|
|
| Line 21: |
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| | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Team">Team Members</a> | | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Team">Team Members</a> |
| | </li> | | </li> |
| − | <li class="child-item active"> | + | <li class="child-item"> |
| | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Attributions">Attributions</a> | | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Attributions">Attributions</a> |
| | </li> | | </li> |
| − | <li class="child-item"> | + | <li class="child-item active"> |
| | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Collaborations">Collaboration</a> | | <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Collaborations">Collaboration</a> |
| | </li> | | </li> |
| Line 121: |
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| | <div class="page-header"> | | <div class="page-header"> |
| | <img src="https://static.igem.org/mediawiki/2021/d/d2/T--Shanghai_Metropolis--bg-1.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2021/d/d2/T--Shanghai_Metropolis--bg-1.png" alt=""> |
| − | <h1>Attributions</h1> | + | <h1>Collaboration</h1> |
| | </div> | | </div> |
| | | | |
| Line 128: |
Line 128: |
| | <section class="article p-t-54 p-b-54"> | | <section class="article p-t-54 p-b-54"> |
| | <section> | | <section> |
| − | <h1 class="title">Wet Lab</h1> | + | <h1 class="title">1- Aseeker</h1> |
| | + | <img class="left" src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_Metropolis--img_collaborations_1.jpg" alt="" style="width: 360px"> |
| | + | <p>Our team have Collaborated with another iGEM team named Aseeker. Our collaboration is mainly through |
| | + | mutual understanding of each other's content and mutual advice to promote the process of both project. |
| | + | The central theme of the team's research is arsenic. Their research focuses on making dipsticks to |
| | + | detect arsenic and hopes to make the final product commercially available. They told us arsenic |
| | + | compounds, which are harmful to humans, have long been a problem for biological science. Testing for |
| | + | arsenic has become a central goal. In addition, they also explained their team's name: As is the |
| | + | chemical symbol for arsenic, while the name Ark stands for ark and hope. They are working on using |
| | + | genetic technology to create sensors based on e. coli to detect arsenic.</p> |
| | | | |
| − | <section> | + | <div class="half m-t-18 m-b-18"> |
| − | <h2 class="title2 blue">Experiment</h2>
| + | <div class="left"> |
| − | <table class="m-t-18 w-384">
| + | <img src="https://static.igem.org/mediawiki/2021/e/e7/T--Shanghai_Metropolis--img_collaborations_2.jpg" alt="" |
| − | <tbody>
| + | style="height: 284px; width: auto"> |
| − | <tr> | + | </div> |
| − | <td>PCR</td>
| + | <div class="right" style="text-align: right"> |
| − | <td>Amy Qu, Vannie Zhang</td>
| + | <img src="https://static.igem.org/mediawiki/2021/a/a3/T--Shanghai_Metropolis--img_collaborations_3.jpg" alt="" |
| − | </tr>
| + | style="height: 284px; width: auto"> |
| − | <tr>
| + | </div> |
| − | <td>OE PCR</td>
| + | </div> |
| − | <td>Momo Wang, Jason Bi</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Agarose Gel Electrophoresis</td>
| + | |
| − | <td>Momo Wang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>DNA Gel Extraction</td>
| + | |
| − | <td>Jason Bi, Amy Qu</td>
| + | |
| − | </tr> | + | |
| − | <tr>
| + | |
| − | <td>Restriction enzyme double digestion</td>
| + | |
| − | <td>Yvonne Song, Amy Qu, Jim Jin, Vannie Zhang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Clonexpress Ligation reaction</td>
| + | |
| − | <td>Amy Qu, Waylon Yang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Plasmid transformation</td>
| + | |
| − | <td>Yang Zhou, Paul Niu</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Plasmid extraction</td>
| + | |
| − | <td>Yvonne Song, Momo Wang, Jason Bi</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Protein detection</td>
| + | |
| − | <td>Yvonne Song, Amy Qu, Vannie Zhang, Momo Wang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Medium preparation</td>
| + | |
| − | <td>All of us</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Wiki text</td>
| + | |
| − | <td>Yvonne Song, Momo Wang, Amy Qu</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table> | + | |
| − | </section> | + | |
| | | | |
| − | <section> | + | <p>During the whole discussion, EV71terminator and Aseeker illustrated the challenges and effective methods |
| − | <h2 class="title2 blue">Safety</h2> | + | we have met in the research and human practice. After being informed of the fact that the secondary |
| − | <table class="m-t-18 w-384"> | + | product implies a potential business value, that is, the by-product of the bacteria probably is cable of |
| − | <tbody>
| + | eliminating the residual arsenic, whose accumulation is harmful for human body. We consulted whether |
| − | <tr>
| + | Aseeker would devote more time and financial resources into the R&D of the product, so as to gain some |
| − | <td>Laboratory safety</td>
| + | informative suggestions that might be helpful in future determination. It turned out that Aseeker is |
