For the usage of our constructs in the laboratory we used several plasmid backbones. The ones we were able to test were cloned using the GoldenBraid 2.0 or the Golden Gate Assembly method. The following table contains the plasmids that were used to clone and express the genetic constructs.
Table 1. Vectors that were used in the lab experiments.
| Name | Length (bp) | Resistance | Features |
| pDGB3_alpha1 | 6965 | Kanamycin | M13 fwd, pUC18/19 MCS, M13 rev, lac operator, lac promoter, CAP binding site, RB T-DNA repeat, pVS1 StaA, pVS1 RepA, pVS1 oriV, bom, high-copy-number ColE1/pMB1/pBR322/pUC ori, KanR, LB T-DNA repeat |
| pDGB3_alpha1R | 6969 | Kanamycin | CAP binding site, lac promoter, lac operator, M13 rev, pUC18/19 MCS, M13 fwd, RB T-DNA repeat, pVS1 StaA, pVS1 RepA, pVS1 oriV, bom, high-copy-number ColE1/pMB1/pBR322/pUC ori, KanR, LB T-DNA repeat |
| pDGB3_alpha2 | 6967 | Kanamycin | M13 fwd, pUC18/19 MCS, M13 rev, lac operator, lac promoter, CAP binding site, RB T-DNA repeat, pVS1 StaA, pVS1 RepA, pVS1 oriV, bom, high-copy-number ColE1/pMB1/pBR322/pUC ori, KanR, LB T-DNA repeat |
| pDGB3_omega1 | 7295 | Spectinomycin | M13 fwd, pUC18/19 MCS, M13 rev, lac operator, lac promoter, CAP binding site, RB T-DNA repeat, pVS1 StaA, pVS1 RepA, pVS1 oriV, bom, high-copy-number ColE1/pMB1/pBR322/pUC ori, SmR, LB T-DNA repeat |
| pBAD (Arabinose inducible) | 5111 | Kanamycin | 6xHis affinity tag, AraC transcriptional regulator, araBAD promoter, mRFP1, ssrA tag (LVA), rrnB T1 terminator, T7 terminator, primer binding site, Aminoglycoside Phosphotransferase, incP origin of transfer, rep101 |
| LacI Repressor (IPTG inducible promoter) | 6305 | Chloramphenicol | L4440 vector, forward primer, lac repressor encoded by lacI binding site, 6xHis affinity tag, rrnB T1 terminator, rrnB T2 terminator, pBR322 origin, forward primer, CmR, T7 Polymerase, LacI, lacI promoter |