Team:Nanjing high school/Safety


1. Experiment Safety
    Our lab in Nanjing University of Chinese Medicine is classified as Biosafety Level 1, so the environment we work in is very safety. The lab strictly follows the safety instructions, and we also are under the guidance and supervision of our teachers: Dr. Lv and M.D. Shen. In order to ensure our own safety and prevent from contamination, we need to follow the lab safety protocols. For instance, we need to wear lab-gown, goggles and latex gloves all the time during experiment, also food and drinks are prohibited in the lab, as well as shorts and open-toe shoes.
2. Materials Safety
    Hazardous chemicals and bio-chemicals were used at a minimal level. If the chemicals needed are hazardous, it will require an area that is well ventilated and follows the standard strictly to operate. For instance, when screening for the E. coli that is successfully transformed, an antibiotic called Kanamycin is injected into the culture medium. In the experiment of gel electrophoresis, we need bromophenol blue to dye the proteins, and acrylamide to configure the gel, but both of the chemicals are toxic. While lysing the BV-2 cells to perform the Q-PCR assay, a toxic chemical Trizol is used. Although a certain amount of hazardous chemicals is used, every step that requires those chemicals are strictly supervised by our instructors. At the same time, protective measures are strictly carried out, and every chemical’s dosage is carefully controlled, monitored, and carefully disposed of after the experiment.
3. Disposal Safety
    In order to maintain the sanitation of the lab, the hazardous chemicals the experiments produced such as p-nitrophenol from the PPM1A enzyme activity screening is toxic, lab waste such as Petri dishes and culture medium will be disposed of into a specific trash bin, and extra liquid or solid chemicals such as disodium nitrophenyl phosphate will be disposed of into different specific trash receptacles, which will be carefully packaged and sent to a disposal facility afterwards to ensure no hazardous materials mismanaged.