Difference between revisions of "Team:HK GTC/Parts"

Line 23: Line 23:
 
</html>
 
</html>
 
       <div class="content-wrapper parts center">
 
       <div class="content-wrapper parts center">
−
<h1> <font color="black"> Parts </font> </h1>
+
        <h1> <font color="black"> Parts </font> </h1>
 
         <groupparts>iGEM21 HK_GTC</groupparts>
 
         <groupparts>iGEM21 HK_GTC</groupparts>
 
         <html>
 
         <html>
 
         </div>
 
         </div>
  
−
         <h2 class="intro">Overview</h2>
+
         <span class="material-icons to-top"><a href="#the-top">expand_less</a></span>
−
        <p class="intro">We created 4 basic parts and 2 composite parts.</p>
+
−
       
+
−
        <div class="eachpart">
+
−
        <h2> BBa_K3787001 </h2>
+
−
        <p> Wild Type PETase <br>
+
−
The coding sequence for wild type PETase, codon optimised for the gene expression in E. coli. It serves as a standard for comparison of performance of PETase mutants. <br></p>
+
−
        </div>
+
  
−
         <div class="eachpart">
+
  <div class="section-heading" id="the-top">
−
        <h2> BBa_K3787002 </h2>
+
      <div class="mask"></div>
−
        <p> PETase(WT)-MHETase chimera <br>
+
      <h1>Project Description</h1>
 +
  </div>
 +
 
 +
  <div class="section-wrapper">
 +
 
 +
      <h2>BBa_K3787001 </h2>
 +
      <p>
 +
         PETase(WT)-MHETase chimera<br>
 +
        Composite part (Coding)<br>
 +
        Length: 2715 bp<br>
 +
        The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E.
 +
        coli.<br>
 +
        It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid
 +
        serine glycine linker.<br>
 +
 
 +
      </p>
 +
  </div>
 +
 
 +
  <div class="eachpart">
 +
      <h2> BBa_K3787002 </h2>
 +
      <p> PETase(WT)-MHETase chimera <br>
 
         Composite part (Coding) <br>
 
         Composite part (Coding) <br>
 
         Length: 2715 bp <br>
 
         Length: 2715 bp <br>
−
         The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.<br>
+
         The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E.
−
         It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker. </p>
+
        coli.<br>
−
         </div>
+
         It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid
 +
        serine glycine linker. </p>
 +
  </div>
 +
 
 +
  <div class="eachpart">
 +
      <h2> BBa_K3787004 </h2>
 +
      <p>12 amino acid serine glycine linker<br>
 +
         Basic part (Coding)<br>
 +
        Length: 36 bp<br>
 +
        The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form
 +
        chimeric proteins.
 +
        <br>
 +
      </p>
 +
  </div>
 +
 
 +
  <div class="eachpart">
 +
      <h2> BBa_K3787008 </h2>
 +
      <p>
 +
        MHETase R914 Reverse primer<br>
 +
        Basic part (Primer)<br>
 +
        Length: 20 bp<br>
 +
        The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.<br>
  
−
        <div class="eachpart">
+
      </p>
−
        <h2> BBa_K3787003 </h2>
+
−
        <p> I.s. MHETase <br>
+
−
Basic part (Coding) <br>
+
−
Length: 1809 bp <br>
+
−
The coding sequence for MHETase, codon optimized for the gene expression in E. coli. <br>
+
−
The sequence comes from the bacteria Ideonella sakaiensis. It is used in enzyme cocktail experiments.
+
−
</p>
+
−
        </div>
+
  
−
<div class="eachpart">
+
  </div>
−
        <h2> BBa_K3787004 </h2>
+
−
        <p> 12 amino acid serine glycine linker <br>
+
−
Basic part (Coding)
+
−
Length: 36 bp <br>
+
−
The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.
+
−
</p>
+
−
        </div>
+
  
−
        <div class="eachpart">
+
  <div class="eachpart">
−
        <h2> BBa_K3787008 </h2>
+
      <h2> BBa_K3787009 </h2>
−
        <p> MHETase R914 Reverse primer <br>
+
      <p>
−
Basic part (Primer) <br>
+
        MHETase F416 Forward primer<br>
−
Length: 20 bp <br>
+
        Basic part (Primer)<br>
−
The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.  
+
        Length: 20 bp<br>
−
</p>
+
        The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.<br>
−
        </div>
+
      </p>
 +
  </div>
  
−
        <div class="eachpart">
+
  <div class="clear"></div>
−
         <h2> BBa_K3787009 </h2>
+
 
−
         <p> MHETase F416 Forward primer <br>
+
  <footer>
−
Basic part (Primer) <br>
+
      <div>
−
Length: 20 bp <br>
+
         <h1>GT COLLEGE iGEM2021</h1>
−
The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.
+
         <img width="30%" src="https://static.igem.org/mediawiki/2021/7/78/T--HK_GTC--logo.png" alt="">
−
</p>
+
      </div>
−
         </div>
+
      <div>
 +
        <h1>Follow Us!</h1>
 +
        <a href="https://www.instagram.com/gtigemteam/">
 +
            <i class="icon fab fa-instagram fa-2x"></i>
 +
         </a>
 +
      </div>
 +
      <div>
 +
        <h1>Contact:</h1>
 +
        <p>igemteam.gt@gmail.com</p>
 +
      </div>
 +
  </footer>
  
 
       </body>
 
       </body>
 
</html>
 
</html>

Revision as of 10:19, 17 October 2021

HK_GTC 2021 Homepage

Parts

Parts 

       <groupparts>iGEM21 HK_GTC</groupparts>
expand_less

Project Description

BBa_K3787001

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787002

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787004

12 amino acid serine glycine linker
Basic part (Coding)
Length: 36 bp
The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.

BBa_K3787008

MHETase R914 Reverse primer
Basic part (Primer)
Length: 20 bp
The MHETase R914 reverse primer, for amplification of MHETase and chimeric proteins by PCR.

BBa_K3787009

MHETase F416 Forward primer
Basic part (Primer)
Length: 20 bp
The MHETase F416 forward primer, for amplification of MHETase and chimeric proteins by PCR.

GT COLLEGE iGEM2021

Follow Us!

Contact:

igemteam.gt@gmail.com