Difference between revisions of "Team:Shanghai Metro Utd/Safety"

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<h1> Safety </h1>
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<p>Please visit the <a href="https://2021.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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    <title>Shanghai_Metro_Utd</title>
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_Metro_Utd/Main_CSS&action=raw&ctype=text/css" />
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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    <nav class="head-nav clearfix">
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        <div class="top-block"></div>
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        <div class="top-nav-bar">
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            <ul class="clearfix">
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                <span class="small-logo"></span>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_Metro_Utd">Home</a>
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                </li>
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                <li class="active">
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                    <a href="">Project</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Description"
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                                    class="sub-nav-74">Description</a></li>
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                </li>
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                <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Experiments"
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                        class="sub-nav-74">Experiments</a></li>
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                <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Results" class="sub-nav-74">Results</a></li>
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                <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/ProofOfConcept" class="sub-nav-52">Proof Of
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                        Concept</a></li>
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                <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Notebook" class="sub-nav-52">Notebook</a>
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                </li>
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                <li class="current-sub-nav"><a href="#">Safety</a></li>
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            </ul>
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        <li>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Collection" class="sub-nav-74">Parts
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                            Collection</a></li>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Engineering"
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                            class="sub-nav-74">Engineering</a></li>
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            <a href="">Human Practices</a>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Integrated" class="sub-nav-74">Integrated
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                            Human Practice</a></li>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Communication"
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                            class="sub-nav-74">Communication</a></li>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Fundraising"
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                            class="sub-nav-74">Fundraising</a></li>
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                </ul>
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            <a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Implementation">Implementation</a>
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        </li>
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        <li>
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            <a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Entrepreneurship">Entrepreneurship</a>
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        </li>
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        <li>
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            <a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Model">Model</a>
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            <a href="">Team</a>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Members" class="sub-nav-74">Team
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                            Members</a></li>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Attributions"
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                            class="sub-nav-74">Attributions</a></li>
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                    <li><a href="https://2021.igem.org/Team:Shanghai_Metro_Utd/Collaborations"
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                            class="sub-nav-74">Collaborations</a></li>
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    <div class="sub-content">
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        <div class="sub-title">SAFETY</div>
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        <div class="article-title black-font">General Safety Training</div>
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        <div class="article-content">Our team is very careful and responsible for each and every experiment we do in the
 +
            lab throughout the process. Before the experiments, we were trained to be aware clearly of all the
 +
            information about the specific experiment and wear lab gowns, gloves, and masks in the laboratory to avoid
 +
            skin as well as eye contact with all chemicals. When we use any materials with health risk, we have a mentor
 +
            or our lab instructor to supervise the whole process. The culture cabinets and other equipment we use are
 +
            sterile and can guarantee the safety of the reagents and the safety of the users. We were asked to wear
 +
            high-temperature gloves when working with warming agents. And we can't eat any food and drink any beverage
 +
            in the lab. During the experiments, we will make sure to change the pipet tips whenever necessary, use clean
 +
            spoons, and place emphasis on safety and chemical hygiene at all times. After the experiments, our used
 +
            waste liquid and other waste reagents were thrown into the designated waste tank. In the meantime, we will
 +
            place everything back to its original place and avoid all chemical exposures prior to leaving the
 +
            laboratory. Of course, we also carefully sorted our experimental notes of the day for review and records for
 +
            the wiki use.</div>
 +
        <div class="article-content">There was a situation that we were making the agarose gel, one of us used a used
 +
            spoon so that we had to dispose of the whole gel to guarantee no contamination. Whenever this type of
 +
            incident happened, we will be always cooperative and rigorous.</div>
 +
        <div class="article-title black-font">Microorganism Safety</div>
 +
        <div class="article-content">Recipient cells are also called host cells. The recipient cells are prokaryotic
 +
            receptor cells (mostly E. coli), eukaryotic receptor cells (mostly yeast), animal cells, and insect cells
 +
            (actually eukaryotic receptor cells). Among the prokaryotic receptor cells, the most commonly used host cell
 +
            is Escherichia coli, as we used in our project, which was considered with little harm to human bodies. But
 +
            be on the safe side, whenever we transferred or cultivated E. coli, we ensured no exposure to the
 +
            environments unless inside the super clean bench and all related experiments were conducted under
 +
            supervision of our lab instructor. When cultivating E. coli, we added antibiotics to the culture medium and
 +
            placed them in a sterile incubator.</div>
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Revision as of 15:24, 26 September 2021

Shanghai_Metro_Utd

SAFETY
General Safety Training
Our team is very careful and responsible for each and every experiment we do in the lab throughout the process. Before the experiments, we were trained to be aware clearly of all the information about the specific experiment and wear lab gowns, gloves, and masks in the laboratory to avoid skin as well as eye contact with all chemicals. When we use any materials with health risk, we have a mentor or our lab instructor to supervise the whole process. The culture cabinets and other equipment we use are sterile and can guarantee the safety of the reagents and the safety of the users. We were asked to wear high-temperature gloves when working with warming agents. And we can't eat any food and drink any beverage in the lab. During the experiments, we will make sure to change the pipet tips whenever necessary, use clean spoons, and place emphasis on safety and chemical hygiene at all times. After the experiments, our used waste liquid and other waste reagents were thrown into the designated waste tank. In the meantime, we will place everything back to its original place and avoid all chemical exposures prior to leaving the laboratory. Of course, we also carefully sorted our experimental notes of the day for review and records for the wiki use.
There was a situation that we were making the agarose gel, one of us used a used spoon so that we had to dispose of the whole gel to guarantee no contamination. Whenever this type of incident happened, we will be always cooperative and rigorous.
Microorganism Safety
Recipient cells are also called host cells. The recipient cells are prokaryotic receptor cells (mostly E. coli), eukaryotic receptor cells (mostly yeast), animal cells, and insect cells (actually eukaryotic receptor cells). Among the prokaryotic receptor cells, the most commonly used host cell is Escherichia coli, as we used in our project, which was considered with little harm to human bodies. But be on the safe side, whenever we transferred or cultivated E. coli, we ensured no exposure to the environments unless inside the super clean bench and all related experiments were conducted under supervision of our lab instructor. When cultivating E. coli, we added antibiotics to the culture medium and placed them in a sterile incubator.