Team:TecCEM/Parts

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Overview

The parts we created

We designed several biobricks that helped us reach our objectives. All parts we developed were created using Snapgene while the synthesis of the DNA blocks were obtained from our sponsor IDT.

We created two main sets of parts: The Biosensor Parts and the Selection Marker Parts.

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Biosensor Parts:

To complete the design of the Biosensor at a molecular level, we created two basic parts that code for two proteins and two composite parts. The basic parts correspond to the coding sequences for our biosensor while the composite parts were designed for the expression of the proteins.

To read more information about these parts, please check the information here. 

Part name	Type	Short Description	Length (bp)
BBa_K3809001	Coding	ESR1 with Linker, His Tag and Signal Peptide	1884
BBa_K3809011	Reporter	mRFP with Linker, His Tag and Signal Peptide	774
BBa_K3809012	Composite	Modified ESR1 protein under strong constitutive promoter and RBS	1948
BBa_K3809013	Composite	Modified mRFP protein under strong constitutive promoter and RBS	838

Selection Marker Parts:

In order to design the new selection marker we proposed, we had to produce several pieces of DNA. The purpose of these pieces was to create a new strain of E. coli with the following characteristics: Knockout of genes oxyR, gldA and glpK and its substitution with genes PuDHT, mRFP and aldO. Besides, we designed a new plasmid based on some elements from the pUC plasmid. However, this new construct is Biobrick Assembly Friendly and has our new Selection Cassette.

To read more information about these parts, please check the information here. 

Part name	Type	Short Description	Length (bp)
BBa_K3809001	Coding	Novel Selection Cassette katE.	2562
BBa_K3809004	Coding	aldO	1257
BBa_K3809006	Coding	PuDHT	1728
BBa_K3809014	Reporter	mRFP + oxyR homology regions + FLP recognition sequence	1040
BBa_K3809003	Composite	katE regulated by BBa_J23100	2462
BBa_K3809005	Composite	aldO + glpK homology regions	1487
BBa_K3809007	Composite	PuDHT + gldA homology regions	1958