Team:CCU Taiwan/Protocol

Team:CCU Taiwan/Implementation

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Cloning

1. Primer PCR

2. Plasmid extraction

3. Ligation and transformation

4. Colony PCR

5. IPTG indution

6. SDS-PAGE and Coomassie blue staining

7. Purification

8. Others

Cell culture

Minimal inhibitory concentration(MIC)

Hemolysis

Cytotoxicity

Cell penetration ability test

Swelling test

Collagen layer

Absorptive layer

Thrombin cleavage assay