Difference between revisions of "Team:Shanghai Metropolis/Attributions"

 
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                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Team">Team Members</a>
 
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Team">Team Members</a>
 
                         </li>
 
                         </li>
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                         <li class="child-item">
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                         <li class="child-item active">
 
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Attributions">Attributions</a>
 
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Attributions">Attributions</a>
 
                         </li>
 
                         </li>
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                         <li class="child-item active">
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                         <li class="child-item">
 
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Collaborations">Collaboration</a>
 
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Collaborations">Collaboration</a>
 
                         </li>
 
                         </li>
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             <!--Parts-->
 
             <!--Parts-->
 
             <li class="item">
 
             <li class="item">
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                 <a href="https://2021.igem.org/Team:Shanghai_Metropolis/PartsCollection">Parts</a>
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                 <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Parts">Parts</a>
 
                 <div class="child-nav">
 
                 <div class="child-nav">
 
                     <ul class="child-list">
 
                     <ul class="child-list">
 
                         <li class="child-item">
 
                         <li class="child-item">
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                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Parts_Collection">Parts
+
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Parts">Parts
 
                                 Collection</a>
 
                                 Collection</a>
 
                         </li>
 
                         </li>
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                         </li>
 
                         </li>
 
                         <li class="child-item">
 
                         <li class="child-item">
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                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Results">Result</a>
+
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Results">Results</a>
 
                         </li>
 
                         </li>
 
                         <li class="child-item">
 
                         <li class="child-item">
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                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/ProofOfConcept">Proof of Concept</a>
+
                             <a href="https://2021.igem.org/Team:Shanghai_Metropolis/Proof_Of_Concept">Proof of Concept</a>
 
                         </li>
 
                         </li>
 
                         <li class="child-item">
 
                         <li class="child-item">
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     <div class="page-header">
 
     <div class="page-header">
 
         <img src="https://static.igem.org/mediawiki/2021/d/d2/T--Shanghai_Metropolis--bg-1.png" alt="">
 
         <img src="https://static.igem.org/mediawiki/2021/d/d2/T--Shanghai_Metropolis--bg-1.png" alt="">
−
         <h1>Collaboration</h1>
+
         <h1>Attributions</h1>
 
     </div>
 
     </div>
  
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         <section class="article p-t-54 p-b-54">
 
         <section class="article p-t-54 p-b-54">
 
             <section>
 
             <section>
−
                 <h1 class="title">1- Aseeker</h1>
+
                 <h1 class="title">Wet Lab</h1>
−
                <img class="left" src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_Metropolis--img_collaborations_1.jpg" alt="" style="width: 360px">
+
−
                <p>Our team have Collaborated with another iGEM team named Aseeker. Our collaboration is mainly through
+
−
                    mutual understanding of each other's content and mutual advice to promote the process of both project.
+
−
                    The central theme of the team's research is arsenic. Their research focuses on making dipsticks to
+
−
                    detect arsenic and hopes to make the final product commercially available. They told us arsenic
+
−
                    compounds, which are harmful to humans, have long been a problem for biological science. Testing for
+
−
                    arsenic has become a central goal. In addition, they also explained their team's name: As is the
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−
                    chemical symbol for arsenic, while the name Ark stands for ark and hope. They are working on using
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−
                    genetic technology to create sensors based on e. coli to detect arsenic.</p>
+
  
