Jonas232005 (Talk | contribs) |
Jonas232005 (Talk | contribs) |
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</center> | </center> | ||
<center><img src="https://static.igem.org/mediawiki/2021/d/de/T--HK_GTC--fig13b.jpg"> | <center><img src="https://static.igem.org/mediawiki/2021/d/de/T--HK_GTC--fig13b.jpg"> | ||
− | <p>Figure 13b. PET film after incubation with 4μg | + | <p>Figure 13b. PET film after incubation with 4μg WT PETase and 8μg MHETase |
</center> | </center> | ||
<center><img src="https://static.igem.org/mediawiki/2021/1/12/T--HK_GTC--fig13c.jpg"> | <center><img src="https://static.igem.org/mediawiki/2021/1/12/T--HK_GTC--fig13c.jpg"> | ||
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<div class="single-image-with-desc"> | <div class="single-image-with-desc"> | ||
<center><img src="https://static.igem.org/mediawiki/2021/b/b5/T--HK_GTC--ex_2_1.png" alt=""></center> | <center><img src="https://static.igem.org/mediawiki/2021/b/b5/T--HK_GTC--ex_2_1.png" alt=""></center> | ||
+ | <p> Figure 16. SDS-PAGE of purified WT PETase, S245I PETase and WT PET-MHETase</p> | ||
</div> | </div> | ||
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<p>To optimize the condition for protein expression, we changed the induction condition in which the | <p>To optimize the condition for protein expression, we changed the induction condition in which the | ||
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<div class="single-image-with-desc"> | <div class="single-image-with-desc"> | ||
− | <center><img src="https://static.igem.org/mediawiki/2021/ | + | <center><img src="https://static.igem.org/mediawiki/2021/5/51/T--HK_GTC--ex_2_2.png" alt=""></center> |
<p>Figure 17. SDS-PAGE of purified WT PETase, S245I PETase, MHETase, WT PET-MHETase and | <p>Figure 17. SDS-PAGE of purified WT PETase, S245I PETase, MHETase, WT PET-MHETase and | ||
S245I PETase-MHETase. Arrows showing the correct size of new constructs, MHETase, | S245I PETase-MHETase. Arrows showing the correct size of new constructs, MHETase, |
Revision as of 11:07, 18 October 2021