Team:IISER-Tirupati India/Attributions

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INDEX

• Faculty

• Alumni

• Misc.

• PHDs

• Help from external institute

• Team Work

The IISER Tirupati iGEM 2021 team would like to acknowledge the contributions of all those who helped us in our project with help and support in whichever way possible. Without their help, we would not have been able to take a step towards the actualisation of our project. 

Faculties

The faculties at our institute have been guiding us throughout our journey with their expertise in different fields. Thank you,

• Dr Vijayalakshmi V Subramanian, for your expert advice on cloning and protein expression in yeast, along with your constant support with project development and experimental protocols. We are thankful to receive the plasmids, and Saccharomyces cerevisiae strains from your lab. 
• Dr Nibedita Pal and Dr Hussain Bhukya, for your valuable advice on protein modelling and your encouragement for going beyond literature and exploring new possibilities.
• Dr Tapan Chandra Adhyapak and Dr Dileep Mampallil for your valuable guidance in modelling of the delivery of GMC, the first module of our project. 
• Dr Annapurna Devi Allu, for your constant support and guidance during our brainstorming sessions, encouraging us to take up challenges and think out of the pot (for our ideas based on plants). 
• Dr Ramkumar Sambasivan, for your invaluable advice on progesterone sensing during our brainstorming sessions which gave us a new path to explore for regulation of our design.
• Dr Swarup Roy Choudhury, for your advice on experimental protocols of yeast and for providing us with the required resources to conduct our experiments.
• Dr Ashwani Sharma, for your advice on vesicles and fluorescent proteins and providing us with the required resources to conduct our experiments.
• Dr Sreenivas Chavali, for your advice on protein structure and modelling during our brainstorming sessions.
• Dr Suchi Goel, for your invaluable advice during our brainstorming sessions and for providing resources for our experiments.
• Dr Sanjay Kumar, for your advice during our brainstorming sessions and providing us resources to develop our idea on cancer-nanobots during brainstorming sessions.
• Prof. K Vijayamohanan Pillai, for your advice during our brainstorming sessions for guiding us to develop our idea (for our idea to develop cancer-nanobots)

Alumni

Our team was in constant communication with some of the iGEM Alumni and some amazing science enthusiasts of IISER Tirupati, whose guidance and motivation helped us immensely throughout the iGEM journey. Thank you,

Bhabesh Kumar Tripathy, for your round-the-clock help and extensive contribution in shaping the project in every aspect. You are the mentor, aka advisor, that we officially couldn’t acknowledge, but we are grateful for your right pieces of advice. We are thankful for your infinite patience and to provide us with the possibility to ask and discuss questions at any time of the day. Your constant encouragement helped us stay motivated throughout our journey. 

Amogh Desai, for your persistent help and guidance with mathematical and protein modelling. We are thankful to you for providing us with invaluable resources and endless patience while we were learning different synthetic biology tools. We also appreciate your support and active participation in conducting the workshop with Agastya Foundation.

Ved Mahajan, for your unwavering help and guidance with fixing bugs in mathematical modelling. 

Dibya Saha for your constant guidance and help throughout the project. We thank you profusely for helping us understand various genders and the problems with current contraceptives with respect to the trans and intersex spectrum of individuals. Your persistent encouragement and motivation at every step boosted our confidence. We shall definitely make an iGEM memes and stickers collection for you. 

Shubhrika Jain, for your continuous support and guidance throughout the crucial period of our project. Your motivating words helped us find a way out of odd situations and your first-hand experience with virtual iGEM was a blessing.

Arya George, for your constant support and guidance throughout our iGEM journey for understanding several concepts of cloning and protein production in yeast and experimental protocols. Thank you for 

R Raajalaxmi, for your constant help and guidance with mathematical modelling.

Amartya Pal, for your help and support in experimental design for protein purification and Western blot.

Sanskar Agarwal, for your support during the development of our project and making our survey more inclusive, and for making us aware of more stakeholders. 

