Difference between revisions of "Team:IISER-Tirupati India/Communication"

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                                 <li><a class="dropdown-item" href="https://2021.igem.org/Team:IISER-Tirupati_India/Description">DESCRIPTION</a></li>
 
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                                 <li><a class="dropdown-item" href="https://2021.igem.org/Team:IISER-Tirupati_India/Engineering">ENGINEERING SUCCESS &nbsp; &nbsp;</a></li>
 
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                                 <li><a class="dropdown-item" href="https://2021.igem.org/Team:IISER-Tirupati_India/Communication">COMMUNICATION</a></li>
 
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                             </ul>
 
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                             TEAM
 
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                    <div class="col-md-4 col-lg-3 p-2 d-none d-md-block" style="background-color: #FFBD59 !important; color: #8D1063;">
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                        <div class="card" style=" max-width: 15.5rem; border: none !important;background-color:#FFBD59;" id="index">
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                                <h3 class="card-title text-center mb-3">INDEX</h3>
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                                <section id="Index1">
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                                    <ul>
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                                        <li><a class="index_link" href="#1">Introduction</a></li>
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                                        <li><a class="index_link" href="#2">Gene Gala</a></li>
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                                        <li><a class="index_link" href="#3">Syn Track</a></li>
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                                        <li><a class="index_link" href="#4">Knowledgehub session</a></li>
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                                        <li><a class="index_link" href="#5">Handbook of Biotechnology Laws in India</a></li>
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                                        <li><a class="index_link" href="#6">Interactive workshop with Agasthya</a></li>
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                                        <li><a class="index_link" href="#7">International survey on GMO</a></li>
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                                        <li><a class="index_link" href="#8">Interview with iGEM Ambassador</a></li>
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                                        <li><a class="index_link" href="#9">Guest Talk at Synteraction</a></li>
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                                        <li><a class="index_link" href="#10">Session with Synbio enthusiasts</a></li>
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                                        <li><a class="index_link" href="#11">Publishing an article in the Manthan Magazine</a></li>
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                                    </ul>                     
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                                </section>
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                        <h3 class="text-center p-3"><strong>&ldquo;Science is not finished unless it is communicated.&rdquo;</strong></h3>
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                        <p id="1"></p>
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                        <h4 class="p-3" style="text-align: end;">Mark Walport</h4>
 
