Team:TAS Taipei/Parts

Description

PARTS COLLECTION



Description



The range of our part numbers is BBa_K3717001 ~ BBa_K3717016. Our parts collection includes new parts for three glycoside hydrolases: α-Galactosidase, α-N-Acetylgalactosaminidase, and Endo-β-Galactosidase, that cleave the terminal sugars of A and B antigens on red blood cells. These specific enzymes were chosen for their high efficiency on red blood cells. This collection includes both improved parts that we contributed to as well as new parts that we created where we added combinations of promoters, ribosome binding sites, and terminators to the sequences. To purify and use these synthesized proteins, we attached a 6x Histidine tag in all our sequence’s open reading frames through a flexible Glycine-Serine linker. Using our parts collection, one is able to purify enzymes that convert types A, B, and AB blood into universal O type blood through multiple pathways, eliminating patient donor incompatibility to alleviate the blood shortage issue.



Basic Parts



Part Number Part Name Diagram
BBa_K3717006 α-Galactosidase with N-Terminal 6x Histidine tag
BBa_K3717007 α-N-Acetylgalactosaminidase with N-Terminal 6x Histidine tag
BBa_K3717008 Endo-β-Galactosidase with N-Terminal 6x Histidine tag
BBa_K3717015 α-Galactosidase with C-Terminal 6x Histidine tag
BBa_K3717016 α-N-Acetylgalactosaminidase with C-Terminal 6x Histidine tag


Composite Parts



Part Number Part Name Diagram
BBa_K3717001 T7 + RBS + α-Galactosidase
BBa_K3717002 T7 + RBS + α-N-Acetylgalactosamindase
BBa_K3717014 T7 + RBS + Endo-β-Galactosidase
BBa_K3717003 K88 + α-Galactosidase
BBa_K3717004 K88 + α-N-Acetylgalactosamindase
BBa_K3717005 K88 + Endo-β-Galactosidase
BBa_K3717009 α-Galactosidase with T7 promoter, RBS, and N-Terminal 6x Histidine tag
BBa_K3717010 α-N-Acetylgalactosamindase with T7 promoter, RBS, and N-Terminal 6x Histidine tag
BBa_K3717011 Endo-β-Galactosidase with T7 promoter, RBS, and N-Terminal 6x Histidine tag
BBa_K3717012 α-Galactosidase with T7 promoter, RBS, and C-Terminal 6x Histidine tag
BBa_K3717013 α-N-Acetylgalactosamindase with T7 promoter, RBS, and C-Terminal 6x Histidine tag