Deevitha, for your assistance in the approach towards our mathematical modelling problem. 

Mrugank Dake, for your support and guidance in the iGEM journey. 

MISC.

We were connected with several people who supported us for holistic development and representation of our project. Thank you, 

Agrima Sawhney and Mohit Kumar Pradhan, for your kind help in actualising our vision of Lab Eyes to make our labs more accessible.

Ms Tanya Agrawal, for your help and support in the Indian Sign Language Translation of words.

Ms Apurupa Vatsalya for providing us contacts for connecting us with several stakeholders and experts who helped us make our project more inclusive. 

Mrs Kadambari Lohiya, for facilitating us with an opportunity through the programme "STEM power for DoE girls" and our gratitude to Dr BP Pandey, Dr Shailendra Sharma for permitting us to conduct an informative workshop, Gene Gala, and all other teachers present throughout the sessions we conducted in collaboration with the iGEM 2021 Team of IISER Kolkata. 

We are grateful to the members of IISER Tirupati’s Prakriti Club who helped us organise the event on World Environment Day.

We would also like to thank Anita Banerjee, R Raajalaxmi, Lakshmi Veda Samhitha Peesapati, Vandana Rajesh Umbarkar, Sanskar Agrawal, Rakhee Rane, Pournima Bhangale, Akanksha Khurd, Mohan Angothu, Venkata Subbarao, Kruthika Avadhani, Mini K, Haseena P H, Kalpita Tripathy, Bhabesh Kumar Tripathy and Snehalata Pati for helping us prepare a unique stop-motion promo video for our project.

Malavika Rajesh, Gayathri Satheesh, Aparna C, Annal Baisil, Athmika Prajesh, Megha M and Sreelakshmi T N for helping us with our social media outreach activities during Sexual Health Week.

Adrita Das, Bajaj Mayur Manoj, Suyash Sawant, Rishabh Sharma, A Murali Krishna, Geetanjali, Neelima K, Namita Chutani, Anita Banerjee, Mr Anup Chandra Pal, Mr Mohan Mukesh Malviya, Mr Tharachand for helping us bring about our thoughts into action in-camera with your valuable contribution throughout the preparation of our Presentation Video.

PhDs

The pandemic placed a constraint on our access to the lab to carry out our experiments, however, the time that we spent in the lab was enriched with an enormous learning experience and cherishable memories because of the guidance and support of several iPhD and PhD students who helped us throughout. Thank you,

members of Prof BJ Rao’s Lab, Anupama Shirke, Bajaj Mayur Manoj, Gunjan and Komal Raina, for your immense guidance and support in conducting our experiments from our baby steps with preparing plates to purifying our desired proteins. We are grateful for your help during all our late-night experiments and your patience with our numerous questions.

members of Dr Sanjay Kumar’s Lab, Rahail Ashraf and Ravi(?) for guiding us with our experiments, handling instruments and providing us with required materials whenever required at the 11th hour!

members of Dr Vasudharani Devanathan’s Lab, Arulventhan M and Sapana Sharma for helping us with handling instruments and performing experiments. We are thankful for your support in providing us with resources for conducting our experiments.

members of Dr Suchi Goel’s Lab, Adrita Das, Bijayeeta Deb, Shukla Madhavi Sanjay and Neelima K for your persistent support and guidance throughout, we are also thankful to receive resources for our experiments from your lab. Our quick-query sessions were extremely important during the experiments.

members of Dr P Suresh Babu’s Lab, Namita Chutani and Himanshu for helping us with resources for conducting our experiments.

members of Dr Swarup Roy Choudhury’s Lab, Raju Kuiry, Jismon Jose and Dr. Bhagath Kumar Palaka for helping us with our experiments for cloning and affinity purification, we are grateful to receive your help with resources to conduct our experiments. 

members of Dr Raju Mukherjee’s Lab, Akanksha Agrawal, Lodhiya Tejan Harsukhbhai and Saniya Patil for helping us with our experiments, teaching us how to operate instruments and answering our queries multiple times throughout the journey.

