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| + | We are designing a biosensor for the detection of Endocrine Disruptive Compounds (EDCs) in samples of bottled water. This biosensor will work through the immobilization of protein receptor hER alpha on a quartz, which is part of a piezoelectric sensor. The protein will capture these chemicals, while the piezoelectric sensor will sense the change of mass through a change of the natural resonance of the quartz. This signal will be received and interpreted by a circuit of our own design and will give us information about the concentration of EDCs on the sample of water. We are going to focus on the enzymatic degradation of these compounds as well, through the expression of a Laccase. We plan on introducing this step as part of the purification process of fresh water. Our main goal is to guarantee EDC-free water to the general population, reducing the risk of developing any serious illness. | ||
| + | In addition, we want to design a novel selection marker which includes the use of glycerol and a modified strain of E. coli which can’t metabolize this compound without a selection cassette. The proposed design will allow us to reduce the antibiotic selection markers used in laboratory protocols by instead using glycerol; diminishing the risk to human health by an accidental release of antibiotic resistant bacteria. | ||
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Revision as of 00:01, 17 October 2021
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Título 1
We are designing a biosensor for the detection of Endocrine Disruptive Compounds (EDCs) in samples of bottled water. This biosensor will work through the immobilization of protein receptor hER alpha on a quartz, which is part of a piezoelectric sensor. The protein will capture these chemicals, while the piezoelectric sensor will sense the change of mass through a change of the natural resonance of the quartz. This signal will be received and interpreted by a circuit of our own design and will give us information about the concentration of EDCs on the sample of water. We are going to focus on the enzymatic degradation of these compounds as well, through the expression of a Laccase. We plan on introducing this step as part of the purification process of fresh water. Our main goal is to guarantee EDC-free water to the general population, reducing the risk of developing any serious illness. In addition, we want to design a novel selection marker which includes the use of glycerol and a modified strain of E. coli which can’t metabolize this compound without a selection cassette. The proposed design will allow us to reduce the antibiotic selection markers used in laboratory protocols by instead using glycerol; diminishing the risk to human health by an accidental release of antibiotic resistant bacteria.
Título 2
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Título 3
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Título 4
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Fig 1. Lorem ipsum dolor sit amet, consectetur adipisicing elit. Itaque, nam, maiores. Inventore ex eum doloremque eligendi id, voluptates quod illum, dolore deserunt iste alias. Dolor libero illo hic, temporibus harum.
Título 5
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Et voluptatem iste aperiam perspiciatis modi officia necessitatibus, veritatis quasi aliquam quam!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Perferendis architecto voluptas unde expedita labore, ipsa, vel, minus autem modi nisi quo ab esse pariatur voluptatibus tempora! Enim nihil, eum officiis!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Eligendi repellat omnis magnam recusandae necessitatibus optio, nostrum perferendis assumenda, culpa. Tenetur amet at temporibus unde ratione quaerat eos quam iusto. Deserunt.Lorem ipsum dolor sit Lorem ipsum dolor sit amet, consectetur adipisicing elit. Neque dolores aperiam error, odit quas illo illum cum earum fuga id autem, inventore voluptatibus recusandae, voluptatum distinctio veritatis, ducimus repellat enim!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Pariatur cupiditate totam eos debitis necessitatibus natus ipsa, possimus reiciendis quaerat. Harum sit ducimus voluptatum, id quia fuga, sequi tempore perferendis veritatis.Lorem ipsum dolor sit amet, consectetur adipisicing elit. Adipisci expedita eum fugit culpa corporis laborum perferendis, magnam iure! Et voluptatem iste aperiam perspiciatis modi officia necessitatibus, veritatis quasi aliquam quam!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Perferendis architecto voluptas unde expedita labore, ipsa, vel, minus autem modi nisi quo ab esse pariatur voluptatibus tempora! Enim nihil, eum officiis!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Eligendi repellat omnis magnam recusandae necessitatibus optio, nostrum perferendis assumenda, culpa. Tenetur amet at temporibus unde ratione quaerat eos quam iusto. Deserunt.Lorem ipsum dolor sit Lorem ipsum dolor sit amet, consectetur adipisicing elit. 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Altan A et al. (2020)
Referencia
[3] Altan, A., & Karasu, S. (2020). Recognition of COVID-19 disease from X-ray images by hybrid model consisting of 2D curvelet transform, chaotic salp swarm algorithm and deep learning technique. Chaos, Solitons and Fractals, 140, 110071. https://doi.org/10.1016/j.chaos.2020.110071
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