Protein extraction of WT PETase and mutant PETase, S245I from E. coli
Determination of protein concentration using Bradford assay
SDS-PAGE for PETase-S245I and PETase(WT)
Plastic photography in Lei Yu Mun
Plastic photography in Cheung Chau
Bacterial transformation Transformation of MHETase, WT PETase-MHETase, and S245I PETase-MHETase sequence within PET21b vector into E. coli C41(DE3) and TOP10 competent cells by heat shock of 42°C
PET film digestion at 30°C for 96 hours
Digested PET film processing
Examination of digested PET films under SEM
HPLC of solution from digestion of PET film
Colony PCR used MHETase primer Colony PCR of constructs transformed into C41(DE3) competent cells
Conducted survey pilot testing in G.T. College
Interview with Professor Jacky Ngo, CUHK
Interview with Professor T.F. Chan, CUHK
Protein extraction of WT PETase, S245I PETase (1) and (2), MHETase, WT PETase-MHETase and S245I PETase-MHETase
Conducted physical full-scale survey and plastic tagging activity in G.T. College
Interview with Mr. Sidhant Gupta and Mr. Utkarsh Goel, Clearbot
SDS-PAGE of extracted protein constructs on 05/05/21
Bradford Assay of WT PETase, S245I PETase (1) and MHETase
Protein extraction of WT PETase, S245I PETase and WT PETase-MHETase chimera
SDS PAGE of extracted proteins from 17/07/21
Digested PET film processing PET film digestion using enzyme cocktails on 16/07/21
Virtual meetup with Cornell Team
Large scale protein extraction (400mL bacterial culture)
HPLC of solutions after PET film digestion using enzyme cocktails
Physical meetup with HKUST team
Plastic photography in Lantau Island
Virtual meetup with Stony Brook team
SDS PAGE of extracted proteins from 21 – 24/7/21
Physical meetup with HKU team
PET film digestion
PET film processing
HPLC of solutions after PET film digestion using chimeric and single enzymes
Interview with Ms. Dana Winograd, Plastic Free Seas
Sharing session by Ms. Dana Winograd, Plastic Free Seas
iGEM Symposium 2021, HKUST
SEM We examined the digested PET film using 16μL chimeric proteins under the SEM.
SDS-PAGE and Western blot
iGEM sharing session for G7 and G8 GT school students
Interview with Mr. Nigel Mattraver, New Life Plastics
Interview with Mr. Hamlet Yu, HKSTP, Mr. Sam Chow and Mr. Kelvin Pong from BioArchitect
Hong Kong High School iGEM Symposium 2021