| − | <td>Jason Bi, Paul Niu, Momo Wang</td>
| + | intended to focus more on their main product. Moreover, EV71terminator also offered us experiences |
| − | </tr>
| + | related to human practice, such as field research, interview of the authority and charity bazaar. From |
| − | <tr>
| + | the discussion, we were exposed to several new points of view, and able to increase the efficiency in |
| − | <td>Material safety</td>
| + | future practice. </p> |
| − | <td>Amy Qu</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Application safety</td>
| + | |
| − | <td>Yvonne Song, Vannie Zhang, Jim Jin, Waylon Yang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Wiki text</td>
| + | |
| − | <td>All of us</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table> | + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h2 class="title2 blue">Notebook</h2> | + | |
| − | <table class="m-t-18 w-384">
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Daily record</td>
| + | |
| − | <td>Yvonne Song, Amy Qu</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Wiki text</td>
| + | |
| − | <td>Yvonne Song, Amy Qu</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table> | + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h2 class="title2 blue">Project description</h2> | + | |
| − | <table class="m-t-18 w-384"> | + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Inspiration</td>
| + | |
| − | <td>Yvonne Song, Amy Qu, Jim Jin</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Background</td>
| + | |
| − | <td>Momo Wang, Yvonne Song</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>General concept</td>
| + | |
| − | <td>Momo Wang, Yvonne Song</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table> | + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h2 class="title2 blue">Presentation</h2>
| + | |
| − | <table class="m-t-18 w-384">
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Background</td>
| + | |
| − | <td>Amy Qu</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>General concept</td>
| + | |
| − | <td>Jason Bi</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Detailed process</td>
| + | |
| − | <td>Momo Wang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Experiment results</td>
| + | |
| − | <td>Yvonne Song</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | </section>
| + | |
| | </section> | | </section> |
| | | | |
| | <section> | | <section> |
| − | <h1 class="title">Dry Lab</h1> | + | <h1 class="title">2- Neuron Target</h1> |
| − | <section> | + | <img class="left m-b-18" src="https://static.igem.org/mediawiki/2021/0/06/T--Shanghai_Metropolis--img_collaborations_4.jpg" alt="" style="width: 360px"> |
| − | <h2 class="title2 blue">Human practice</h2>
| + | <p>The second Chinese team that we collaborated with was from Nanjing, and their name is Neuron Target. The |
| − | <table class="m-t-18 w-384">
| + | subject is also of medical interest, and the product is a compound that can be found to address the |
| − | <tbody>
| + | inflammatory response caused by activated microglia.</p> |
| − | <tr>
| + | <img class="left m-t-18 m-b-18" src="https://static.igem.org/mediawiki/2021/6/6b/T--Shanghai_Metropolis--img_collaborations_5.png" alt=""> |
| − | <td>Research</td>
| + | <p>We were fortunate to participate in the presentation of Neuron Target's team. They showed us what they |
| − | <td>Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Jacky Cai, Rachel Zhou</td>
| + | were working on and what they were aiming for. At the same time, the team also showed us the daily life |
| − | </tr>
| + | of the team lab. We got to know each other, and discussed many topics such as product development and |
| − | <tr>
| + | promotion, cooperation between dry and wet teams, etc. Since our research direction is related to |
| − | <td>Public survey</td>
| + | medicine, the audience will be similar. By taking this opportunity, we learned a lot of knowledge about |
| − | <td>Kevin Zou, Jacky Cai, Jason Xie，Sun Han, Vivian Huang, Betty Zhang, Rachel Zhou</td>
| + | business promotion from each other. In the end, we paid attention to each other's propaganda platform, |
| − | </tr>
| + | and hoped to carry out further cooperation in the future.</p> |
| − | <tr>
| + | |
| − | <td>Street interview</td>
| + | |
| − | <td>Jason Xie, Kevin Zou, Carl Yuan, Rachel Zhou, Lee, Vivian Huang, Betty Zhang, Jacky Cai</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Expert interview</td>
| + | |
| − | <td>Jacky Cai, Rachel Zhou, Betty Zhang, Carl Yuan, Jacky Cai</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Business plan</td>
| + | |
| − | <td>Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Rachel Zhou, Lee，Betty Zhang, Jacky Cai</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Collaboration</td>
| + | |
| − | <td>All of us</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Wiki text</td>
| + | |
| − | <td>Lee, Rachel Zhou，Betty Zhang, Jason Xie, Carl Yuan, Jacky Cai, Rachel Zhou, Sun Han, Vivian
| + | |
| − | Huang
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h2 class="title2 blue">Design</h2>
| + | |