                         <h2>Introduction</h2>
 
                         <h2>Introduction</h2>
                         <p>Natural systems are highly complex to understand as well as to experiment with. To make predictions of the outcomes,
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                         <p><strong><em>Communicating science is as important as science as itself. It can help spark curiosity and build a scientifically literate community. Through our outreach activities, we wanted to connect with people from different backgrounds and levels of expertise in synthetic biology and foster discussion on science and synthetic biology.&nbsp;</em></strong></p>
                            we use the available information and the knowledge of physics, mathematics, chemistry, and computer science to build a theoretical model. This page deals with the models built on the different aspects of our project namely :</p>
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                        <h2>OUR GOALS</h2>
                             <ol>
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                        <ul>
                                <li>GMO delivery</li>
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                             <li>Help people appreciate the concept of synthetic biology and how it can be applied to solve local or global problems.</li>
                                <li>GMO growth and colonisation</li>
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                            <li>Understand the public perception of synthetic biology</li>
                                <li>Protease production</li>
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                            <li>Bridge the gender gap in STEM and encourage girl students to pursue higher education in the STEM field</li>
                                <li>Diffusion and ovum Hardening</li>
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                            <li>Promote responsible research among the scientific community</li>
                                <li>Kill switch for reversibility</li>
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                            <li>Encourage students to learn about synthetic biology and pursue synthetic biology for their higher education.</li>
                                <li>Kill switch for Biosafety</li>
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                            <li>Encourage synthetic biology enthusiasts to participate in iGEM and develop innovative solutions to different challenges.</li>
                                </ol>
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                            <li>Communicate our idea to the public.</li>
                            <h2>Delivery & Colonisation</h2><br>
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                        </ul>
                            <h3>INTRODUCTION: THE JOURNEY OF GMO BEGINS</h3><br>
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                        <p>Over the course of iGEM we engaged in various outreach activities to realise our goals and create a positive impact on the community.&nbsp;</p>
                             <h4>PART A: Delivery</h4><br>
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                        <h2>HOW DID OUR WORK IMPACT THE SOCIETY? BOX&nbsp;</strong></h2>
                             <h5>OVERVIEW</h5>
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                        <ul>
                             <p>Delivery is an essential part of our project as it determines the audience we reach. We tried to develop multiple
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                             <li>We inspired high school girls to develop innovative solutions to real-world problems using synthetic biology and encouraged them to pursue STEM for higher education through the &ldquo;Gene Gala&rdquo;.</li>
                                ways to deliver GMO in a user-friendly way with minimum invasion. Minimum invasion means it should not colonize
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                             <li>We facilitated discussions on synthetic biology among students through &ldquo;Gene ala&rdquo;, a session with students from Agastya Foundation, and a Guest Talk at Synteraction.</li>
                                the whole reproductive tract or reach the ovaries. The device should ensure bacterial colonization in the ampulla
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                             <li>We launched SynTrack, a one-of-a-kind podcast for beginners in synthetic biology discussing a wide range of topics to give them a holistic view of the subject.</li>
                                of the fallopian tube.
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                            <li>We encouraged students to develop innovative solutions using synthetic biology and participate in iGEM through knowledge hub sessions, interacting with synthetic biology enthusiasts from our Institute via our guest talk at Synteraction.</li>
                                One of the constraints that we faced is the high viscosity of the oviductal fluid.</p><P>
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                             <li>We drafted a Handbook on Biotechnology Laws of India for Researchers and future iGEMers which can be used as a quick guide to go through relevant laws in a language easily understood by a person who is not from a legal background.</li>
                                After calculating the time taken for delivery in each method, talking to a couple of IVF experts, and getting their
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                             <li id="2">We conducted a survey to understand the perception of synthetic biology and GMOs worldwide in collaboration with Team MSP-Maastricht, Team Aachen, and Team IISER Pune.</li>
                                inputs into it,we thought hysteroscopic techniques would be the best for our purpose. This method gives us an advantage
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                        </ul>
                                by delivering the bacteria directly into the ampulla region.</p>
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                        <p>While engaging with people from different walks of life and different perspectives, we learned to view synthetic biology in a different light, encouraging us to look at the bigger picture than as an isolated subject.</p>
                             <h5>DEVICE DESIGNING (How to deliver?)</h5>
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                        <h2>Gene Gala: Mini-Summer School</h2>
                             <p>GMO will be delivered directly to the ampulla by a process called Hysteroscopy. It’s done with medical assistance and requires
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                        <p><strong>12th -16th July 2021</strong> </h2>
                                constant X-Ray monitoring to ensure the catheter is inserted without any issues. The reason is that the Ostia and the Fallopian
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                        <p>Science, Technology, Engineering, and Math (STEM) fields across the globe are highly dominated by men, with women making only a small fraction of the workforce in STEM. The lack of representation of women in science and social conditioning often leads them to underestimate their aptitude and steer them away from STEM. Our team wanted to take the initiative to bridge this gender gap in STEM, and this led us to launch &ldquo;Gene Gala&rdquo;, a Mini- Summer School to introduce high school girls to Synthetic Biology and encourage them to pursue higher education in the STEM field. To actualise this idea, we collaborated with Team IISER Kolkata and the Directorate of Education, GNCT Delhi, to conduct the Mini-summer school over the course of 5 days. Gene Gala saw the registration of over 90 girl students studying in their final year of high school.</p>
                                tube are dynamic structures and that there is no chemical or significant physical difference between the ampulla and the other
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                        <p>Through Gene Gala, we wanted to provide a platform for girl students to cultivate a creative and problem-solving mindset and appreciate the concepts of Synthetic Biology. We utilised the initial four days to discuss the core concepts of synthetic biology, encourage them to participate in discussions, and communicate their ideas with their peers.&nbsp;</p>
                                components of the Fallopian tube.</p>
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                        <p>We discussed biomolecules that make up a cell, bacterial genetics and how they are modulated, molecular techniques for redesigning genetic circuits and ethical concerns associated with synthetic biology. After each session, we divided the students into breakout rooms where we conducted fun quizzes, activities, and puzzles to keep them engaged and promote discussions. On the final day of the workshop, we introduced them to some exciting iGEM projects and encouraged them to develop solutions of their own to real-world challenges using the recently learned concepts of Synthetic Biology. The students actively contributed to the discussions and came up with several interesting ideas for designing their own Genetically Modified Organism.</p>
                               
