As COVID-19 continues to ravage the world this year, most of our cooperation and communication with other teams are in the form of online meetings. What is particularly valuable is that during the winter vacation, some of the team members went back to their hometowns and had in-depth offline communication with iGEM team in their hometown colleges and universities, which also brought us valuable suggestions.
    One of them is the cooperation and exchange with Wuhan University YiYe-China team.

I. Pathological study of colorectal cancer

    YiYe-China's project is also a study on colorectal cancer, hoping to detect cancerous polyps through DNA methylation to minimize the discomfort of patients and some inconvenience of detection. This coincides with our thinking. Therefore, we discussed the current methods of colorectal cancer detection and treatment in depth, and compared and discussed the advantages and disadvantages of them.
   Before this, YiYe-China intended to detect the possibility of diagnosis of colorectal cancer by detecting the amount of relevant DNA expression in the sample target. Through investigation, tissue factor pathway inhibitor 2 (TFPI2) was found to be an essential DNA for CRC detection. The tfPI-2 of CRC polyp patients was significantly higher than that of healthy people. Since cancerous polyps can be excreted from the surface of our intestines in faeces, they aimed to investigate the role and efficiency of tfPI-2 methylation. The methylated DNA can then be compared with a standard DNA sample to see if it is positive or negative by DNA bisulfite transformation and PCR amplification. In addition, they aim to make methylated DNA glow by combining a red fluorescent protein, mCherry, near the end of a toe-cutting switch, so the detector can easily identify potential cancer cells with the naked eye.
    Comparatively speaking, we chose rRNA markers for early pre-diagnosis, and the discussion with YiYe-China brought us a lot of inspiration and suggestions on the selection of rRNA markers.

II. Functional cooperation on wechat applets

    In view of the convenience and real-time detection characteristics of wechat mini program, the BIT team specially designed a wechat mini program with sliding SlipChip for fluorescence detection of patient samples. Through communication with YiYe-China, we also decided to establish a platform that YiYe-China can use on wechat mini program.
    Let's take the operation interface of BIT team on wechat mini program as an example to briefly introduce its functions.

1. Team introduction interface

    This module is designed to display the main information of IGEM_BIT and Wuhan YiYe-China teams. The team logo and name of the two teams are displayed side by side at the top of the page. At the same time, a selection button will be set under the team logo. Users can click the button to browse more information about the corresponding team, including team introduction and team background, etc. The text and picture layout will be displayed in the form of a normal public account. The user can switch the lower content by switching the button below the team logo. (As shown in the figure)

Figure 1.Team Introduction Page

2. Bluetooth control function

Figure 2. Flow chart of work under Bluetooth control (module)

    In this page (as shown in Figure 2), the user can mainly realize the following three functions, and the commands are sent by Bluetooth.
    (1) Motor movement, multi-channel switching: there are four buttons on the page, corresponding to the front, back and left, each button has two modes, short press fine tuning, long press continuous movement. At the same time, there is also a button to finish the operation, to send the motor to end the movement, start to collect signals. The motor collects and processes the signal and automatically returns to the mobile phone.
    (2) Sliding SlipChip sliding, micro telescopic rod movement: there is a button on the page, press the button small program will send a command, the SlipChip will start sliding.
   (3) Temperature control: there is a button on the operation interface. Press the button and the small program will instruct the temperature control module to start working.

3. Accept data and test results

    This page displays the collected data and detection results received through Bluetooth. When the sliding SlipChip has completed all the working steps, the collected results will be returned to the small program through Bluetooth, and the specific results will be displayed on this page.

Figure 3. Interface diagram of data collection

   Figure 4 shows one of the code for the applet implementation interface.。

Figure 4

III. HP exchange and cooperation

    Before this, we also collected the adjustment questionnaire to timely understand the awareness of colorectal cancer in different populations. When we asked about the basic background of colorectal cancer, nearly half did not know or were completely unaware of the symptoms of colorectal cancer.

Figure 5

   Therefore, it is very important to popularize the information related to colorectal cancer.

Figure 6

    In the following what factors will increase the risk of colorectal cancer problem, think often intestinal disease and alcohol use increase the risk of the number is more, and think age and fewer diabetes increases the risk, and some subtle factors present uncertainty in investigation of many people is the attitude, Therefore, some obscure pathogenic factors are especially needed to be popularized to the public. These results indicate that many people do not know much about colorectal cancer and further illustrate the importance of the BIT and YiYe-China programs.

    Based on the above results, YiYe-China and we jointly carried out several online colorectal cancer publicity activities, which attracted the attention of the two universities and people from all walks of life. To improve people's understanding and prevention awareness of colorectal cancer through online publicity.

Figure 7

    The conference will introduce the clinical symptoms, dangers and mainstream detection methods of colorectal cancer, and summarize the problems existing in the existing detection technology and platform, such as high cost, long cycle, low tolerance of patients, which bring great inconvenience to the detection. Therefore, both teams hope to build sensitive and fast colorectal cancer detection systems.
    We also use other online promotions. On the Bilibili platform, the BIT team and the YiYe-China team broadcast the science popularization of colorectal cancer and the publicity of synthetic biology. The live broadcast introduced the clinical symptoms, hazards, mainstream, detection methods and the optimized detection methods respectively conceived by the two teams of colorectal cancer. The live broadcast was watched by nearly 5000 people (about 500 people). The Bilibili platform is mainly used

Figure 8. The sermon module

    by young people aged 14-30. At the same time, the low age and early onset of colorectal cancer is not well known to the public. Therefore, our live broadcast is for the "target audience". Through this live broadcast, we can let them know more about colorectal cancer. So we can introduce them to our programs and broaden the audience for our products. In this live broadcast, there were also CRC researchers who provided valuable advice during the live broadcast. His suggestions on "advantages and disadvantages of current markers and detection methods used" and "how to solve false positives" made us think deeply. Therefore, we also arranged special experiments in the follow-up improvement of the project to solve these problems. The problem. At the same time, he also suggested us to increase carrier experiments. After in-depth communication with him, we will also take this as one of our future improvement directions.