Team:IISER Kolkata

VECTOR ART
Problem Statement
TACKLING SUBCLINICAL BOVINE MASTITIS USING SYNBIO
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Problem Statement
TACKLING SUBCLINICAL BOVINE MASTITIS USING SYNBIO
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Problem Statement
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Milk and its derivatives are one of the primary sources of nutrition. Globally,more than 6 billion people consume milk and its products.
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480,256,000 tonnes of milk has been produced globally [ref] and 198,400.000 tonnes of milk has been produced in India, in 2020.
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Milk is the leading agricultural produce in India with the Dairy industry contributing 5.3% to India’s agricultural GDP.
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Due to a silent killer disease called Bovine Mastitis disease, Out of the loss incurred in milk production, 70% of it accounts to sub-clinical mastitis in India. As per NDRI, the estimated economic loss due to mastitis is INR 7165.51 crore per annum.
Problem Statement
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Absence of visible symptoms in the sub-clinical stage of the disease makes it difficult to detect the disease using available detection techniques.
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The disease in chronic stage can lead to fatality of bovines.
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Available treatment methods use broad-spectrum antibiotics.
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2730 tonnes of antibiotics are fed into the dairy sector.
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This leads to a steady development of antimicrobial resistance in mastitis-causing pathogenic bacteria.
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So Need of the Hour is to Develop an accurate, easy-to-use, point of care, portable, cheap detection kit Along with An Antibiotic-free treatment system.
OUR SOLUTION
We propose a 3-way breakthrough to tackle subclinical bovine mastitis
Prevention . Detection . Treatment
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Our Solution
Prevention is better than cure
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Maintaining proper hygienic methods while milking can stop the spread of this disease. Our Human Practices team has in-person spread awareness on the methods of hygienic milking among the stakeholders
our solution
Detection
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The present detection methods are expensive, not easily available, and are not precise. We propose a micro RNA-based colorimetric detection kit that will specifically detect the miRNA biomarkers in the milk sample using SHERLOCKv2 and CRISPR-responsive hydrogels in the microfluidic paper-based kits (μpad) to give visual colour for positive results. This will ensure the farmers have cheap, fast, point-of-care, portable, easy-to-use kits to detect the Bovine Mastitis disease in the subclinical stage and prevent the disease from progressing to chronic stages.
our solution
Treatment
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Broad-spectrum antibiotic-based existing treatment methods have led to antimicrobial resistance in mastitis causative pathogens. These treatment methods also lead to contamination of milk with such broad-spectrum antibiotics. This high concentration of antibiotics in milk is then consumed by humans on a daily basis which ultimately leads to antimicrobial resistance (AMR) in the causative pathogens of human diseases. We propose an antibiotics-free cure for sub-clinical bovine mastitis using narrow-spectrum bacteriocins. Our designed genetically modified organism (GMO) will at first sense the presence of sub-clinical mastitis-causing pathogens when injected via intra-mammary injection in the udder and will then release the bacteriocin Nisin PV along with the endonuclease DNase I leading to bacterial death and disintegration of the bacterial biofilms respectivel