Abin P George for helping us take the baby steps of acclimatizing in the lab and helping us with experimental techniques and operating instruments. 

A Murali Krishna from Dr Ashwani Sharma’s Lab, Pemmiraju Harish, Dona Maria Joseph from Dr Rajesh Vishwanathan, Sivakumar G from Dr Gopinath Purushothaman’s Lab, and Sonia M from Dr. Jatish Kumar’s Lab for helping us with resources throughout our experiments.

We thank all the PhDs and Ms Leelawati for helping us set up the autoclave multiple times throughout the day! We are thankful to all the guards and wardens who woke up in the middle of the night or early mornings to ensure that we reached our rooms/labs safely. 

Help from external institute

Piyush

Malhar

We are grateful to receive consistent help from Ayushi Hota, Dr Adyasha Mishra and Dr Ruchi Mishra from Maharaja Krishna Chandra Gajapati Medical College & Hospital, Odisha and Jihan Mohamad Iqbal from JIPMER, Pondicherry to understand the details of female reproductive system anatomy from the perspective of students and experts in medicine. 

Team Work

OviCloak wouldn’t have been possible without the contribution of each and every member of the team. We would like to elaborate on how we divided the work throughout the iGEM journey. 

Name	Role in Team	Attributions
Shreyas Samir Parkhie	Co-Team Leader, Wetlab, Human Practices and Outreach, Finances, Collaborations and Partnerships	Brainstorming for ideas, Literature survey, Wiki content writing and reviewing, Presentation video script and voice-over, Protocol compilation for Bacillus subtilis, Bacillus subtilis growth curve and transformation, Buffer preparation, Colony PCR, Content for deliverables, Partnership meetings, Collaborations, All India iGEM Meet representative, Sponsorship contacts, Responsibility of emails for all purposes, Podcast script and recording, iHP and organization of events, Social media management, Initial designing of brochure and posts
Komal Pati	Co-Team Leader, Wetlab, Protein Modelling, Design, Collaborations and Partnerships	Brainstorming for ideas, Literature survey for wet lab and protein modelling, Wiki content writing, Promo video script and voice-over, Presentation video script and voice-over, Protocol compilation for growth curves and Protein Purification, SDS-PAGE Gel preparation, Buffer preparation, Induction and Cell lysis, Protein Purification, Protein Modelling, Content for deliverables, Podcast recording, Collaborations, All India iGEM Meet representative, Responsibility of emails for all purposes, Graphic Design, iHP, Design of OviCloak Logo and Mascot Utilis
Akshay Babasaheb Tawale	Wetlab	Brainstorming for ideas, Literature survey, wiki content writing, Agarose Gel Electrophoresis, PCR Clean-up, Miniprep, Yeast Assembly Parts selection, Nanodrop, Gel extraction, Speedvac and ligation, PCR, Restriction digestion, Contact with NGO - Pankh Pune, Translation of content, Protocol compilation and Preliminary Check for Protocols
Ankush Kaushik	Wiki, Mathematical Modelling	Wiki coding and content writing, support in collaboration by helping other teams with their wiki, T-score calculator for T stretch, Growth modelling,
Ashwin A Pillai	Mathematical Modelling	Brainstorming for ideas, Literature survey, Wiki content writing, Coding for wiki, Growth Curve fitting, Growth 2 population graphs and derivation, Delivery system development and calculations,  Diffusion calculations - ficks diffusion, an alternative approach with ovum on the wall plus it’s rolling, Biosafety kill switch modelling
Ashwin Sharma	Wetlab	Brainstorming for ideas, Literature survey, Wiki content writing, Safety form and registry, Assembly Parts’ selection, Translation of content, E coli Competency and Transformation, Yeast Growth Curve, Translation of content, T-score calculator for T stretch, Podcast script and recording, Data mining for modelling,