−
                 <div class="half m-t-18 m-b-18">
+
                 <section>
−
                    <div class="left">
+
                    <h2 class="title2 blue">Experiment</h2>
−
                         <img src="https://static.igem.org/mediawiki/2021/e/e7/T--Shanghai_Metropolis--img_collaborations_2.jpg" alt=""
+
                    <table class="m-t-18 w-384">
−
                            style="height: 284px; width: auto">
+
                        <tbody>
−
                    </div>
+
                         <tr>
−
                    <div class="right" style="text-align: right">
+
                            <td>PCR</td>
−
                         <img src="https://static.igem.org/mediawiki/2021/a/a3/T--Shanghai_Metropolis--img_collaborations_3.jpg" alt=""
+
                            <td>Amy Qu, Vannie Zhang</td>
−
                            style="height: 284px; width: auto">
+
                        </tr>
−
                     </div>
+
                        <tr>
−
                 </div>
+
                            <td>OE PCR</td>
 +
                            <td>Momo Wang, Jason Bi</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Agarose Gel Electrophoresis</td>
 +
                            <td>Momo Wang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>DNA Gel Extraction</td>
 +
                            <td>Jason Bi, Amy Qu</td>
 +
                         </tr>
 +
                        <tr>
 +
                            <td>Restriction enzyme double digestion</td>
 +
                            <td>Yvonne Song, Amy Qu, Jimmy Jin, Vannie Zhang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Clonexpress Ligation reaction</td>
 +
                            <td>Amy Qu, Waylon Yang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Plasmid transformation</td>
 +
                            <td>Yang Zhou, Paul Niu</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Plasmid extraction</td>
 +
                            <td>Yvonne Song, Momo Wang, Jason Bi</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Protein detection</td>
 +
                            <td>Yvonne Song, Amy Qu, Vannie Zhang, Momo Wang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Medium preparation</td>
 +
                            <td>All of us</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Wiki text</td>
 +
                            <td>Yvonne Song, Momo Wang, Amy Qu</td>
 +
                        </tr>
 +
                        </tbody>
 +
                     </table>
 +
                 </section>
  
−
                 <p>During the whole discussion, EV71terminator and Aseeker illustrated the challenges and effective methods
+
                 <section>
−
                     we have met in the research and human practice. After being informed of the fact that the secondary
+
                     <h2 class="title2 blue">Safety</h2>
−
                     product implies a potential business value, that is, the by-product of the bacteria probably is cable of
+
                     <table class="m-t-18 w-384">
−
                     eliminating the residual arsenic, whose accumulation is harmful for human body. We consulted whether
+
                        <tbody>
−
                     Aseeker would devote more time and financial resources into the R&D of the product, so as to gain some
+
                        <tr>
−
                     informative suggestions that might be helpful in future determination. It turned out that Aseeker is
+
                            <td>Laboratory safety</td>
−
                     intended to focus more on their main product. Moreover, EV71terminator also offered us experiences
+
                            <td>Jason Bi, Paul Niu, Momo Wang</td>
−
                     related to human practice, such as field research, interview of the authority and charity bazaar. From
+
                        </tr>
−
                    the discussion, we were exposed to several new points of view, and able to increase the efficiency in
+
                        <tr>
−
                     future practice. </p>
+
                            <td>Material safety</td>
 +
                            <td>Amy Qu</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Application safety</td>
 +
                            <td>Yvonne Song, Vannie Zhang, Jimmy Jin, Waylon Yang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Wiki text</td>
 +
                            <td>All of us</td>
 +
                        </tr>
 +
                        </tbody>
 +
                     </table>
 +
                </section>
 +
 
 +
                <section>
 +
                     <h2 class="title2 blue">Notebook</h2>
 +
                    <table class="m-t-18 w-384">
 +
                        <tbody>
 +
                        <tr>
 +
                            <td>Daily record</td>
 +
                            <td>Yvonne Song, Amy Qu</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Wiki text</td>
 +
                            <td>Yvonne Song, Amy Qu</td>
 +
                        </tr>
 +
                        </tbody>
 +
                     </table>
 +
                </section>
 +
 
 +
                <section>
 +
                     <h2 class="title2 blue">Project description</h2>
 +
                     <table class="m-t-18 w-384">
 +
                        <tbody>
 +
                        <tr>
 +
                            <td>Inspiration</td>
 +
                            <td>Yvonne Song, Amy Qu, Jimmy Jin</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Background</td>
 +
                            <td>Momo Wang, Yvonne Song</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>General concept</td>
 +
                            <td>Momo Wang, Yvonne Song</td>
 +
                        </tr>
 +
                        </tbody>
 +
                     </table>
 +
                </section>
 +
 
