Difference between revisions of "Team:TecCEM/Parts"
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| − | <p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span class="wixGuard"></span></span></p></div><div id="comp-kuyqx34r" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuyqx34r" class="_1-6YJ _1Fe8-" data-image-info="{"containerId":"comp-kuyqx34r","displayMode":"fill","imageData":{"width":1600,"height":1200,"uri":"d211b2_64a4182e1e7f465f8965d8afcacf93a5~mv2.jpg","name":"T--TecCEM--lab4_edited.jpg","displayMode":"fill"}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_64a4182e1e7f465f8965d8afcacf93a5~mv2.jpg/v1/fill/w_520,h_390,al_c,q_80,usm_0.66_1.00_0.01/T--TecCEM--lab4_edited.webp"><img src="https://static.igem.org/mediawiki/2021/9/97/T--TecCEM--lab4.jpeg" alt="T--TecCEM--lab4_edited.jpg" style="width: 416px; height: 312px; object-fit: cover; object-position: 50% 50%;"></wix-image></div></div><section id="comp-kuyqyk4i" class="_3d64y"><div id="bgLayers_comp-kuyqyk4i" data-hook="bgLayers" class="_3wnIc | + | <p class="font_8" style="font-size: 17px; text-align: justify;"><span style="font-size:17px;"><span class="wixGuard"></span></span></p></div><div id="comp-kuyqx34r" class="XUUsC" title=""><div data-testid="linkElement" class="xQ_iF"><wix-image id="img_comp-kuyqx34r" class="_1-6YJ _1Fe8-" data-image-info="{"containerId":"comp-kuyqx34r","displayMode":"fill","imageData":{"width":1600,"height":1200,"uri":"d211b2_64a4182e1e7f465f8965d8afcacf93a5~mv2.jpg","name":"T--TecCEM--lab4_edited.jpg","displayMode":"fill"}}" data-bg-effect-name="" data-is-svg="false" data-is-svg-mask="false" data-image-zoomed="" data-has-ssr-src="" data-src="https://static.wixstatic.com/media/d211b2_64a4182e1e7f465f8965d8afcacf93a5~mv2.jpg/v1/fill/w_520,h_390,al_c,q_80,usm_0.66_1.00_0.01/T--TecCEM--lab4_edited.webp"><img src="https://static.igem.org/mediawiki/2021/9/97/T--TecCEM--lab4.jpeg" alt="T--TecCEM--lab4_edited.jpg" style="width: 416px; height: 312px; object-fit: cover; object-position: 50% 50%;"></wix-image></div></div><section id="comp-kuyqyk4i" class="_3d64y"><div id="bgLayers_comp-kuyqyk4i" data-hook="bgLayers" class="_3wnIc"> |
| − | <div id="bgLayers_comp-kuyqyk65" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuyqyk65" class="_2GUhU"></div></div><div data-mesh-id="comp-kuyqyk65inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuyqyk65inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuyqzgbp" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:31px; text-align:justify"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="font-size:31px"><span style="font-weight:bold">Biosensor Parts:</span></span></span></p> | + | <div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuyqyk4i" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuyqyk65" class="_1vNJf"> |
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| + | <div id="bgLayers_comp-kuyqyk65" data-hook="bgLayers" class="_3wnIc"> | ||
| + | <div data-testid="colorUnderlay" class="_3KzuS _3SQN-"></div><div id="bgMedia_comp-kuyqyk65" class="_2GUhU"></div></div><div data-mesh-id="comp-kuyqyk65inlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuyqyk65inlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuyqzgbp" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:31px; text-align:justify"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold"><span style="font-size:31px"><span style="font-weight:bold">Biosensor Parts:</span></span></span></p> | ||
<br><br> | <br><br> | ||
<p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px;"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">To complete the design of the Biosensor at a molecular level, we created two basic parts that code for two proteins and two composite parts. The basic parts correspond to the coding sequences for our biosensor while the composite parts were designed for the expression of the proteins.</span></span></p></div><div id="comp-kuypp37c" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:right"><span style="font-size:17px"><span class="color_15"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">To read more information about these parts, please check the information </span></span><span style="font-family:wfont_1229ac_98e6104439904642a97dab8221f26c85,wf_98e6104439904642a97dab822,orig_roboto_bold;"><span style="color:#001489;"><span style="text-decoration:underline">here</span></span></span><span class="color_15"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">. </span></span></span></h2></div> | <p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px;"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">To complete the design of the Biosensor at a molecular level, we created two basic parts that code for two proteins and two composite parts. The basic parts correspond to the coding sequences for our biosensor while the composite parts were designed for the expression of the proteins.</span></span></p></div><div id="comp-kuypp37c" class="_1Q9if" data-testid="richTextElement"><h2 class="font_2" style="font-size:17px; text-align:right"><span style="font-size:17px"><span class="color_15"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">To read more information about these parts, please check the information </span></span><span style="font-family:wfont_1229ac_98e6104439904642a97dab8221f26c85,wf_98e6104439904642a97dab822,orig_roboto_bold;"><span style="color:#001489;"><span style="text-decoration:underline">here</span></span></span><span class="color_15"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">. </span></span></span></h2></div> | ||
