Team:TAS Taipei/Parts
PARTS COLLECTION
Description
The range of our part numbers is BBa_K3717001 ~ BBa_K3717016. Our parts collection includes new parts for three glycoside hydrolases: α-Galactosidase, α-N-Acetylgalactosaminidase, and Endo-β-Galactosidase, that cleave the terminal sugars of A and B antigens on red blood cells. These specific enzymes were chosen for their high efficiency on red blood cells. This collection includes both improved parts that we contributed to as well as new parts that we created where we added combinations of promoters, ribosome binding sites, and terminators to the sequences. To purify and use these synthesized proteins, we attached a 6x Histidine tag in all our sequence’s open reading frames through a flexible Glycine-Serine linker. Using our parts collection, one is able to purify enzymes that convert types A, B, and AB blood into universal O type blood through multiple pathways, eliminating patient donor incompatibility to alleviate the blood shortage issue.
Basic Parts
| Part Number | Part Name | Diagram |
|---|---|---|
| BBa_K3717006 | α-Galactosidase with N-Terminal 6x Histidine tag |
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| BBa_K3717007 | α-N-Acetylgalactosaminidase with N-Terminal 6x Histidine tag |
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| BBa_K3717008 | Endo-β-Galactosidase with N-Terminal 6x Histidine tag |
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| BBa_K3717015 | α-Galactosidase with C-Terminal 6x Histidine tag |
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| BBa_K3717016 | α-N-Acetylgalactosaminidase with C-Terminal 6x Histidine tag |
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Composite Parts
| Part Number | Part Name | Diagram |
|---|---|---|
| BBa_K3717001 | T7 + RBS + α-Galactosidase |
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| BBa_K3717002 | T7 + RBS + α-N-Acetylgalactosamindase |
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| BBa_K3717014 | T7 + RBS + Endo-β-Galactosidase |
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| BBa_K3717003 | K88 + α-Galactosidase |
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| BBa_K3717004 | K88 + α-N-Acetylgalactosamindase |
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| BBa_K3717005 | K88 + Endo-β-Galactosidase |
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| BBa_K3717009 | α-Galactosidase with T7 promoter, RBS, and N-Terminal 6x Histidine tag |
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| BBa_K3717010 | α-N-Acetylgalactosamindase with T7 promoter, RBS, and N-Terminal 6x Histidine tag |
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| BBa_K3717011 | Endo-β-Galactosidase with T7 promoter, RBS, and N-Terminal 6x Histidine tag |
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| BBa_K3717012 | α-Galactosidase with T7 promoter, RBS, and C-Terminal 6x Histidine tag |
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| BBa_K3717013 | α-N-Acetylgalactosamindase with T7 promoter, RBS, and C-Terminal 6x Histidine tag |
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