Difference between revisions of "Team:Shanghai high school/Safety"

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<link rel="stylesheet" type="text/css"
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      href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_high_school/StyleCSS&action=raw&ctype=text/css"/>
  
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<h1> Safety </h1>
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<div class="dr-oral-content">
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<p>Please visit the <a href="https://2021.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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    <!--navbar-->
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    <div class="navbar">
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        <a class="logo" href="https://2021.igem.org/Team:Shanghai_high_school">
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            <img src="https://static.igem.org/mediawiki/2021/7/7f/T--Shanghai_high_school--logo.png" alt="">
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        </a>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        <!--nav-->
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        <ul class="list">
 +
            <!--Team-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_high_school/Team">Team</a>
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                <div class="child-nav">
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                    <ul class="child-list">
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Team">Team Members</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Attributions">Attributions</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Collaborations">Collaboration</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Partnership">Partnership</a>
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                        </li>
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                    </ul>
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                </div>
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            </li>
  
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</div>
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            <!--Modeling-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_high_school/Model">Modeling</a>
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            </li>
  
 +
            <!--Entrepreneurship-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_high_school/Entrepreneurship">Entrepreneurship</a>
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            </li>
  
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<div class="column two_thirds_size">
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            <!--Implementation-->
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<h3>Safe Project Design</h3>
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Shanghai_high_school/Implementation">Implementation</a>
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            </li>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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            <!--Human Practice-->
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            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_high_school/Human_Practices">Human Practice</a>
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                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Human_Practices">Integrated Human
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                                Practice</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Communication">Communication</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Fundraising">Fundraising</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<ul>
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            <!--Parts-->
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<li>Choosing a non-pathogenic chassis</li>
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            <li class="item">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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                <a href="https://2021.igem.org/Team:Shanghai_high_school/PartsCollection">Parts</a>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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                <div class="child-nav">
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<li>Including an "induced lethality" or "kill-switch" device</li>
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                    <ul class="child-list">
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</ul>
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                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Parts_Collection">Parts
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                                Collection</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Engineering">Engineering</a>
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                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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</div>
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            <!--Project-->
 +
            <li class="item active">
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                <a href="https://2021.igem.org/Team:Shanghai_high_school/Description">Project</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Description">Description</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Experiments">Experiments</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Results">Result</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/ProofOfConcept">Proof of Concept</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Notebook">Notebook</a>
 +
                        </li>
 +
                        <li class="child-item active">
 +
                            <a href="https://2021.igem.org/Team:Shanghai_high_school/Safety">Safety</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<div class="column third_size">
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            <!--Home-->
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<h3>Safe Lab Work</h3>
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            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Shanghai_high_school">Home</a>
 +
            </li>
 +
        </ul>
 +
    </div>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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    <!--图片-->
 +
    <div class="page-header">
 +
        <img src="https://static.igem.org/mediawiki/2021/f/fd/T--Shanghai_high_school--bg_1.png" alt="">
 +
        <h1>Safety</h1>
 +
    </div>
  
 +
    <!--内容-->
 +
    <div class="content-small">
 +
        <section class="article p-t-54 p-b-54">
 +
            <section>
 +
                <h1 class="title">General Safety</h1>
 +
                <p>Our lab at ATLATL is classified as Biosafety Level 1, which means the microbes here are not to known
 +
                    consistently cause disease in healthy adults and possess minimal potential hazard to laboratorians and
 +
                    the environment. The laboratory has complete medical waste treatment system and ultraviolet disinfection
 +
                    system. It can be guaranteed that it will not cause any harm to the laboratorians and the environment.
 +
                    The strains we use are safe and common laboratory strains, <i>Escherichia coli</i> (<i>E. coli</i>) K-12
 +
                    DH5α. Our lab has many rules and procedures used to regulate laboratory operations. For example, we
 +
                    prohibit food, drinks, shorts and open-toed slippers. In addition, we must wear masks, lab coats, and
 +
                    rubber gloves when we conduct experiments every day. The laboratory equipment we use (beakers, conical
 +
                    flasks, pipettes, test tubes) are sterilized by high temperature every day. Laboratory equipment that
 +
                    has been exposed to strains will be placed in a dedicated medical waste bucket for centralized disposal.
 +
                    In summary, our laboratory is harmless to both the lab’s staff and the environment.</p>
 +
            </section>
  
