Team:MiamiU OH/Parts

iGEM 2021 | Miami University

Title

Our team registered 40 parts within the iGEM registry, many of which are optimized for the photosynthetic chassis PCC 7942, a generally underrepresented but excellent chassis for future iGEM projects. Many of these parts have strong experimental evidence for functional operation. Overall, these parts made up the 4 plasmids our team used to transform cyanobacteria. We strongly recommend checking out our favorite parts list, as these parts were the best documented and have BioBrick compatibility!

Favorite Parts

Name Type Description Designer Length
    W BBa_K4047016 Coding Gentamicin resistance (GmR) for Synechococcus An Nguyen 534
    W BBa_K4047019 Coding Kanamycin Resistance for Synechococcus An Nguyen 816
    W BBa_K4047020 Regulatory Kanamycin Promoter for Synechococcus An Nguyen 106
    W BBa_K4047021 Terminator Kanamycin Resistance Terminator for Synechococcus An Nguyen 311
    W BBa_K4047031 Regulatory lac repressor An Nguyen 1083
    W BBa_K4047034 Coding transaldolase An Nguyen 1194

Parts

Name Type Description Designer Length
    W BBa_K4047016 Coding Gentamicin resistance (GmR) for Synechococcus An Nguyen 534
    W BBa_K4047019 Coding Kanamycin Resistance for Synechococcus An Nguyen 816
    W BBa_K4047020 Regulatory Kanamycin Promoter for Synechococcus An Nguyen 106
    W BBa_K4047021 Terminator Kanamycin Resistance Terminator for Synechococcus An Nguyen 311
    W BBa_K4047031 Regulatory lac repressor An Nguyen 1083
    W BBa_K4047034 Coding transaldolase An Nguyen 1194
U    BBa_K4047000 Primer primerT/TF_tal- forward Hope Kirby, Avery Imes 47
U    BBa_K4047001 Primer primerT_tal-reverse An Nguyen, Avery Imes 43
U    BBa_K4047002 Primer primerF_fbp-foward An Nguyen, Avery Imes 45
U    BBa_K4047003 Primer primerF/TF_fbp-reverse An Nguyen, Avery Imes 43
U    BBa_K4047004 Primer primerTF_tal-reverse An Nguyen, Avery Imes 38
U    BBa_K4047005 Primer primerTF_fbp-foward An Nguyen, Avery Imes 40
U    BBa_K4047007 Primer dSBPase_Backbone-fwd An Nguyen 37
U    BBa_K4047008 Primer dSBPase_Backbone-rev An Nguyen 18
U    BBa_K4047009 Primer dSBPase_glpX_US-fwd An Nguyen 41
U    BBa_K4047010 Primer dSBPase_glpX_US-rev An Nguyen 28
U    BBa_K4047011 Primer dSBPase_KanR-fwd An Nguyen 28
U    BBa_K4047012 Primer dSBPase_KanR-rev An Nguyen 34
U    BBa_K4047013 Primer dSBPase_glpX_DS-fwd An Nguyen 30
U    BBa_K4047014 Primer dSBPase_glpX_DS-rev An Nguyen 40
  W BBa_K4047015 Regulatory dSBPase-Backbonecomponent-GmRPromoter An Nguyen 316
  W BBa_K4047017 Terminator dSBPase-Backbone-GmrTerm An Nguyen 36
  W BBa_K4047018 Plasmid_Backbone dSBPase-Backbone An Nguyen 1781
    BBa_K4047022 DNA glpX_US An Nguyen 801
    BBa_K4047023 DNA glpX_DS An Nguyen 801
  W BBa_K4047024 Plasmid_Backbone pAM2991 An Nguyen 7973
    BBa_K4047025 Terminator rrnB T1 terminator An Nguyen 87
    BBa_K4047026 Terminator rrnB T2 terminoator An Nguyen 28
  W BBa_K4047027 Reporter SmR An Nguyen 792
     BBa_K4047028 Plasmid_Backbone bom An Nguyen 141
     BBa_K4047029 Plasmid_Backbone ori An Nguyen 589
    BBa_K4047030 Regulatory lacIq promoter An Nguyen 78
  W BBa_K4047032 Regulatory Trc promoter An Nguyen 30
     BBa_K4047033 Regulatory Lac operator An Nguyen 17
  W BBa_K4047035 Coding Fructose-1,6-bisphosphatase An Nguyen 1035
  W BBa_K4047036 Plasmid pGEM-tal An Nguyen 9172
  W BBa_K4047037 Plasmid pGEM-fbp An Nguyen 9013
  W BBa_K4047038 Plasmid pGEM-tal+fbp An Nguyen 10227
  W BBa_K4047039 Plasmid pGEM-SBPase (plasmid for deletion of SBPase) An Nguyen 4616

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