 +
                <section>
 +
                    <h2 class="title2 blue">Presentation</h2>
 +
                    <table class="m-t-18 w-384">
 +
                        <tbody>
 +
                        <tr>
 +
                            <td>Background</td>
 +
                            <td>Amy Qu</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>General concept</td>
 +
                            <td>Jason Bi</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Detailed process</td>
 +
                            <td>Momo Wang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Experiment results</td>
 +
                            <td>Yvonne Song</td>
 +
                        </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </section>
 
             </section>
 
             </section>
  
 
             <section>
 
             <section>
−
                 <h1 class="title">2- Neuron Target</h1>
+
                 <h1 class="title">Dry Lab</h1>
−
                 <img class="left m-b-18" src="https://static.igem.org/mediawiki/2021/0/06/T--Shanghai_Metropolis--img_collaborations_4.jpg" alt="" style="width: 360px">
+
                 <section>
−
                <p>The second Chinese team that we collaborated with was from Nanjing, and their name is Neuron Target. The
+
                    <h2 class="title2 blue">Human practice</h2>
−
                    subject is also of medical interest, and the product is a compound that can be found to address the
+
                    <table class="m-t-18 w-384">
−
                     inflammatory response caused by activated microglia.</p>
+
                        <tbody>
−
                 <img class="left m-t-18 m-b-18" src="https://static.igem.org/mediawiki/2021/6/6b/T--Shanghai_Metropolis--img_collaborations_5.png" alt="">
+
                        <tr>
−
                <p>We were fortunate to participate in the presentation of Neuron Target's team. They showed us what they
+
                            <td>Research</td>
−
                    were working on and what they were aiming for. At the same time, the team also showed us the daily life
+
                            <td>Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Jacky Cai, Rachel Zhou</td>
−
                    of the team lab. We got to know each other, and discussed many topics such as product development and
+
                        </tr>
−
                    promotion, cooperation between dry and wet teams, etc. Since our research direction is related to
+
                        <tr>
−
                    medicine, the audience will be similar. By taking this opportunity, we learned a lot of knowledge about
+
                            <td>Public survey</td>
−
                    business promotion from each other. In the end, we paid attention to each other's propaganda platform,
+
                            <td>Kevin Zou, Jacky Cai, Jason Xie，Sun Han, Vivian Huang, Betty Zhang, Rachel Zhou</td>
−
                     and hoped to carry out further cooperation in the future.</p>
+
                        </tr>
 +
                        <tr>
 +
                            <td>Street interview</td>
 +
                            <td>Jason Xie, Kevin Zou, Carl Yuan, Rachel Zhou, Lee, Vivian Huang, Betty Zhang, Jacky Cai</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Expert interview</td>
 +
                            <td>Jacky Cai, Rachel Zhou, Betty Zhang, Carl Yuan, Jacky Cai</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Business plan</td>
 +
                            <td>Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Rachel Zhou, Lee，Betty Zhang, Jacky Cai</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Collaboration</td>
 +
                            <td>All of us</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Wiki text</td>
 +
                            <td>Lee, Rachel Zhou，Betty Zhang, Jason Xie, Carl Yuan, Jacky Cai, Rachel Zhou, Sun Han, Vivian
 +
                                Huang
 +
                            </td>
 +
                        </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </section>
 +
 
 +
                <section>
 +
                    <h2 class="title2 blue">Design</h2>
 +
                    <table class="m-t-18 w-384">
 +
                        <tbody>
 +
                        <tr>
 +
                            <td>Logo & Team name</td>
 +
                            <td>Jason Xie，Betty Zhang, Rachel Zhou</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Peripheral product</td>
 +
                            <td>Jason Xie, Carl Yuan, Jacky Cai</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Publicity material</td>
 +
                            <td>Rachel Zhou, Betty Zhang, Vivian Huang</td>
 +
                        </tr>
 +
                        </tbody>
 +
                     </table>
 +
                 </section>
 +
 