| − | <table class="m-t-18 w-384">
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Logo & Team name</td>
| + | |
| − | <td>Jason Xie，Betty Zhang, Rachel Zhou</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Peripheral product</td>
| + | |
| − | <td>Jason Xie, Carl Yuan, Jacky Cai</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Publicity material</td>
| + | |
| − | <td>Rachel Zhou, Betty Zhang, Vivian Huang</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table> | + | |
| − | </section> | + | |
| − | | + | |
| − | <section>
| + | |
| − | <h2 class="title2 blue">Promotion video</h2>
| + | |
| − | <table class="m-t-18 w-384">
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Actors</td>
| + | |
| − | <td>Carl Yuan，Sun Han，Heidi Sheng, Kevin Zou, Jacky Cai</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Script</td>
| + | |
| − | <td>Heidi Sheng, Crystal Li</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Shoot & Clip</td>
| + | |
| − | <td>Vivian Huang</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h2 class="title2 blue">Publicity</h2>
| + | |
| − | <table class="m-t-18 w-384">
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Official account</td>
| + | |
| − | <td>Jason Xie, Betty Zhang</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Charity</td>
| + | |
| − | <td>Vivian Huang, Lee, Rachel Zhou，Heidi Sheng，Betty Zhang, Crystal Li</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Online sale</td>
| + | |
| − | <td>Vivian Huang</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table> | + | |
| − | </section>
| + | |
| | </section> | | </section> |
| − |
| |
| − | <section>
| |
| − | <h1 class="title">Support</h1>
| |
| − |
| |
| − | <section>
| |
| − | <h2 class="title2 blue">General support</h2>
| |
| − | <ul class="mark">
| |
| − | <li><p>SubCat Academy思楷学术</p></li>
| |
| − | <li><p>ATLATL Innovation and Development Center ATLATL创新研发中心</p></li>
| |
| − | <li><p>Mr. Fan: Doctor of IPS CAS中科院上海巴斯德研究所</p></li>
| |
| − | <li><p>Families and friends of all team members</p></li>
| |
| − | </ul>
| |
| − | </section>
| |
| − |
| |
| − | <section>
| |
| − | <h2 class="title2 blue">Lab support</h2>
| |
| − |
| |
| − | <h3 class="title3">Laboratory and its instruments and equipments</h3>
| |
| − | <ul class="mark">
| |
| − | <li><p>SubCat Academy思楷学术</p></li>
| |
| − | <li><p>ATLATL Innovation and Development Center ATLATL创新研发中心</p></li>
| |
| − | </ul>
| |
| − |
| |
| − | <h3 class="title3">Material</h3>
| |
| − | <ul class="mark">
| |
| − | <li>
| |
| − | <p>GenScript Biotech Corporation 金斯瑞生物科技股份有限公司: All plasmids and primers used in our
| |
| − | experiment</p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>TransGen Biotech Co., Ltd. 北京全式金生物技术有限公司: Loading buffers and markers in DNA gel
| |
| − | electrophoresis and SDS-PAGE</p>
| |
| − | </li>
| |
| − | <li><p>MACHEREY-NAGEL GmbH & Co. KG: DNA extraction kit</p></li>
| |
| − | <li><p>TIANGEN 天根生化科技（北京）有限公司: Plasmid extraction kit</p></li>
| |
| − | <li>
| |
| − | <p>Beijing Zoman Biotechnology Co., Ltd. 北京庄盟国际生物基因科技有限公司：Preparation of DH5α and BL21 (DE3)
| |
| − | chemically competent cells </p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>Takara Biomedical Technology (Beijing) Co., Ltd. China 宝日医生物技术（北京）有限公司: All enzymes and other
| |
| − | materials required for PCR</p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>Sangon Biotech (Shanghai) Co., Ltd. 生工生物工程（上海）股份有限公司: SDS-PAGE running buffer, TAE in DNA gel
| |
| − | electrophoresis, agarose and substances to prepare LB and MRS medium</p>
| |
| − | </li>
| |
| − | </ul>
| |
| − |
| |
| − | <h3 class="title3">Apparatus</h3>
| |
| − | <ul class="mark">
| |
| − | <li>
| |
| − | <p>Bio-Rad 伯乐生命医学产品（上海）有限公司:PCR thermal cycler and electroporation machine</p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>Shanghai Longyue Instrument Equipment Co., Ltd. 上海龙悦实验仪器有限公司: Shaker</p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>Beijing Labgic Technology Co., Ltd. 北京兰杰柯科技有限公司: Micro-centrifuge tubes</p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>WIX Technology Beijing Co., Ltd. 韦克斯科技（北京）有限公司: Power supply of DNA gel electrophoresis and
| |
| − | SDS-PAGE</p>
| |
| − | </li>
| |
| − | <li>
| |
| − | <p>Eppendorf 德国艾本德股份公司: Centrifuge</p>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </section>
| |
| − | </section>
| |
| − |
| |
| − | <section>
| |
| − | <h1 class="title">Special Thanks</h1>
| |
| − | <ul class="mark">
| |
| − | <li><p>Dr. Aifang Li, director of pediatrics at Hebei Yangyuan People's Hospital</p></li>
| |
| − | <li><p>Prof. Zhenggang Han, who holds a doctor's degree from the Chinese Academy of Sciences and a
| |
| − | master's degree in biology from Lubeck University in Germany</p></li>
| |
| − | <li><p>Mr. Zhang, a corporate strategy consultant from a large consulting firm</p></li>
| |
| − | <li><p>2021 iGEM teams: <b>Aseeker</b> and <b>Neuron Target</b></p></li>
| |
| − | <li><p>Tianlin Street Community Cultural Activity Center</p></li>
| |
| − | <li><p>Barun Shopping Plaza 八润.幻月城</p></li>
| |
| − | </ul>
| |
| − | </section>
| |
| − |
| |
| | </section> | | </section> |
| | </div> | | </div> |
