Hello,this is a dynamic picture we design to present our collaboration relationship to you.
In the initial stage of the project design, we had many discussions with BUCT. In a discussion, we learned that BUCT wants to design a suicide system to ensure the safety of engineered bacteria. We suggested that they can use MazF as a toxin for the suicide of engineered bacteria. Meanwhile, we thought that the oxygen-sensitive promoter as a switch is a very delicate design.(More information you can click here)
Figure 2.The message and plasmid we sent to the BUCT.
Because we use small peptides such as LL-37 as anti-inflammatory peptides, but due to its size, we cannot use Tricine-Tris SDS-PAGE to characterize small peptides such as LL-37 on the electrophoresis. Just as we were in trouble, we learned that Jilin_China had used LL-37 in iGEM 2019. When we got in touch with the Jilin_China iGEM team, they were very willing to communicate with us. According to their experiment note in 2019, they gave us some useful advice. With the help of Jilin_China, we successfully characterized small peptides such as LL-37 on the electrophoresis with Tricine-Tris SDS-PAGE. In short, we were very grateful for the guidance given by Jilin_China.(More information you can click here)
Figure 3. Jilin_China.
In this year, we built partnership relationship with SZU-China. In the whole season, we energized everyone to grow with and to learn from one another. More information you can find here. (More information you can click here)
Figure 4. SZU-China
In the process of communicating model with NJTech_China, we learned that their machine learning models are clever, the difficult they faced was that their model’s accuracy cannot be improved. We suggested that they should try to use integrated learning such as gradient boosting decision tree (GBDT) or try to build long short-term memory (LSTM) models which is commonly used in text. However, the MCMC algorithm we used was very time-consuming, they suggested that we should use GPU calculations or try parallel calculations. We accepted their suggestion.(More information you can click here)
HUST2-China and we are both chose E.coli Nissle 1917 as chassis. In June 2021, we invited the HUST-iGEM team to jointly organize a Bioethics Debate Competition. The debate between humanists and scientists sparked a lot of enthusiasm. After the Bioethics Week, we maintained long-term contact. When we had some trouble in transforming plasmid into E.coli Nissle 1917, HUST2-China gave us E.coli Nissle 1917 strain to us. Later, as the project progressed, we had difficulty in molecular model. Due to the large size of NarX protein, the molecular model result was not accurate. HUST2-China suggested that we could choose the function domain to build model. We accepted their suggestion and we achieved the desired results in our model.(More information you can click here)
In march 2021, we organized a Bioethics Competition with HUST1-China participating in which sparked a lot of enthusiasm for bioethics among audiences. besides, during the project exchange session, they advised us to pay attention to feces disposal in the very beginning.(More information you can click here)
We provided the nitrate responsive promoter PyeaR for them. According to our practical experience. We shared our opinion with them about how to improve the wild-type promoter. In march, we were very glad to know that they accepted our invitation of debate competition.(More information you can click here)
We had a long-term cooperation relationship with XHD-Wuhan-B-China. During the competition, we provided experimental guidance and equipment for them. They were also one of participants of our debate in March. More information you can see here.(More information you can click here)
Because it was first time for YiYe- China to participate in iGEM. We had a virtual meeting with YiYe-China about model work. During the meeting, we gave them some suggestion in toehold modeling based on energy model.(More information you can click here)
iGEM Intestinal Congress
This year, we united BUCT-China, BNUZ-China, SZU-China, NJMU-China, NJU-China, and SUSTech_Shenzhen to accomplish a . We participated in collation of preventing horizontal gene transfer. To finish the Intestinal Program Reference Manual , we integrated the toxin- antitoxin system (TA system) in the iGEM registry of standard biological parts, and then found projects from previous iGEM competitions as examples to characterize biological function.( More information you can find here)
Figure 10.iGEM Intestinal Congress
We have attended the virtual Conference of China iGEMer Community, which gathered tons of iGEM teams from all parts of China. The participants included students, professors and some experts from various disciplines. During the conference, we presented our project for them. We also received some feedback from them.
Figure 11.Conference of China iGEMer