 +
                <section>
 +
                    <h2 class="title2 blue">Promotion video</h2>
 +
                    <table class="m-t-18 w-384">
 +
                        <tbody>
 +
                        <tr>
 +
                            <td>Actors</td>
 +
                            <td>Carl Yuan，Sun Han，Heidi Sheng, Kevin Zou, Jacky Cai</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Script</td>
 +
                            <td>Heidi Sheng, Crystal Li</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Shoot & Clip</td>
 +
                            <td>Vivian Huang</td>
 +
                        </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </section>
 +
 
 +
                <section>
 +
                    <h2 class="title2 blue">Publicity</h2>
 +
                    <table class="m-t-18 w-384">
 +
                        <tbody>
 +
                        <tr>
 +
                            <td>Official account</td>
 +
                            <td>Jason Xie, Betty Zhang</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Charity</td>
 +
                            <td>Vivian Huang, Lee, Rachel Zhou，Heidi Sheng，Betty Zhang, Crystal Li</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>Online sale</td>
 +
                            <td>Vivian Huang</td>
 +
                        </tr>
 +
                        </tbody>
 +
                     </table>
 +
                </section>
 
             </section>
 
             </section>
 +
 +
            <section>
 +
                <h1 class="title">Support</h1>
 +
 +
                <section>
 +
                    <h2 class="title2 blue">General support</h2>
 +
                    <ul class="mark">
 +
                        <li><p>SubCat Academy思楷学术</p></li>
 +
                        <li><p>ATLATL Innovation and Development Center ATLATL创新研发中心</p></li>
 +
                        <li><p>Mr. Fan: Doctor of IPS CAS中科院上海巴斯德研究所</p></li>
 +
                        <li><p>Families and friends of all team members</p></li>
 +
                    </ul>
 +
                </section>
 +
 +
                <section>
 +
                    <h2 class="title2 blue">Lab support</h2>
 +
 +
                    <h3 class="title3">Laboratory and its instruments and equipments</h3>
 +
                    <ul class="mark">
 +
                        <li><p>SubCat Academy思楷学术</p></li>
 +
                        <li><p>ATLATL Innovation and Development Center ATLATL创新研发中心</p></li>
 +
                    </ul>
 +
 +
                    <h3 class="title3">Material</h3>
 +
                    <ul class="mark">
 +
                        <li>
 +
                            <p>GenScript Biotech Corporation 金斯瑞生物科技股份有限公司: All plasmids and primers used in our
 +
                                experiment</p>
 +
                        </li>
 +
                        <li>
 +
                            <p>TransGen Biotech Co., Ltd. 北京全式金生物技术有限公司: Loading buffers and markers in DNA gel
 +
                                electrophoresis and SDS-PAGE</p>
 +
                        </li>
 +
                        <li><p>MACHEREY-NAGEL GmbH & Co. KG: DNA extraction kit</p></li>
 +
                        <li><p>TIANGEN 天根生化科技（北京）有限公司: Plasmid extraction kit</p></li>
 +
                        <li>
 +
                            <p>Beijing Zoman Biotechnology Co., Ltd. 北京庄盟国际生物基因科技有限公司：Preparation of DH5α and BL21 (DE3)
 +
                                chemically competent cells </p>
 +
                        </li>
 +
                        <li>
 +
                            <p>Takara Biomedical Technology (Beijing) Co., Ltd. China 宝日医生物技术（北京）有限公司: All enzymes and other
 +
                                materials required for PCR</p>
 +
                        </li>
 +
                        <li>
 +
                            <p>Sangon Biotech (Shanghai) Co., Ltd. 生工生物工程（上海）股份有限公司: SDS-PAGE running buffer, TAE in DNA gel
 +
                                electrophoresis, agarose and substances to prepare LB and MRS medium</p>
 +
                        </li>
 +
                    </ul>
 +
 +
                    <h3 class="title3">Apparatus</h3>
 +
                    <ul class="mark">
 +
                        <li>
 +
                            <p>Bio-Rad 伯乐生命医学产品（上海）有限公司:PCR thermal cycler and electroporation machine</p>
 +
                        </li>
 +
                        <li>
 +
                            <p>Shanghai Longyue Instrument Equipment Co., Ltd. 上海龙悦实验仪器有限公司: Shaker</p>
 +
                        </li>
 +
                        <li>
 +
                            <p>Beijing Labgic Technology Co., Ltd. 北京兰杰柯科技有限公司: Micro-centrifuge tubes</p>
 +
                        </li>
 +
                        <li>
 +
                            <p>WIX Technology Beijing Co., Ltd. 韦克斯科技（北京）有限公司: Power supply of DNA gel electrophoresis and
 +
                                SDS-PAGE</p>
 +
                        </li>
 +
                        <li>
 +
                            <p>Eppendorf 德国艾本德股份公司: Centrifuge</p>
 +
                        </li>
 +
                    </ul>
 +
                </section>
 +
            </section>
 +
 +
            <section>
 +
                <h1 class="title">Special Thanks</h1>
 +
                <ul class="mark">
 +
                    <li><p>Dr. Aifang Li, director of pediatrics at Hebei Yangyuan People's Hospital</p></li>
 +
                    <li><p>Prof. Zhenggang Han, who holds a doctor's degree from the Chinese Academy of Sciences and a
 +
                        master's degree in biology from Lubeck University in Germany</p></li>
 +
                    <li><p>Mr. Zhang, a corporate strategy consultant from a large consulting firm</p></li>
 +
                    <li>
 +
                        <p>
 +
                            -2021 iGEM teams: <b>Aseeker (Shanghai_United)</b>, <b>Neuron Target (Nanjing_high_school)</b>
 +
                            and <b>FlavoneLabRouse (Shanghai_HS_United)</b>
 +
                        </p>
 +
                    </li>
 +
                    <li><p>Tianlin Street Community Cultural Activity Center</p></li>
 +
                    <li><p>Barun Shopping Plaza 八润.幻月城</p></li>
 +
                </ul>
 +
            </section>
 +
 