 +
            <section>
 +
                <h1 class="title">Project-Specific Safety</h1>
 +
                <p>Although our experimental design focused on oral vaccines for rotavirus, we did not use actual viruses.
 +
                    Instead, we used <i>Escherichia coli</i> and <i>Bacillus subtilis</i> as carriers, and verified whether
 +
                    they can carry rotavirus antigen proteins into the human body through experimental procedures such as
 +
                    strain culture and DNA replication. Because both <i>Escherichia coli</i> and <i>Bacillus subtilis</i>
 +
                    are common safe laboratory strains, this project is very safe and harmless.</p>
 +
            </section>
  
 +
            <section>
 +
                <h1 class="title">References</h1>
 +
                <ol>
 +
                    <li>
 +
                        <p>(n.d.). Retrieved from
 +
                            <u><a href="https://plasmid.med.harvard.edu/PLASMID/GetVectorDetail.do?vectorid=319">https://plasmid.med.harvard.edu/PLASMID/GetVectorDetail.do?vectorid=319</a></u>
 +
                        </p>
 +
                    </li>
 +
                    <li>
 +
                        <p>CDC LC Quick Learn: Recognize the four Biosafety Levels. (n.d.). Retrieved from</p>
 +
                    </li>
 +
                    <li>
 +
                        <p>
 +
                            <u><a href="https://www.cdc.gov/training/QuickLearns/biosafety">https://www.cdc.gov/training/QuickLearns/biosafety</a></u>
 +
                        </p>
 +
                    </li>
 +
                </ol>
 +
            </section>
 +
        </section>
 +
    </div>
 +
 +
 +
    <!--Footer-->
 +
    <div class="footer">
 +
        <div class="content">
 +
            <hr>
 +
            <span>Contact Email: 1649407718@qq.com; 2796437537@qq.com</span>
 +
            <span>Wechat Search ‘Dr Oral’ and follow us!</span>
 +
        </div>
 +
    </div>
 +
</div>
 
</html>
 
</html>

Revision as of 09:21, 23 September 2021

Safety

General Safety

Our lab at ATLATL is classified as Biosafety Level 1, which means the microbes here are not to known consistently cause disease in healthy adults and possess minimal potential hazard to laboratorians and the environment. The laboratory has complete medical waste treatment system and ultraviolet disinfection system. It can be guaranteed that it will not cause any harm to the laboratorians and the environment. The strains we use are safe and common laboratory strains, Escherichia coli (E. coli) K-12 DH5α. Our lab has many rules and procedures used to regulate laboratory operations. For example, we prohibit food, drinks, shorts and open-toed slippers. In addition, we must wear masks, lab coats, and rubber gloves when we conduct experiments every day. The laboratory equipment we use (beakers, conical flasks, pipettes, test tubes) are sterilized by high temperature every day. Laboratory equipment that has been exposed to strains will be placed in a dedicated medical waste bucket for centralized disposal. In summary, our laboratory is harmless to both the lab’s staff and the environment.

Project-Specific Safety

Although our experimental design focused on oral vaccines for rotavirus, we did not use actual viruses. Instead, we used Escherichia coli and Bacillus subtilis as carriers, and verified whether they can carry rotavirus antigen proteins into the human body through experimental procedures such as strain culture and DNA replication. Because both Escherichia coli and Bacillus subtilis are common safe laboratory strains, this project is very safe and harmless.

References

  1. (n.d.). Retrieved from https://plasmid.med.harvard.edu/PLASMID/GetVectorDetail.do?vectorid=319

  2. CDC LC Quick Learn: Recognize the four Biosafety Levels. (n.d.). Retrieved from

  3. https://www.cdc.gov/training/QuickLearns/biosafety

Contact Email: 1649407718@qq.com; 2796437537@qq.com Wechat Search ‘Dr Oral’ and follow us!