Team:NNU-China/Part/Parts
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K3868046 | Coding | SpCas9 | Yan Xu | 4107 |
| BBa_K3868047 | Regulatory | Pcas | Yan Xu | 226 |
| BBa_K3868048 | Coding | paraB-λ-red | Yan Xu | 2094 |
| BBa_K3868050 | Coding | sgRNA-8G | Yan Xu | 271 |
| BBa_K3868052 | Terminator | spy-ter | Yan Xu | 40 |
| BBa_K3868053 | Coding | dspCas9-linker | Yan Xu | 4467 |
| BBa_K3868054 | Coding | CDA | Yan Xu | 624 |
| BBa_K3868055 | Coding | UGI | Yan Xu | 252 |
| BBa_K3868056 | Coding | LVA | Yan Xu | 9 |
| BBa_K3868057 | Coding | sgRNA1 | Yan Xu | 271 |
| BBa_K3868058 | Coding | sgRNA2 | Yan Xu | 271 |
| BBa_K3868059 | Coding | sgRNA-CM | Yan Xu | 271 |
| BBa_K3868060 | Coding | MME | Yan Xu | 90 |
| BBa_K3868061 | Coding | Fa-AMP1 | Yan Xu | 120 |
| BBa_K3868062 | Coding | Alloferon-1 | Yan Xu | 39 |
| BBa_K3868063 | Coding | Oxysterlin 1 | Yan Xu | 117 |
| BBa_K3868064 | Coding | LL-37 | Yan Xu | 111 |
| BBa_K3868065 | Coding | SMAP-29 | Yan Xu | 87 |
| BBa_K3868066 | Coding | DCD-1L | Yan Xu | 141 |
| BBa_K3868067 | Coding | SPINK9 | Yan Xu | 189 |
| BBa_K3868068 | Coding | PEW300 | Yan Xu | 111 |
| BBa_K3868071 | Coding | EGFP-His-tag | Yan Xu | 735 |
| BBa_K3868072 | Coding | thrombin | Yan Xu | 18 |
| BBa_K3868074 | Regulatory | pYLXP-1 | Yan Xu | 1500 |
| BBa_K3868075 | Regulatory | pYLXP-2 | Yan Xu | 1500 |
| BBa_K3868076 | Regulatory | pYLXP-3 | Yan Xu | 1500 |
| BBa_K3868077 | Regulatory | pYLXP-4 | Yan Xu | 1500 |
| BBa_K3868078 | Regulatory | pYLXP-5 | Yan Xu | 1500 |
| BBa_K3868079 | Regulatory | pYLXP-6 | Yan Xu | 1500 |
| BBa_K3868080 | Regulatory | pYLXP-7 | Yan Xu | 1500 |
| BBa_K3868081 | Regulatory | pYLXP-8 | Yan Xu | 1482 |
| BBa_K3868082 | Regulatory | pYLXP-9 | Yan Xu | 1500 |
| BBa_K3868083 | Regulatory | pYLXP-10 | Yan Xu | 1500 |
| BBa_K3868084 | Regulatory | pYLXP-11 | Yan Xu | 1500 |
| BBa_K3868085 | Regulatory | pYLXP-12 | Yan Xu | 1500 |
| BBa_K3868086 | Regulatory | pYLXP-13 | Yan Xu | 1500 |
| BBa_K3868087 | Regulatory | pYLXP-14 | Yan Xu | 1500 |
| BBa_K3868088 | Regulatory | pYLXP-15 | Yan Xu | 1500 |
| BBa_K3868089 | Regulatory | pYLXP-16 | Yan Xu | 1500 |
| BBa_K3868090 | Regulatory | pYLXP-17 | Yan Xu | 1493 |
| BBa_K3868091 | Regulatory | pYLXP-18 | Yan Xu | 1500 |
| BBa_K3868092 | Regulatory | pYLXP-19 | Yan Xu | 1500 |
| BBa_K3868093 | Regulatory | pYLXP-20 | Yan Xu | 1500 |
| BBa_K3868094 | Coding | ID13 | Yan Xu | 120 |
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K3868095 | Composite | pCas | Yan Xu | 5269 |
| BBa_K3868096 | Composite | pTarget-8G | Yan Xu | 362 |
| BBa_K3868097 | Composite | pCBE | Yan Xu | 5604 |