         </section>
 
         </section>
 
     </div>
 
     </div>
Line 184: Line 463:
 
         <div class="content">
 
         <div class="content">
 
             <hr>
 
             <hr>
−
             <span>Email us at: zhixuansong1022@qq.com</span>
+
             <span>Email us at: zhixuansong1022@qq.com; 1956461994@qq.com</span>
 
             <span>Search‘EV71terminator’ in Wechat to find more about us</span>
 
             <span>Search‘EV71terminator’ in Wechat to find more about us</span>
 
         </div>
 
         </div>

Latest revision as of 12:37, 20 October 2021

Attributions

Wet Lab

Experiment

PCR Amy Qu, Vannie Zhang
OE PCR Momo Wang, Jason Bi
Agarose Gel Electrophoresis Momo Wang
DNA Gel Extraction Jason Bi, Amy Qu
Restriction enzyme double digestion Yvonne Song, Amy Qu, Jimmy Jin, Vannie Zhang
Clonexpress Ligation reaction Amy Qu, Waylon Yang
Plasmid transformation Yang Zhou, Paul Niu
Plasmid extraction Yvonne Song, Momo Wang, Jason Bi
Protein detection Yvonne Song, Amy Qu, Vannie Zhang, Momo Wang
Medium preparation All of us
Wiki text Yvonne Song, Momo Wang, Amy Qu

Safety

Laboratory safety Jason Bi, Paul Niu, Momo Wang
Material safety Amy Qu
Application safety Yvonne Song, Vannie Zhang, Jimmy Jin, Waylon Yang
Wiki text All of us

Notebook

Daily record Yvonne Song, Amy Qu
Wiki text Yvonne Song, Amy Qu

Project description

Inspiration Yvonne Song, Amy Qu, Jimmy Jin
Background Momo Wang, Yvonne Song
General concept Momo Wang, Yvonne Song

Presentation

Background Amy Qu
General concept Jason Bi
Detailed process Momo Wang
Experiment results Yvonne Song

