Team:BIT/Experiment

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Experiment

Experimental manual for feasibility verification of miRNA detection

1.Linkage reaction：10μL

2.LAMP reaction：20μL

3.CRISPR/Cas12a reaction：20μL

Feasibility verification of miRNA detection

1.Linkage reaction：10μL

2.LAMP reaction： 20μL

3.CRISPR/Cas12a reaction： 20μL

Experimental manual of freeze-dried balls replacing LAMP system

1.Linkage reaction
2.LAMP reaction：20μL

3.CRISPR/Cas12a reaction： 20μL

Experimental manual of fetal bovine serum substitution system

1.Linkage reaction：10μL

2.LAMP reaction：
3.CRISPR/Cas12a reaction：

Experimental manual for LAMP-free amplification experiment

1.Linkage reaction：20μL

2.CRISPR/Cas12a reaction