| BBa_K3868098 | Composite | pTargetS | Yan Xu | 732 |
| BBa_K3868099 | Composite | pTarget-Donor | Yan Xu | 362 |
| BBa_K3868100 | Composite | pET-MME-eGFP | Yan Xu | 2022 |
| BBa_K3868101 | Composite | pET-Fa-AMP1-eGFP | Yan Xu | 2022 |
| BBa_K3868102 | Composite | pET-Alloferon-1-eGFP | Yan Xu | 2022 |
| BBa_K3868103 | Composite | pET-Oxysterlin 1-eGFP | Yan Xu | 2022 |
| BBa_K3868104 | Composite | pET-LL-37-eGFP | Yan Xu | 2022 |
| BBa_K3868105 | Composite | pET-SMAP-29-eGFP | Yan Xu | 2022 |
| BBa_K3868106 | Composite | pET-DCD-1L-eGFP | Yan Xu | 2022 |
| BBa_K3868107 | Composite | pET-SPINK9-eGFP | Yan Xu | 2004 |
| BBa_K3868108 | Composite | pET-PEW300-eGFP | Yan Xu | 2022 |
| BBa_K3868109 | Composite | pET-ID13-eGFP | Yan Xu | 2022 |
| BBa_K3868110 | Composite | pYLXP-1-Nluc | Yan Xu | 2022 |
| BBa_K3868111 | Composite | pYLXP-2-Nluc | Yan Xu | 2022 |
| BBa_K3868112 | Composite | pYLXP-3-Nluc | Yan Xu | 2022 |
| BBa_K3868113 | Composite | pYLXP-4-Nluc | Yan Xu | 2022 |
| BBa_K3868114 | Composite | pYLXP-5-Nluc | Yan Xu | 2022 |
| BBa_K3868115 | Composite | pYLXP-6-Nluc | Yan Xu | 2022 |
| BBa_K3868116 | Composite | pYLXP-7-Nluc | Yan Xu | 2015 |
| BBa_K3868117 | Composite | pYLXP-8-Nluc | Yan Xu | 2022 |
| BBa_K3868118 | Composite | pYLXP-9-Nluc | Yan Xu | 2022 |
| BBa_K3868119 | Composite | pYLXP-10-Nluc | Yan Xu | 2022 |
| BBa_K3868120 | Composite | pYLXP-11-Nluc | Yan Xu | 2022 |
| BBa_K3868121 | Composite | pYLXP-12-Nluc | Yan Xu | 2022 |
| BBa_K3868122 | Composite | pYLXP-13-Nluc | Yan Xu | 2022 |
| BBa_K3868123 | Composite | pYLXP-14-Nluc | Yan Xu | 2022 |
| BBa_K3868124 | Composite | pYLXP-15-Nluc | Yan Xu | 2022 |
| BBa_K3868125 | Composite | pYLXP-16-Nluc | Yan Xu | 2022 |
| BBa_K3868126 | Composite | pYLXP-17-Nluc | Yan Xu | 2015 |
| BBa_K3868127 | Composite | pYLXP-18-Nluc | Yan Xu | 2022 |
| BBa_K3868128 | Composite | pYLXP-19-Nluc | Yan Xu | 2022 |
| BBa_K3868129 | Composite | pYLXP-20-Nluc | Yan Xu | 2022 |
Among the 79 basic parts designed this year by ourselves, BBa_K3868100 - BBa_K3868109 are our favorite parts, which are based on the plasmid of pET-24a（Fig. 1）. The C-terminal of AMP was fused with eGFP in order to characterize the yield of AMP. The pET-24a was provided by our PI. Moreover, in order to purify the AMP, the enzyme loci of thrombin was inserted between AMP and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-AMP-eGFP. As a result, these pET-AMP-eGFP plasmids could not only indicate the expression level of AMPs by the fluorescence intensity of eGFP, but also be good for later purification and separation with His-Tag and the enzyme loci of thrombin.