Athira Anil	Wetlab	Brainstorming for ideas, Literature survey, Wiki content writing, compiling protocol for protein purification, Cell lysis and resuspension, Preparation of SDS-PAGE Gels, Buffer preparation, Patching for colony PCR, iHP, Content for Gene Gala, Podcast recording,
Fathima Aslaha TR	Wetlab, Human Practices and Outreach, Design, Collaborations	Brainstorming for ideas, Literature survey, Wiki content writing and layout, Protocol compilation for competency and transformation, Human Practices and Outreach, Designing brochure, social media posts, Reels and stories, Translation of content, Promo video voice-over, Presentation video, E coli growth curve, Competency and Transformation of E coli strains, Patching for colony PCR, Resuspension of DNA, Podcast recording, Social media management, Collaborations
Lochan R Chaudhari	Mathematical Modelling, Design, Collaborations	Brainstorming for ideas, Literature survey for Wetlab and Drylab, Wiki content writing, iHP, Promo video script, editing and voice over, Collaborations and Partnerships, Parts selection, Delivery, Growth 1 and 2 population graphs and derivation, Diffusion of ovastacin initial derivation, Ovastacin production model and graphs, Both kill switches: model and graphs, Responsibility of emails for mathematical modelling, Initial designing of brochure and posts, Collaborations
Md Adil Aman	Mathematical Modelling	Literature survey for mathematical modelling, Wiki content writing, Growth model  Growth curve fitting, Animation of mascot, Study of fluid dynamical model and derivation of continuous flow growth model
Niloufer Shanavas	Human Practices and Outreach, Finances, Collaborations	Brainstorming for ideas, Literature survey for wetlab, Initial Protocol for yeast transformation and bacterial culture, Wiki content writing, iHP and Outreach, Education and Communication activities, Collaboration activity for International surveys, Content for articles, Collaboration for drafting Handbook on Biotechnology Laws in India, Sponsorship contacts, Initial designing of brochure and posts
Prameya Pradyot Garge	Wetlab, Protein Modelling, Collaborations	Brainstorming for ideas, Literature survey for wet lab and protein modelling, Wiki content writing, Safety form and registry, Parts and Golden gate and Biobrick Assembly, Miniprep, Nanodrop, Restriction Digestion, Speedvac and ligation, Gel extraction, PCR, Resuspension of DNA, Translation of content, Protein modelling, Initial designing of brochure and posts, Collaborations,
Rajeshree Rajesh Umbarkar	Wetlab	Brainstorming for ideas, Literature survey, Wiki content writing, Promo video script and voice-over, Presentation video, Protocol compilation for SDS-PAGE, Staining, and Western Blot, Protein Purification, SDS-PAGE gel, Buffer preparation, Agarose gel preparation, Social media management, Initial designing of brochure and posts, Protocol compilation and Preliminary Check for Protocols
Shivam Kumar	Mathematical Modelling, Design	Brainstorming for ideas, Literature survey, Wiki content writing, Wiki Coding, Graphic Design, Handling social media, Sponsorship from InVideo, Promo Video, Podcast editing and recording, iHP, Cell encapsulation for delivery, Growth curve fitting, Ovastacin production graphs, Model in MATLAB, Comic Zine, Design of OviCloak Logo, Biosafety kill switch modelling
Shubhra Singhal	Wetlab, Human Practices and Outreach, Collaborations	Brainstorming for ideas, Literature survey, Content writing for project proposals, deliverables and wiki, Yeast Growth curve, Protocol compilation, SDS-PAGE Gel preparation, Buffer preparation, Protein purification, iHP, Outreach, Education and Communication Activities, Comic Zine content, LAB EYES, Database for trans and intersex individuals, Indian Sign Language Video, Podcast script, editing and recording, Social media management, Initial designing of brochure and posts, Collaborations

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