Team:Northern BC/Implementation

Proposed Implementation

Proposed Implementation:

Combining our hydroxylase gene, vitamin D-FRET binding proteins in E. coli will yield a construct that will display differing fluorescent signals in response to different levels of vitamin D. This would be contained in a chamber, where it would be used for analysis.

Our proposed end users are patients and clinicians in northern British Columbia hospitals. We anticipate that we can combine our method for detecting vitamin D deficiency with methods of extracting blood from vitamin D available in hospitals, allowing for cost-effective vitamin D detection. Once a blood sample is processed by other methods to extract vitamin D, our biosensor would be able to detect whether the vitamin D level would qualify as deficient or not. Thus, we envision that our project will be used by both clinicians and patients. Including a gene for chloramphenicol resistance in our plasmid also allows us to grow E. coli in our biosensor without having to worry about being out-competed by other species.

Currently, in northern British Columbia, Canada, vitamin D testing is rarely prescribed, as samples must be shipped almost 800 km south, where testing is done. This results in a costly and time-consuming process that is almost never utilized. Implementation of this product would provide the option to utilize this process locally, which will benefit the community as vitamin D deficiency is a prevalent issue in northern communities.

One of the safety factors to consider is the ethical considerations and requirements for obtaining blood samples to work with. However, by working in collaboration with healthcare facilities, we can circumvent this issue, as our project will not directly be involved in obtaining blood samples.