</td> |
| + | <td>Jason Xie,Betty Zhang, Rachel Zhou</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Peripheral product</td> |
| + | <td>Jason Xie, Carl Yuan, Jacky Cai</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Publicity material</td> |
| + | <td>Rachel Zhou, Betty Zhang, Vivian Huang</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Promotion video</h2> |
| + | <table class="m-t-18 w-384"> |
| + | <tbody> |
| + | <tr> |
| + | <td>Actors</td> |
| + | <td>Carl Yuan,Sun Han,Heidi Sheng, Kevin Zou, Jacky Cai</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Script</td> |
| + | <td>Heidi Sheng, Crystal Li</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Shoot & Clip</td> |
| + | <td>Vivian Huang</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Publicity</h2> |
| + | <table class="m-t-18 w-384"> |
| + | <tbody> |
| + | <tr> |
| + | <td>Official account</td> |
| + | <td>Jason Xie, Betty Zhang</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Charity</td> |
| + | <td>Vivian Huang, Lee, Rachel Zhou,Heidi Sheng,Betty Zhang, Crystal Li</td> |
| + | </tr> |
| + | <tr> |
| + | <td>Online sale</td> |
| + | <td>Vivian Huang</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | </section> |
| </section> | | </section> |
| + | |
| + | <section> |
| + | <h1 class="title">Support</h1> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">General support</h2> |
| + | <ul class="mark"> |
| + | <li><p>SubCat Academy思楷学术</p></li> |
| + | <li><p>ATLATL Innovation and Development Center ATLATL创新研发中心</p></li> |
| + | <li><p>Mr. Fan: Doctor of IPS CAS中科院上海巴斯德研究所</p></li> |
| + | <li><p>Families and friends of all team members</p></li> |
| + | </ul> |
| + | </section> |
| + | |
| + | <section> |
| + | <h2 class="title2 blue">Lab support</h2> |
| + | |
| + | <h3 class="title3">Laboratory and its instruments and equipments</h3> |
| + | <ul class="mark"> |
| + | <li><p>SubCat Academy思楷学术</p></li> |
| + | <li><p>ATLATL Innovation and Development Center ATLATL创新研发中心</p></li> |
| + | </ul> |
| + | |
| + | <h3 class="title3">Material</h3> |
| + | <ul class="mark"> |
| + | <li> |
| + | <p>GenScript Biotech Corporation 金斯瑞生物科技股份有限公司: All plasmids and primers used in our |
| + | experiment</p> |
| + | </li> |
| + | <li> |
| + | <p>TransGen Biotech Co., Ltd. 北京全式金生物技术有限公司: Loading buffers and markers in DNA gel |
| + | electrophoresis and SDS-PAGE</p> |
| + | </li> |
| + | <li><p>MACHEREY-NAGEL GmbH & Co. KG: DNA extraction kit</p></li> |
| + | <li><p>TIANGEN 天根生化科技(北京)有限公司: Plasmid extraction kit</p></li> |
| + | <li> |
| + | <p>Beijing Zoman Biotechnology Co., Ltd. 北京庄盟国际生物基因科技有限公司:Preparation of DH5α and BL21 (DE3) |
| + | chemically competent cells </p> |
| + | </li> |
| + | <li> |
| + | <p>Takara Biomedical Technology (Beijing) Co., Ltd. China 宝日医生物技术(北京)有限公司: All enzymes and other |
| + | materials required for PCR</p> |
| + | </li> |
| + | <li> |
| + | <p>Sangon Biotech (Shanghai) Co., Ltd. 生工生物工程(上海)股份有限公司: SDS-PAGE running buffer, TAE in DNA gel |
| + | electrophoresis, agarose and substances to prepare LB and MRS medium</p> |
| + | </li> |
| + | </ul> |
| + | |
| + | <h3 class="title3">Apparatus</h3> |
| + | <ul class="mark"> |
| + | <li> |
| + | <p>Bio-Rad 伯乐生命医学产品(上海)有限公司:PCR thermal cycler and electroporation machine</p> |
| + | </li> |
| + | <li> |
| + | <p>Shanghai Longyue Instrument Equipment Co., Ltd. 上海龙悦实验仪器有限公司: Shaker</p> |
| + | </li> |
| + | <li> |
| + | <p>Beijing Labgic Technology Co., Ltd. 北京兰杰柯科技有限公司: Micro-centrifuge tubes</p> |
| + | </li> |
| + | <li> |
| + | <p>WIX Technology Beijing Co., Ltd. 韦克斯科技(北京)有限公司: Power supply of DNA gel electrophoresis and |
| + | SDS-PAGE</p> |
| + | </li> |
| + | <li> |
| + | <p>Eppendorf 德国艾本德股份公司: Centrifuge</p> |
| + | </li> |
| + | </ul> |
| + | </section> |
| + | </section> |
| + | |
| + | <section> |
| + | <h1 class="title">Special Thanks</h1> |
| + | <ul class="mark"> |
| + | <li><p>Dr. Aifang Li, director of pediatrics at Hebei Yangyuan People's Hospital</p></li> |
| + | <li><p>Prof. Zhenggang Han, who holds a doctor's degree from the Chinese Academy of Sciences and a |
| + | master's degree in biology from Lubeck University in Germany</p></li> |
| + | <li><p>Mr. Zhang, a corporate strategy consultant from a large consulting firm</p></li> |
| + | <li><p>2021 iGEM teams: <b>Aseeker</b> and <b>Neuron Target</b></p></li> |
| + | <li><p>Tianlin Street Community Cultural Activity Center</p></li> |
| + | <li><p>Barun Shopping Plaza 八润.幻月城</p></li> |
| + | </ul> |
| + | </section> |
| + | |
| </section> | | </section> |
| </div> | | </div> |
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| <div class="content"> | | <div class="content"> |
| <hr> | | <hr> |
− | <span>Email us at: zhixuansong1022@qq.com</span> | + | <span>Email us at: zhixuansong1022@qq.com; 1956461994@qq.com</span> |
| <span>Search‘EV71terminator’ in Wechat to find more about us</span> | | <span>Search‘EV71terminator’ in Wechat to find more about us</span> |
| </div> | | </div> |