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                        <p>When conducting Gene Gala, we were met with the challenge of covering broad concepts in a short duration of time and facilitating discussions. We came up with an effective break-up of the curriculum to achieve this and conducted breakout sessions for discussions and activities.</p>
                            <p>A solution of GMO of a specific concentration is prepared. Then the catheter is inserted under medical guidance up to the ampulla.  
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                        <p>We encouraged them further to pursue STEM for higher education and provided the details on opportunities of Higher education in STEM in India, relevant scholarships, and the career paths in STEM through a day-wise Handbook which was provided to the students. We received very positive feedback from the students who participated in the Mini-summer school for inspiring them and giving them a sneak-peak into the world of Synbio and its opportunities.</p>
                                Then an injector is projected with narrow long incisions on both sides of it, which ensures that the GMO forms a ring along the  
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                        <p>We have documented our experience in conducting Gene Gala and have elaborately detailed our strategies for designing the curriculum, conducting the activity session and designing the materials used, which can be used by future iGEM teams when conducting similar workshops.</p>
                                circumference of the tube. Ovum is now
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                        <p>Click here on button to download the PDF file.</p>
                                360° surrounded by the GMO. </p>
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                        <a class="btn btn-primary" href="PDFLINK" download><i class="fa fa-file-pdf-o" aria-hidden="true"></i>Download</a></li>
                               
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                        <figure class="col-12 col-md-8 p-5 m-auto">
                            <p>The GMO Lactobacillus Acidophilus has pili which expectantly forms hydrogen bonds at the tube circumference with mucin found in mucus.  
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                            <img src="./assets/Progesterone (nM) vs. Day.png" alt="Trulli" style="width:120%">
                                The GMO is now adhered to the walls and multiplies once it adjusts to the introduced environment (lag phase) </p>
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                        </figure>
                            <h5>FUTURE ASPECTS:</h5>
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                        <figure class="col-12 col-md-8 p-5 m-auto">
                            <p>Since we aim to reach a larger population, the hysteroscopic technique, we believe, is too expensive.  
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                             <img src="./assets/Progesterone (nM) vs. Day.png" alt="Trulli" style="width:120%">
                                We also considered using hydrogels for the delivery of bacteria at a pH-specific region.</p><P>
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                        </figure>
                                Why is this system compatible with the delivery of bacteria in the ampulla of the Fallopian tube region?</P><P>
+
                        <h3>Resources and Educational Tools</h3>
                                We found out that the pH in the oviductal Ampulla region is close to 8.0.
+
                        <ol>
                                That’s why we considered using hydrogels for bacterial delivery, which swells up at a pH range of 7.8 to 8.0. </P><P>
+
                             <li><a style="color: #8D1063;" target="_blank" href="https://static.igem.org/mediawiki/2021/0/01/T--IISER-Tirupati_India--Agarose_gel_electrophoresis.pdf">
                               
+
                                 <i class="fa fa-file-pdf-o" aria-hidden="true"></i>Lesson Plan</a></li>
                                Amongst the stimuli-responsive hydrogels, pH-sensitive hydrogels are the most studied hydrogels. The rate at
+
                             <li><a style="color: #8D1063;" target="_blank" href="https://static.igem.org/mediawiki/2021/0/01/T--IISER-Tirupati_India--Agarose_gel_electrophoresis.pdf">
                                which hydrogels respond depends upon their size, shape, cross-linking density, number of ionic groups, and composition,  
+
                                 <i class="fa fa-file-pdf-o" aria-hidden="true"></i>Class Material</a></li>
                                which can be tailored by varying these factors. The response rate increases with increasing pore size and number of ionic
+
                            <li><a style="color: #8D1063;" target="_blank" href="https://static.igem.org/mediawiki/2021/0/01/T--IISER-Tirupati_India--Agarose_gel_electrophoresis.pdf">
                                groups and decreasing their size and cross-linking density.</P><P>
+
                                 <i class="fa fa-file-pdf-o" aria-hidden="true"></i>Handbook</a></li>
                               
+
                            <li id="3"><a style="color: #8D1063;" target="_blank" href="https://static.igem.org/mediawiki/2021/0/01/T--IISER-Tirupati_India--Agarose_gel_electrophoresis.pdf">
                                For a delivery in a basic medium, we planned to use anionic hydrogels, such as carboxymethyl chitosan, which swell at higher
+
                                 <i class="fa fa-file-pdf-o" aria-hidden="true"></i>Gene Gala</a></li>
                                pH (basic medium) due to ionization of the acidic groups. As a result, the ionized negatively charged pendant groups on the
+
                        </ol>
                                polymer chains cause repulsion leading to swelling. This property of hydrogels can be exploited for GMO delivery at pH 7.4
+
 
