Team:Shanghai Metropolis/Attributions

Wet Lab

Experiment

PCR Amy Qu, Vannie Zhang
OE PCR Momo Wang, Jason Bi
Agarose Gel Electrophoresis Momo Wang
DNA Gel Extraction Jason Bi, Amy Qu
Restriction enzyme double digestion Yvonne Song, Amy Qu, Jimmy Jin, Vannie Zhang
Clonexpress Ligation reaction Amy Qu, Waylon Yang
Plasmid transformation Yang Zhou, Paul Niu
Plasmid extraction Yvonne Song, Momo Wang, Jason Bi
Protein detection Yvonne Song, Amy Qu, Vannie Zhang, Momo Wang
Medium preparation All of us
Wiki text Yvonne Song, Momo Wang, Amy Qu

Safety

Laboratory safety Jason Bi, Paul Niu, Momo Wang
Material safety Amy Qu
Application safety Yvonne Song, Vannie Zhang, Jimmy Jin, Waylon Yang
Wiki text All of us

Notebook

Daily record Yvonne Song, Amy Qu
Wiki text Yvonne Song, Amy Qu

Project description

Inspiration Yvonne Song, Amy Qu, Jimmy Jin
Background Momo Wang, Yvonne Song
General concept Momo Wang, Yvonne Song

Presentation

Background Amy Qu
General concept Jason Bi
Detailed process Momo Wang
Experiment results Yvonne Song

Dry Lab

Human practice

Research Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Jacky Cai, Rachel Zhou
Public survey Kevin Zou, Jacky Cai, Jason Xie,Sun Han, Vivian Huang, Betty Zhang, Rachel Zhou
Street interview Jason Xie, Kevin Zou, Carl Yuan, Rachel Zhou, Lee, Vivian Huang, Betty Zhang, Jacky Cai
Expert interview Jacky Cai, Rachel Zhou, Betty Zhang, Carl Yuan, Jacky Cai
Business plan Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Rachel Zhou, Lee,Betty Zhang, Jacky Cai
Collaboration All of us
Wiki text Lee, Rachel Zhou,Betty Zhang, Jason Xie, Carl Yuan, Jacky Cai, Rachel Zhou, Sun Han, Vivian Huang

Design

Logo & Team name Jason Xie,Betty Zhang, Rachel Zhou
Peripheral product Jason Xie, Carl Yuan, Jacky Cai
Publicity material Rachel Zhou, Betty Zhang, Vivian Huang

Promotion video

Actors Carl Yuan,Sun Han,Heidi Sheng, Kevin Zou, Jacky Cai
Script Heidi Sheng, Crystal Li
Shoot & Clip Vivian Huang

Publicity

Official account Jason Xie, Betty Zhang
Charity Vivian Huang, Lee, Rachel Zhou,Heidi Sheng,Betty Zhang, Crystal Li
Online sale Vivian Huang

Support

General support

  • SubCat Academy思楷学术

  • ATLATL Innovation and Development Center ATLATL创新研发中心

  • Mr. Fan: Doctor of IPS CAS中科院上海巴斯德研究所

  • Families and friends of all team members

Lab support

Laboratory and its instruments and equipments

  • SubCat Academy思楷学术

  • ATLATL Innovation and Development Center ATLATL创新研发中心

Material

  • GenScript Biotech Corporation 金斯瑞生物科技股份有限公司: All plasmids and primers used in our experiment

  • TransGen Biotech Co., Ltd. 北京全式金生物技术有限公司: Loading buffers and markers in DNA gel electrophoresis and SDS-PAGE

  • MACHEREY-NAGEL GmbH & Co. KG: DNA extraction kit

  • TIANGEN 天根生化科技(北京)有限公司: Plasmid extraction kit

  • Beijing Zoman Biotechnology Co., Ltd. 北京庄盟国际生物基因科技有限公司:Preparation of DH5α and BL21 (DE3) chemically competent cells

  • Takara Biomedical Technology (Beijing) Co., Ltd. China 宝日医生物技术(北京)有限公司: All enzymes and other materials required for PCR

  • Sangon Biotech (Shanghai) Co., Ltd. 生工生物工程(上海)股份有限公司: SDS-PAGE running buffer, TAE in DNA gel electrophoresis, agarose and substances to prepare LB and MRS medium

Apparatus

  • Bio-Rad 伯乐生命医学产品(上海)有限公司:PCR thermal cycler and electroporation machine

  • Shanghai Longyue Instrument Equipment Co., Ltd. 上海龙悦实验仪器有限公司: Shaker

  • Beijing Labgic Technology Co., Ltd. 北京兰杰柯科技有限公司: Micro-centrifuge tubes

  • WIX Technology Beijing Co., Ltd. 韦克斯科技(北京)有限公司: Power supply of DNA gel electrophoresis and SDS-PAGE

  • Eppendorf 德国艾本德股份公司: Centrifuge

Special Thanks

  • Dr. Aifang Li, director of pediatrics at Hebei Yangyuan People's Hospital

  • Prof. Zhenggang Han, who holds a doctor's degree from the Chinese Academy of Sciences and a master's degree in biology from Lubeck University in Germany

  • Mr. Zhang, a corporate strategy consultant from a large consulting firm

  • -2021 iGEM teams: Aseeker (Shanghai_United), Neuron Target (Nanjing_high_school) and FlavoneLabRouse (Shanghai_HS_United)

  • Tianlin Street Community Cultural Activity Center

  • Barun Shopping Plaza 八润.幻月城