Difference between revisions of "Team:HK GTC/Parts"

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        <h2>Overview</h2>
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        <p>We created 4 basic parts and 2 composite parts.</p>
 
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         <h2> 1. BBa_K2982000 </h2>
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         <h2> 1. BBa_K3787001 </h2>
 
         <p> Wild Type PETase <br>
 
         <p> Wild Type PETase <br>
 
The coding sequence for wild type PETase, codon optimised for the gene expression in E. coli. It serves as a standard for comparison of performance of PETase mutants. <br></p>
 
The coding sequence for wild type PETase, codon optimised for the gene expression in E. coli. It serves as a standard for comparison of performance of PETase mutants. <br></p>
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         <h2> 2. BBa_K3787001 </h2>
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         <h2> BBa_K3787002 </h2>
 
         <p> PETase(WT)-MHETase chimera <br>
 
         <p> PETase(WT)-MHETase chimera <br>
 
         Composite part (Coding) <br>
 
         Composite part (Coding) <br>
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         The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.<br>
 
         The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.<br>
 
         It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker. </p>
 
         It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker. </p>
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        <h2>BBa_K3787003</h2>
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        <p> I.s. MHETase <br>
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Basic part (Coding) <br>
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Length: 1809 bp <br>
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The coding sequence for MHETase, codon optimized for the gene expression in E. coli. <br>
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The sequence comes from the bacteria Ideonella sakaiensis. It is used in enzyme cocktail experiments.
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</p>
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        <h2>BBa_K3787004</h2>
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        <p> 12 amino acid serine glycine linker <br>
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Basic part (Coding)
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Length: 36 bp <br>
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The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.
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Revision as of 05:42, 13 October 2021

HK_GTC 2021 Homepage

Parts

Overview

We created 4 basic parts and 2 composite parts.

1. BBa_K3787001

Wild Type PETase
The coding sequence for wild type PETase, codon optimised for the gene expression in E. coli. It serves as a standard for comparison of performance of PETase mutants.

BBa_K3787002

PETase(WT)-MHETase chimera
Composite part (Coding)
Length: 2715 bp
The coding sequence for PETase (wild type) and MHETase chimera, codon optimized for the gene expression in E. coli.
It is one of our chimeric proteins created, by linking wild type PETase and MHETase using a 12 amino acid serine glycine linker.

BBa_K3787003

I.s. MHETase
Basic part (Coding)
Length: 1809 bp
The coding sequence for MHETase, codon optimized for the gene expression in E. coli.
The sequence comes from the bacteria Ideonella sakaiensis. It is used in enzyme cocktail experiments.

BBa_K3787004

12 amino acid serine glycine linker
Basic part (Coding) Length: 36 bp
The coding sequence for the 12 amino acid serine glycine linker, used to link PETase and MHETase to form chimeric proteins.