                                in the ampulla of the fallopian tube. </p>
+
                        <p >See <a  href="https://2021.igem.org/Team:IISER-Tiruphttps://2021.igem.org/Team:IISER-Tiruphttps://2021.igem.org/Team:IISER-Tirupati_India/Contributionati_India/Contributionati_India/Contribution">Contributions</a> page for the Resources and Educational tools.&nbsp;</p>
                             <h5>CELL ENCAPSULATION (What to deliver?)</h5>  
+
 
                             <p>Our goal is to design a live-bacteria entrapment system. More than just encapsulating bacteria, we want to entirely prevent
+
                        <h2>SynTrack: The Podcast Series</h2>
                                 their escape from the bead body into the surroundings.</p>
+
                        <p>We are a diverse team with people from different disciplines united by our shared passion for biology, and we were thrilled to share our excitement and curiosity about synthetic biology with people outside of our iGEM Team. This shared interest led us to launch SynTrack, a one-of-a-kind podcast launched for science enthusiasts who have just begun to explore the realms of Synbio. The podcast tries to capture the different flavours of the Synbio, ranging from experiments in the laboratory to opportunities in entrepreneurship, potential applications and ethical and moral implications of using genetic engineering. The podcast follows a casual conversational style podcast guiding the listeners through various aspects of Synbio as they get on with their everyday tasks. SynTrack provides an opportunity for the listeners to look at synthetic biology in a positive light and appreciate the potential of Synthetic biology as a powerful tool capable of revolutionising the landscapes of Health, Agriculture, Environment, and Bioenergy. The podcast included episodes that were recorded in collaboration with Spoorthy Gowda (Student at IISER Tirupati), Team Groningen, Team FCB- UANL, Team Ioannina, and TU Delft. The podcast was recorded over Zoom or Gmeet and launched on Spotify and Google Podcast.</p>
                             <p>The Oviductal Fluid is slightly alkaline (ph 7.4 to 7.7), so we need our encapsulating membrane to dissolve away at this pH.  
+
                        <p>The podcast was also translated into Hindi, a language widely spoken in India, and made available on Spotify. The podcast is beneficial for the local population who have better command over Hindi than English. Hindi is not a popularly used language for science communication. Launching the translated version of the podcast allowed us to tap into a broad audience of listeners and provided us with an opportunity to connect with listeners and communicate science crossing the language barriers.</p>
                                 Potential candidates for hydrogel materials include chitosan, guar gum, and xanthan. Chitosan forms Hydrogen bonds with the
+
                        <figure class="col-12 col-md-8 p-5 m-auto">
                                 mucin protein in the mucus allowing for the anchoring at the walls and preventing the hydrogels from getting washed away.</p>
+
                             <img src="./assets/Progesterone (nM)  vs. Day.png" alt="Trulli" style="width:120%">
                               
+
                        </figure>
                                  
+
 
                               
+
                        <h3  id="4">Resources</h3>
                            <h4>PART B: Colonization</h4><br>
+
                        <ul>
                            <h5>OVERVIEW (why is it necessary to study growth and colonization)</h5>  
+
                        <li><a href="https://open.spotify.com/show/1ZGocNKdxmCReQbIuD3zVG?si=43fbd5d4fed74dfe">SynTrack</a></li>
                            <p>In biosynthesis, growth kinetics is a crucial study to be conducted. The growth of a cell comprises both the size and the number.
+
                        <li ><a href="https://open.spotify.com/show/4iVd0Kcu2jFp8mPw6fh1S7?si=49cd740d2a12438f">SynTrack | Hindi </a></li>
                                These growths are affected by external factors such as temperature, the chemical composition of the growth nutrient, etc., and by
+
                        </ul>
                                different physiological factors such as growth factor proteins[1][2]. The cells in a particular environment extract the nutrients
+
 