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</div></div></div></div></section> | </div></div></div></div></section> | ||
| − | <section id="comp-kuypp37w" class="_3d64y"><div id="bgLayers_comp-kuypp37w" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class=" | + | <section id="comp-kuypp37w" class="_3d64y"><div id="bgLayers_comp-kuypp37w" data-hook="bgLayers" class="_3wnIc"> |
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| + | <div data-testid="colorUnderlay" class="_3SQN-"></div><div id="bgMedia_comp-kuypp37w" class="_2GUhU"></div></div><div data-testid="columns" class="_1uldx"><div id="comp-kuypp38o" class="_1vNJf"><div id="bgLayers_comp-kuypp38o" data-hook="bgLayers" class="_3wnIc"><div data-testid="colorUnderlay" class="_3SQN-"></div><div id="bgMedia_comp-kuypp38o" class="_2GUhU"></div></div><div data-mesh-id="comp-kuypp38oinlineContent" data-testid="inline-content" class=""><div data-mesh-id="comp-kuypp38oinlineContent-gridContainer" data-testid="mesh-container-content"><div id="comp-kuyqaa24" class="_1Q9if" data-testid="richTextElement"><h1 class="font_0" style="font-size: 33px;"><span style="font-size:33px;"><span style="font-family:wfont_299caa_3396eff86734448f84bd1bfa8335f847,wf_3396eff86734448f84bd1bfa8,orig_nunito_bold;">Selection Marker Parts:</span></span></h1></div><div id="comp-kuypp394" class="_1Q9if" data-testid="richTextElement" style="text-align:justify;"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto"><span style="font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">In order to design the new selection marker we proposed, we had to produce several pieces of DNA. The purpose of these pieces was to create a new strain of <span style="font-style:italic">E. coli</span> with the following characteristics: Knockout of genes oxyR, gldA and glpK and its substitution with genes PuDHT, mRFP and aldO. Besides, we designed a new plasmid based on some elements from the pUC plasmid. However, this new construct is Biobrick Assembly Friendly and has our new Selection Cassette.</span></span></span></p></div><div id="comp-kuypp39b" class="_1Q9if" data-testid="richTextElement"><p class="font_8" style="font-size:17px; text-align:justify"><span style="font-size:17px"><span style="font-family:wfont_069f35_23de2075afcc42cc848bac4f05c000f8,wf_23de2075afcc42cc848bac4f0,orig_roboto">To read more information about these parts, please check the information<span style="font-weight:bold"> <span style="color:#001489;"><span style="text-decoration:underline">here. </span></span></span></span></span></p></div> | ||
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Revision as of 02:20, 20 October 2021
Home
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Overview
The parts we created
We designed several biobricks that helped us reach our objectives. All parts we developed were created using Snapgene while the synthesis of the DNA blocks were obtained from our sponsor IDT.
We created two main sets of parts: The Biosensor Parts and the Selection Marker Parts.
Biosensor Parts:
To complete the design of the Biosensor at a molecular level, we created two basic parts that code for two proteins and two composite parts. The basic parts correspond to the coding sequences for our biosensor while the composite parts were designed for the expression of the proteins.
To read more information about these parts, please check the information here.
| Part name | Type | Short Description | Length (bp) |
|---|---|---|---|
| BBa_k3809010 | Coding | ESR1 with Linker, His Tag and Signal Peptide | 1884 |
| BBa_k3809011 | Reporter | mRFP with Linker, His Tag and Signal Peptide | 774 |
| BBa_k3809012 | Composite | Modified ESR1 protein under strong constitutive promoter and RBS | 1948 |
| BBa_k3809013 | Composite | Modified mRFP protein under strong constitutive promoter and RBS | 838 |
Selection Marker Parts:
In order to design the new selection marker we proposed, we had to produce several pieces of DNA. The purpose of these pieces was to create a new strain of E. coli with the following characteristics: Knockout of genes oxyR, gldA and glpK and its substitution with genes PuDHT, mRFP and aldO. Besides, we designed a new plasmid based on some elements from the pUC plasmid. However, this new construct is Biobrick Assembly Friendly and has our new Selection Cassette.
To read more information about these parts, please check the information here.
| Part name | Type | Short Description | Length (bp) |
|---|---|---|---|
| BBa_K3809001 | Coding | Novel Selection Cassette katE. | 2562 |
| BBa_K3809004 | Coding | aldO | 1257 |
| BBa_K3809006 | Coding | PuDHT | 1728 |
| BBa_K3809014 | Reporter | mRFP + oxyR homology regions + FLP recognition sequence | 1040 |
| BBa_K3809003 | Composite | katE regulated by BBa_J23100 | 2462 |
| BBa_K3809005 | Composite | aldO + glpK homology regions | 1487 |
| BBa_K3809007 | Composite | PuDHT + gldA homology regions | 1958 |
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