Team:Nanjing high school/Notebook

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Notebook

14^th July

Ice breaking

Brainstorm: team name and merch

Design team logo

15^th July

Making LB solid culture medium

Add plasmids into competent cells

Cultivate E. coli bacteria

Prepare petri

16^th July

Making LB Liquid Culture Medium

Making PBS Buffer

Cultivating Monoclonal Bacterial Colonies

17^th July

Picking single bacterium to the liquid culture medium

Expand the bacteria cultivation

IPTG Induction of PPM1A Protein Expression

18^th July

Bacteria ultrasonic disruption

Bacteria Centrifugation

Protein Purification

19^th July

Cell recovery

PPM1A Protein Concentration

PPM1A purification

20^th July

Change Culture Medium

21^st July

Grind the tissue sample and place it on ice for cleavage

Centrifugation of RNA

Heat to dissolve with DEPC

22^nd July

RNA Inverse Transcription

PCR amplification

23^rd July

Produce a mix of forward primer, reverse primer and DEPC water

Produce a mix of cDNA, SYBR and primer mixture

PCR

Contact Info.

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