                                from the growth media and produce biomolecules, which humans utilize for different purposes. This phenomenon has applications, from
+
                        <h2>Let&rsquo;s talk about iGEM: A Knowledge Hub session with Next Gen Scientists Foundation&nbsp;</h2>
                                producing delicious Italian wine to getting antibiotics which saves millions of lives every year. We will be using this simple
+
                        <p><strong>29 Aug 2021</strong></p>
                                formula to reach the goals of our project. To have a qualitative understanding of the protein production by the Genetically Modified
+
                        <p>iGEM has contributed significantly to foster innovation in synthetic biology among the student community, and the number of iGEM has increased over the years. However, we noticed that the number of teams participating from Asia in the competition is significantly lower when compared to its western counterparts from Europe and North America. We wanted to encourage students in India to participate in iGEM, which led us to collaborate with the Next Gen Scientists Foundation to launch an initiative, &ldquo;Let&rsquo;s talk about iGEM&rdquo;, to reach out to other STEM students and Synthetic biology enthusiasts across the nation.&nbsp;</p>
                                anism(GMO), we need to have a theoretical approach. We need to understand how we can calculate the growth of GMOs [3]. This, in turn,  
+
                        <p>The Next Gen Scientists Foundation is an organisation governed by a student body from students across India to connect all Indian Life Science Researchers. In our collaboration with them, our team hosted a Knowledge Hub session via their discord server called the Indian Life Science Network. This session connected us to students across the nation with interests in Synthetic Biology. The session was further made available via Youtube to make it more accessible to a larger audience. We invited Ms Shruti Shridhar, iGEM Ambassador Asia, as the speaker for the event to talk about the competition. Through the Knowledge Hub session, Shruti introduced the audience to the iGEM competition and discussed the values of iGEM. The session was conducted as a conversation between an iGEM ambassador and one of our team members like a live podcast, providing the audience with the provision to ask their questions and get them answered then and there.</p>
                                will help us figure out protein production. This detailed study of growth kinetics will help us calculate the initial inoculation
+
                        <p>The session guided the audience on what iGEM is, the multidisciplinary nature of the iGEM projects, and how iGEM fosters advancements in synthetic biology. It further navigated the audience through the process of iGEM, covering various aspects of the projects from Team Building, Funding, Collaborations, Human Practices, and the time frame of the competition. Ms Shruti also talked about the challenges faced by iGEM teams during the pandemic and how to manage and tackle them. She also encouraged the audience to avail the iGEM mentorship programme, which allows the iGEM team to connect with mentors to guide them on their iGEM journey. The session gave all-around information to Synthetic Biology Enthusiasts about the iGEM competition and how to navigate through the iGEM journey.</p>
                                required for the production of a protein that we need.</p>
+
                        <p>Insert Image of the poster</p>
                            <p>For a better understanding, we will be considering two cases. The first comprises the study of the growth of Bacillus Subtilis in
+
 
                                normal petri dish conditions. This is the known environment and we can have this condition easily in computer simulation and/or lab
+
                        <h3 id="5">Resources</h3>
                                and reconfirm our model. The second case of study is the continuous culture model where we study the growth of Lactobacillus
+
                        <ul>
                                Acidophilus in the fallopian tube, which is our target region. </p>
+
                            <li><a href="https://youtu.be/S7gCRj2dFVM">Let&rsquo;s talk about iGEM: Knowledge Hub Session</a></li>
                           
+
                        </ul>
                           
+
 
                             <h6>GROWTH MODELS</h6><br>
+
                        <h2>The Handbook of Biotechnology laws in India&nbsp;</h2>
                            <p>PETRI DISH MODEL (Bacillus Subtilis, curve fitting)</p><br>
+
                        <p>When working on the project, we realised the importance of biosafety when designing and performing experiments. This motivated us to look at the legal framework regulating biotechnology research in India. In this process, we realised that the biotechnology laws are scattered throughout The Constitution of India, which makes it difficult for people without legal knowledge to understand the legal protocols and refer to relevant laws. This inspired us to draft a handbook to guide the researchers through the relevant laws in Biotechnology that they might have to go through before their research. The book compiles relevant biotechnology laws in India and Multilateral legal agreements of which India is a part of. It attempts to capture the multiple facets of law that must be abided by while performing scientific experiments in biotechnology in one place. When reading the various biotechnology laws, we also recognised some gaps in the legal framework, which motivated us to incorporate a section on gaps and unattended problems in the legal architecture into the Handbook. We also added a section for future iGEM teams to refer to with regard to safety practices that must be followed over the course of their project. The book was launched with the aim to promote responsible research among the research community in India.&nbsp;</p>
                            <p>CONTINUOUS CULTURE MODEL (lactobacillus acidophilus + fallopian tube environment)</p><br>
+
 
