Team:HK GTC/Notebook

HK_GTC 2021 Homepage

expand_less

Notebook

August 2020

06/08/2020

Protein extraction of WT PETase and mutant PETase, S245I from E. coli

26/08/2020

Determination of protein concentration using Bradford assay

September 2020

09/09/2020

SDS-PAGE for PETase-S245I and PETase(WT)

October 2020

24/10/2020

Plastic photography in Lei Yu Mun

November 2020

28/11/2020

Plastic photography in Cheung Chau

December 2020

28/12/2020

Bacterial transformation
Transformation of MHETase, WT PETase-MHETase, and S245I PETase-MHETase sequence within PET21b vector into E. coli C41(DE3) and TOP10 competent cells by heat shock of 42°C

January 2021

21/01/2021

PET film digestion at 30°C for 96 hours

25/01/21

Digested PET film processing

29/01/21

Examination of digested PET films under SEM

February 2021

03/02/21

HPLC of solution from digestion of PET film

10/02/21

Colony PCR
used MHETase primer
Colony PCR of constructs transformed into C41(DE3) competent cells

March 2021

19/3/2021

Conducted survey pilot testing in G.T. College

April 2021

26/04/21

Interview with Professor Jacky Ngo, CUHK

30/04/21

Interview with Professor T.F. Chan, CUHK

May 2021

05/05/21

Protein extraction of WT PETase, S245I PETase (1) and (2), MHETase, WT PETase-MHETase and S245I PETase-MHETase

24/05/21, 28/05/21

Conducted physical full-scale survey and plastic tagging activity in G.T. College

31/05/21

Interview with Mr. Sidhant Gupta and Mr. Utkarsh Goel, Clearbot

June 2021

24/06/21

SDS-PAGE of extracted protein constructs on 05/05/21

July 2021

13/07/21

Bradford Assay of WT PETase, S245I PETase (1) and MHETase

16/07/21

PET film digestion at 30°C for 96 hours

17/07/21

Protein extraction of WT PETase, S245I PETase and WT PETase-MHETase chimera

19/07/21

SDS PAGE of extracted proteins from 17/07/21

20/07/21

Digested PET film processing
PET film digestion using enzyme cocktails on 16/07/21

20/07/21

Virtual meetup with Cornell Team

20/7/21 – 24/7/21

Large scale protein extraction (400mL bacterial culture)

21/07/21

HPLC of solutions after PET film digestion using enzyme cocktails

21/07/21

Physical meetup with HKUST team

21/07/21

Plastic photography in Lantau Island

25/07/21

Virtual meetup with Stony Brook team

26/07/21

SDS PAGE of extracted proteins from 21 – 24/7/21

26/07/21

Physical meetup with HKU team

26/07/21

PET film digestion

30/07/21

PET film processing

August 2021

02/08/21

HPLC of solutions after PET film digestion using chimeric and single enzymes

04/08/21

Interview with Ms. Dana Winograd, Plastic Free Seas

09/08/21

Sharing session by Ms. Dana Winograd, Plastic Free Seas

11/08/21

iGEM Symposium 2021, HKUST

20/8/21

SEM
We examined the digested PET film using 16μL chimeric proteins under the SEM.

September 2021

13/09/21 – 16/09/21

SDS-PAGE and Western blot

15/09/21

iGEM sharing session for G7 and G8 GT school students

30/09/21

Interview with Mr. Nigel Mattraver, New Life Plastics

October 2021

15/10/21

Interview with Mr. Hamlet Yu, HKSTP, Mr. Sam Chow and Mr. Kelvin Pong from BioArchitect

16/10/21

Hong Kong High School iGEM Symposium 2021

GT COLLEGE iGEM2021

Follow Us!

Contact:

igemteam.gt@gmail.com