Our team designed the SNAP tag and histidine tag combination plasmids which contains a tag on the fimbriae following the fimbrial amino acid leader sequence ath the N terminus. Sequencing of bacteria containing this tag verified that the design was effective and the tags were able to integrated into the native fimbriae without being cleaving during the process of protein translation and modification.

The Pittsburgh iGEM team's 2021 project has laid the foundation for expansion of an invaluable innovation that could start a worldwide change in access to colorectal cancer detection. Future teams have the ability to take the included protocols and expand upon them to complete the engineering process and begin the implementation steps.