                            <h5>INOCULUM CALCULATIONS (connection with 2nd module)</h5><br>
+
                        <p>In collaboration with Team IISER Thiruvananthapuram, our team identified important biotechnology laws, identified the relevant Biotechnology laws, and designed the framework of the Handbook. The book is subdivided into four chapters:</p>
                            <h2>Protease production</h2><br>
+
 
                            <h3>OVERVIEW ( overall picture) </h3>
+
                        <ul>
                            <p>Well known for extracellular protease production[1], Bacillus subtilis is our model organism for the proof of concept experiments. Moreover,  
+
                            <li>Animal experimentation and Clinical trials</li>
                                it is a gram-positive bacteria which even if not too close but is closer than e coli (gram-negative) to our proposed bacteria lactobacillus
+
                            <li>Export-Import Policies</li>
                                Acidophilus. This module deals with the whole mechanism of action ideally the GMO must follow for contraception to be attained. To know how
+
                            <li>Environmental Safety</li>
                            this module developed to what it is, please refer to the engineering success (hyperlink).</p>  
+
                            <li>Biocontainment and Biowarfare</li>
                            <p>Let's create the flow, you must know by now that we plan to produce the protease known as ovastacin,  
+
                        </ul>
                                an indigenous protease[2] to the human ovum. It is known to cause Zona pellucida hardening naturally
+
 
                                used by the ovum to prevent polyspermy[3]. To see the mechanism of zona pellucida hardening click here
+
                        <p id="6">During the drafting of the Handbook, we met with Dr Harilal Madhavan, a development economist and faculty at IISER Thiruvananthapuram; Miss Parvathy, a student from the National Law School of India University; and Dr Subha C C, a faculty at the Department of School of Law in Christ University, Bangalore who provided us valuable advise and feedback enabling us to revise and improve our Handbook.</p>
                                (goes to project overview where it is explained).</p>
+
 
                           
+
                        <h3>Resources</h3>
                           
+
 
                            <h5> 1] Total amount of ovastacin required: </h5>
+
                        <a>The Handbook of Biotechnology laws in India.</a>
                           
+
 
                            <p>[We assume one active ovastacin cleaves one ZP2 glycoprotein present in the Zona pellucida matrix. To get the number of molecules of ovastacin needed, we calculated the number of ZP2 glycoproteins present on the surface of the ovum. For this, we reached out to studies that looked at the thickness of the ovum with and without the zona pellucida layer to find its overall thickness and the radius of the ovum[4]. </p>
+
                        <h2>Interactive Workshop with Agastya Foundation&nbsp;</h2>
                            <br>
+
                        <p>When collaborating with iGEM Teams and reading the past iGEM projects, we were deeply awed by the possibilities of synthetic biology and its potential. This motivated us to reach out to students to introduce them to synthetic biology and encourage them to pursue Synbio for higher education. To realise this, we conducted a workshop collaborating with Agastya Foundation for students with a passion for Biology.</p>
                            <h6>Constants and calculations:</h6><br>
+
                        <p>Agastya Foundation is a non-profit organisation based in Bangalore, India, aiming to nurture creativity and curiosity through education to students from lower economic strata. During the session, we introduced them to the foundational concepts in synthetic biology and gave them a flavour of the multidisciplinary facets of synthetic biology. We further introduced them to iGEM to inspire them to participate in iGEM in future. Following this, we conducted a brainstorming session, during which we encouraged them to develop ideas based on synthetic biology for contraception. The students actively participated in the discussions and came up with creative solutions and questions about developing novel contraceptives. We further discussed with them the opportunities of STEM education in India and the relevant scholarships and internship opportunities to pursue STEM. We also introduced them to Ovicloak, discussing the biological concept behind the idea, our motivation and plans for implementation.&nbsp;</p>
                            <div class="table-responsive-md">
+
                        <figure class="col-12 col-md-8 p-5 m-auto">
                                <table class="table">
+
                            <img src="./assets/Progesterone (nM)  vs. Day.png" alt="Trulli" style="width:120%">
                                    <thead>
+
                        </figure>
                                        <tr>
+
                        <p id="7" class="py-5"></p>
                                          <th>Parameter (need alternative) </th>
+
                        <h2>International Survey on Genetically Modified Organisms</h2>
                                          <th>Value</th>
+
                        <p>Our team conducted an International survey in collaboration with Team MSP- Maastricht, Team Aachen, and Team IISER-Pune-India to understand the attitude of the public towards genetic engineering organisms and the applications of synthetic biology across the world. The survey received 259 responses from Asia and Europe and was conducted through Google Forms.&nbsp;</p>
                                          <th>References</th>
+
 
