Log of July
2021/07/14
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Order plasmids.
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Order primers.
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Order commercial kits.
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Order reagents and apparatuses.
2021/07/28
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Finalize our project and main tasks.
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PCR.
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LB medium preparation.
2021/07/29
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OE PCR.
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Restriction enzyme double digestion.
2021/07/30
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Restriction enzyme double digestion.
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ClonExpress ligation reaction.
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Plasmid transformation.
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Construct plasmid profile(pGEX-6P-1-VP1&pGEX-6P-1-VP1-LTB)
2021/07/31
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Incubation of bacteria (in shaker).
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MRS medium preparation.
Log of August
2021/08/01
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Plasmid extraction.
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Restriction enzyme double digestion.
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Plasmid transformation.
2021/08/02
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Analysis of sequencing results.
2021/08/03
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SDS-PAGE(BL21chemically competent cells)
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Western Blot
2021/08/04
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SDS-PAGE(ATCC 334 chemically competent cells)
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Western Blot
