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<h2 id="current">Safe organisms</h2> | <h2 id="current">Safe organisms</h2> | ||
− | <p>Our team selects non-pathogenic classis for usage. They are safe strains of Escherichia coli called DH5α and C41(DE3) to transform recombinant plasmids - pET21b (+). It carries the protein-coding sequences to produce the protein chimeras. Both of the E.coli strains that we choose have no harm on humans nor the natural environment. As PETase is toxic to E.coli, using the strain C41(DE3) allows the expression of toxic proteins, so that our protein chimeras can be expressed. <br> | + | <p>Our team selects non-pathogenic classis for usage. They are safe strains of <i>Escherichia coli</i> called DH5α and C41(DE3) to transform recombinant plasmids - pET21b (+). It carries the protein-coding sequences to produce the protein chimeras. Both of the <i>E.coli</i> strains that we choose have no harm on humans nor the natural environment. As PETase is toxic to E.coli, using the strain C41(DE3) allows the expression of toxic proteins, so that our protein chimeras can be expressed. <br> |
The chimeric proteins are then extracted and purified for use. Thus no genetically engineered bacteria are released into the environment. | The chimeric proteins are then extracted and purified for use. Thus no genetically engineered bacteria are released into the environment. | ||
</p> | </p> |
Revision as of 07:52, 13 October 2021