                                        </tr>
+
                        <p>The survey questions were designed to understand the perception towards synthetic biology and GMOs, how receptive are people towards the different applications of synthetic biology and understand the concerns people have about GMOs. The survey revealed that the people largely had a positive perception towards synthetic biology and GMOs while they also expressed their concerns about potential damage to the environment, potential harm to human health, potential misuse, and ethical concerns on the creation of artificial life. We also observed that the opinion towards GMOs varied depending on the context of the application. Our analysis also revealed that the general outlook towards synthetic biology and concerns towards its usage were strikingly similar in both Europe and Asia.<p id="8"></p> The results of the survey analysis are published on the iGEM wiki page, which would be useful for future iGEM teams to gain an insight into how receptive people will be towards synthetic biology and its various applications.</p>
                                    </thead>
+
 
                                    <tbody>
+
                        <h3>Resources</h3>
                                        <tr>
+
                        <a href="">International survey on GMO</a>
                                          <td>Zona pellucida thickness</td>
+
 
                                          <td>18.9 μm</td>
+
                        <h2>An Interview with iGEM Ambassador Asia</h2>
                                          <td>Does zona pellucida thickness influence the fertilization rate? E. Bertrand1 , M.Van Den Bergh and Y.Englert</td>
+
                        <p>One of our team members interviewed the iGEM Ambassador of Asia, Miss Shruti Shridhar, to write an article to be published in Next Gen Scientists Foundation (NGSF) Blog. NGSF is a community of life science researchers and students predominantly in India. During the interview conducted we discussed her iGEM experience, the journey to becoming the iGEM Ambassador to Asia, plans on expanding iGEM in India, and the challenges in introducing iGEM to Indian colleges and highschool.&nbsp;</p>
                                        </tr>
+
 
                                        <tr>
+
                        <p>While a large number of resources are available on the iGEM journey, only very few resources are available to know about opportunities and experience after iGEM, which motivated us to interview Miss Shruthi and publish a blog article.</p>
                                          <td>Oocyte diameter</td>
+
                        <p id="9">The Interview article would be helpful for current and future iGEMers to learn about opportunities after-iGEM. The article is currently in the process of being published in the NGSF Blog, which has viewers from over 73 different countries allowing us to reach out to students of the scientific community.</p>
                                          <td>123.5 μm</td>
+
 
                                          <td>Does zona pellucida thickness influence the fertilization rate? E. Bertrand1 , M.Van Den Bergh and Y.Englert</td>
+
 
                                        </tr>
+
                        <h2><strong>Guest Talk at Synteraction</strong></h2>
                                        <tr>
+
                        <p><strong>21 July 2021</strong></p>
                                          <td>Size of ZP2</td>
+
                        <p>Our team was invited to deliver a guest talk at Synteraction, a summer school programme launched by Team iGEM IIT Madras for their first year B.Tech students. Through the talk we wanted the audience to appreciate how synthetic biology can be employed to solve social challenges and how various disciplines intersect and overlap during the development of a project. During the talk, we introduced the audience to our project idea: Ovicloak - A Novel Contraceptive. We introduced the audience to the biological concepts on which the idea is based. We discussed with the audience about the engineering aspects of the idea and logic behind the genetic engineering circuits employed. We also discussed the importance of the kill switch in ensuring the biosafety aspect of our project and the mechanism behind the working of the kill switch. We discussed the multidisciplinary nature of the project and introduced the audience to the various facets of our project, such as genetic engineering, mathematical modelling, and human practices, to help them appreciate how the various disciplines of physics, mathematics, biology, and social science is integrated during the development of the project. We walked them through the importance of Human Practices of iGEM and how our team incorporated the iGEM values into our project. We explained how we engaged with the stakeholders and incorporated their feedback while developing the project. We further discussed the outreach activities launched by our team to engage with the public and their importance in initiating thoughtful discussions about the values related to our project. We also navigated the audience through our iGEM journey and how we tackled the challenges posed by the pandemic.&nbsp;</p>
                                          <td>90–110 kDa</td>
+
 
