Team:BJU China/Protocol
Protocol
Protocol 1 - Molecular Cloning
Includes the following components:
1. E.coli transformation experiment
2. PCR experiment
3. DNA Restriction Enzyme Experiment
4. DNA Ligase Experiment
5. Colony PCR Experiment
6. SDS-PAGE Experiment
7. Preparation Competent cell
8. Preparation of Competent by One-Step Method
Protocol 2: Dye-sensitized Solar Kit
Dye-sensitized solar kit protocol
Protocol 3: Enzyme Activity
Includes the following components:
1. Test MaFMO enzyme activity
2. Test SttH enzyme activity
Protocol 4: Fermentation of 6BrIG
Fermentation of 6BrIG
Protocol 5: Mutation of TnaA gene
Mutation of TnaA gene
Protocol 6: 6Br-Indole to 6-BrIG production and extraction
Includes the following components:
1. Induction of MaFMO protein
2. 6BrIG Extraction
