Attributions

The Team

Wetlab – Isaac, Kendall, Ethan, Emma, Madhu, Angel

Project Design – Isaac, Kendall, Ethan, Emma, Madhu, Angel

Outreach – Madhu

Children's Book – Kendall, Emma, Ethan, Madhu, Angel

Wiki Coding and Design – Emma, Madhu, Angel, Kendall

Wiki Content – Kendall, Ethan, Emma, Madhu, Angel

DNA Design – Angel, Ethan

Ordering Parts – Angel

Experimental Execution - Isaac, Kendall, Ethan, Emma, Madhu, Angel

Promotional video – Kendall, Ethan, Emma

Presentation video – Kendall

Advisors

We are extremely appreciative of all the feedback and support our wonderful mentors have provided us throughout this process. We extend our thanks to all four of them for attending our weekly meetings, providing experimental guidance and feedback, and granting us this opportunity to compete.

Dr. W. Seth Childers facilitated our meetings to ensure all team members and mentors were present and involved in the experimental design process. He offered consistent advice on how to better our experimental design and was consistenly available to answer any questions we had. He provided us with materials from his lab and allowed us to perform experiments there as well.

Dr. Alex Deiters provided consistent insight and advice on our project design, experimental results, and human practice initiatives. He also provided feedback on wiki design and as well as assisting us in the interpretation of lab results.

Dr. Jason Lohmueller provided consistent insight and advice on our project design, experimental results, and human practice initiatives. He also assisted with troubleshooting issues and interpreting lab results.

Dr. Sanjeev Shroff managed the budget and finances of our team as well as providing insightful advice on our experimental protocols, outreach initiatives, and collaboration efforts.

Lab Support

We thank Dr. Natasa Miskov-Zivanov for generously allowing us her lab space at the Center for Biotechnology and Bioengineering at the University of Pittsburgh to conduct our research.

We also send a special thanks to Dr. Dave Gau for providing reagents and resources for our experiments as well as allowing us access to lab equipment such as the spectrophotometer and autoclave.

Interviews

We want to especially thank all the experts that have taken the time out of their schedules to provide their input on our project. Their advice has been a valuable resource in determining the direction and design of our project.

Dr Robert Shanks, PhD. - Assistant Professor of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine

Dr Robert Schoen, MD MPH -Chief, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh Medical Center

Special thanks to Alison Morris and Leah Russell for providing us guidance and contacts for our project via email.

Project Start and Team Training

Team members of the Pittsburgh iGEM Team were selected during the beginning of April. Our first zoom meeting took place at the end of April, and we began brainstorming project ideas in May. By the end of May we had selected Colon Detective and began experimental lab work.