Dry Lab

Human practice

Research Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Jacky Cai, Rachel Zhou
Public survey Kevin Zou, Jacky Cai, Jason Xie，Sun Han, Vivian Huang, Betty Zhang, Rachel Zhou
Street interview Jason Xie, Kevin Zou, Carl Yuan, Rachel Zhou, Lee, Vivian Huang, Betty Zhang, Jacky Cai
Expert interview Jacky Cai, Rachel Zhou, Betty Zhang, Carl Yuan, Jacky Cai
Business plan Jason Xie, Carl Yuan, Sun Han, Vivian Huang, Rachel Zhou, Lee，Betty Zhang, Jacky Cai
Collaboration All of us
Wiki text Lee, Rachel Zhou，Betty Zhang, Jason Xie, Carl Yuan, Jacky Cai, Rachel Zhou, Sun Han, Vivian Huang

Design

Logo & Team name Jason Xie，Betty Zhang, Rachel Zhou
Peripheral product Jason Xie, Carl Yuan, Jacky Cai
Publicity material Rachel Zhou, Betty Zhang, Vivian Huang

Promotion video

Actors Carl Yuan，Sun Han，Heidi Sheng, Kevin Zou, Jacky Cai
Script Heidi Sheng, Crystal Li
Shoot & Clip Vivian Huang

Publicity

Official account Jason Xie, Betty Zhang
Charity Vivian Huang, Lee, Rachel Zhou，Heidi Sheng，Betty Zhang, Crystal Li
Online sale Vivian Huang

Support

General support

  • SubCat Academy思楷学术

  • ATLATL Innovation and Development Center ATLATL创新研发中心

  • Mr. Fan: Doctor of IPS CAS中科院上海巴斯德研究所

  • Families and friends of all team members

Lab support

Laboratory and its instruments and equipments

  • SubCat Academy思楷学术

  • ATLATL Innovation and Development Center ATLATL创新研发中心

Material

  • GenScript Biotech Corporation 金斯瑞生物科技股份有限公司: All plasmids and primers used in our experiment

  • TransGen Biotech Co., Ltd. 北京全式金生物技术有限公司: Loading buffers and markers in DNA gel electrophoresis and SDS-PAGE

  • MACHEREY-NAGEL GmbH & Co. KG: DNA extraction kit

  • TIANGEN 天根生化科技（北京）有限公司: Plasmid extraction kit

  • Beijing Zoman Biotechnology Co., Ltd. 北京庄盟国际生物基因科技有限公司：Preparation of DH5α and BL21 (DE3) chemically competent cells

  • Takara Biomedical Technology (Beijing) Co., Ltd. China 宝日医生物技术（北京）有限公司: All enzymes and other materials required for PCR

  • Sangon Biotech (Shanghai) Co., Ltd. 生工生物工程（上海）股份有限公司: SDS-PAGE running buffer, TAE in DNA gel electrophoresis, agarose and substances to prepare LB and MRS medium

Apparatus

  • Bio-Rad 伯乐生命医学产品（上海）有限公司:PCR thermal cycler and electroporation machine

  • Shanghai Longyue Instrument Equipment Co., Ltd. 上海龙悦实验仪器有限公司: Shaker

  • Beijing Labgic Technology Co., Ltd. 北京兰杰柯科技有限公司: Micro-centrifuge tubes

  • WIX Technology Beijing Co., Ltd. 韦克斯科技（北京）有限公司: Power supply of DNA gel electrophoresis and SDS-PAGE

  • Eppendorf 德国艾本德股份公司: Centrifuge

Special Thanks

  • Dr. Aifang Li, director of pediatrics at Hebei Yangyuan People's Hospital

  • Prof. Zhenggang Han, who holds a doctor's degree from the Chinese Academy of Sciences and a master's degree in biology from Lubeck University in Germany

  • Mr. Zhang, a corporate strategy consultant from a large consulting firm

  • -2021 iGEM teams: Aseeker (Shanghai_United), Neuron Target (Nanjing_high_school) and FlavoneLabRouse (Shanghai_HS_United)

  • Tianlin Street Community Cultural Activity Center

  • Barun Shopping Plaza 八润.幻月城

Email us at: zhixuansong1022@qq.com; 1956461994@qq.com Search‘EV71terminator’ in Wechat to find more about us