Team:NIT Warangal/Parts
Parts
Basic Parts
BBa_K4077000 was added as a basic part and is a laccase found in a fungus known as Melanocarpus albomyces. This sequence was optimised by GenScript for expression in Pichia pastoris plasmid pPIC9k. Second basic part BBa_K4077003 , the plasmid that we used for our experiments. It is a modified Pichia pastoris expression vector (pPIC9K).
For efficient protein folding in the endoplasmic reticulum, BBa_K4077005 was genetically engineered into the vector BBa_K4077003 . This is known as an alpha-factor secretion signal that is isolated from Saccharomyces cerevisiae. It was added to the registry in the basic part category. For the detailed information about these parts, click on the links given below.
| Name | Type | Description | Designer | Length (bp) |
|---|---|---|---|---|
| BBa_K4077000 | Protein Domain | Laccase from Melanocarpus albomyces (Q70KY3) |
Kavya Prince, Himanshu Ranjan | 1677 |
| BBa_K4077003 | Plasmid | Modified Pichia pastoris Plasmid pPIC9K |
Kavya Prince, Himanshu Ranjan | 10947 |
| BBa_K4077005 | Signalling | Native Saccharomyces cerevisiae alpha-factor secretion signal |
Himanshu Ranjan, Kavya Prince | 267 |
| BBa_K4077010 | Tag | Selectable marker to isolate Pichia recombinant strains. | Himanshu Ranjan | 2535 |
Composite Parts
Finally, the composite part we added to the registry was, BBa_K4077008 , which is the laccase gene insert, including promoter and terminators. It included the subparts, BBa_K3196036 and BBa_K3196026 , from iGEM 2019 team HUST China which were the AOX1 promoter and terminator sequences respectively. The sequences were found using the Registry BLAST. The AOX1 promoter sequence is isolated from the Pichia pastoris genome and genetically engineered into the pPIC9K expression vector.
The detailed information for this composite part is available on the main page of the registry page. Check it by clicking on the link available.
| Name | Type | Description | Designer | Length (bp) |
|---|---|---|---|---|
| BBa_K4077008 | DNA | Laccase from Melanocarpus albomyces gene insert | Kavya Prince, Himanshu Ranjan | 5662 |