                                          <td>Characterization of human zona pellucida glycoproteins A R Bauskin 1, D R Franken, U Eberspaecher, P Donner DOI: 10.1093/molehr/5.6.534</td>
+
                        <figure class="col-12 col-md-8 p-5 m-auto">
                                        </tr>
+
                            <img src="./assets/Progesterone (nM)  vs. Day.png" alt="Trulli" style="width:120%">
                                        <tr>
+
                        </figure>
                                          <td>Diameter of ZP2r</td>
+
                        <p class="pt-5" id="10"></p>
                                          <td>0.0092 μm</td>
+
                        <h2><strong>A session with Synbio enthusiasts&nbsp;</strong></h2>
                                          <td>Zetasizer Nano Sensitivity Calculator (Classic)</td>
+
                        <p>Our team held an interactive session with the Synbio enthusiasts in our Institute to kickstart their iGEM journey. Through this session, we guided the potential future iGEMers from our Institute in planning and developing their project and addressed their queries regarding the various aspects of iGEM. During the interactive session, we walked them through our iGEM journey from team building to project development and the challenges we faced during the pandemic.<p id="11"></p> We discussed the multidisciplinary nature of the project and building a diverse team with different fields of expertise to help look at the problem from different perspectives. We also discussed the timeline of the iGEM Project to help them plan their iGEM journey. The importance of identifying the stakeholders and engaging with them to integrate their feedbacks while developing the project was also discussed, and some initial guidelines for the funding aspect of the project were also provided.</p>
                                        </tr>
+
                        <h2><strong>Publishing an article in the Manthan magazine&nbsp;</strong></h2>
                                    </tbody>
+
                        <p>Our team published an article in the Manthan magazine, a student magazine launched by the students of the Indian Institute of Science Education and Research, Mohali, to promote our project Ovicloak among the student community. Through the article, we wanted to provide an overview of our project to the student community across India. We used this opportunity to discuss the goals envisioned by our team, the hurdles we faced during its development, the progress we made, and the impact our project could have on the world around us. We communicated to the readers how Ovicloak has the potential to address issues related to reproductive health, family planning, inclusivity, and ecological crises on both the global and local scales. We also emphasised how Ovicloak was designed to make the contraceptive inclusive for all uterus owners as compared to alternative options available in the market which are not inclusive for individuals with different hormonal levels, reproductive tract disorders, fertile intersex and trans individuals. We also discussed through the article how Ovicloak could be a safer alternative with respect to the health of contraceptive users and the environment at large.&nbsp;</p>
                                </table>
+
 
                            </div>                          
+
                        <h3><strong>Resources</strong></h3>
                            <p>Assuming cuboid with all spheres of size same as ZP2 to  
+
                        <a href="https://manthanmagazine.wordpress.com/2021/10/03/ovicloak-a-novel-contraceptive-for-uterus-owners/">Manthan article</a>
                            get volume of one unit = 3.1 e-6 μm3 </p>
+
 
                            <p>Volume occupied by whole ZP matrix = 12.4 e5 μm3 </p>
+
                        <h2><strong>DNA Day with MolecularCloud</strong></h2>
                            <p>Calculated the number of ZP2 glycoproteins present in the ZP matrix = 3.92 x 1011 molecules</p>
+
                        <p>The Discovery of DNA is an important milestone in the history of genetic research. Elucidation of the structure of the DNA by James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin and colleagues led to the subsequent development in molecular biology and genetic engineering. To celebrate and commemorate this success, three of our team members created an artistic representation of DNA.&nbsp;</p>
                            <p>Assuming 1 ovastacin cleaves 1 ZP2</p>
+
 
                            <p>No. of ovastacin = No. of ZP2 = 12.4 e5 / 3.1 e-6 = 3.92 x 1011  molecules ]</p>
+
                        <p>Our team member, Shivam Kumar, designed a pencil sketch titled &ldquo;ASTRA DNA&rdquo;, which represents an amalgamation of biology and astronomy depicting the advancements and developments made in the macro and micro world. The second artwork was created by Lochan Chaudhari titled &ldquo;DNA dancing to the tune of life&rdquo;, which represents the dancing DNA being transcribed to give rise to the melody of life. Komal Pati created the third artwork titled &ldquo;The secret of life&rdquo;, illustrating how DNA lays the foundation of life, leading to the diverse creatures we see today. These images were published by MolecularCloud on their website for the DNA Day Photo Contest of which <strong>ASTRA DNA</strong> and <strong>DNA dancing to the tune of life</strong> received Silver and Bronze prize and <strong>The secret of life</strong> received an honourable mention. The images were also published in our social media handle to communicate to a broader audience.</p>
                            <p>In order to cause complete hardening __ number of molecules are required to reach the ovum. The next question that arises is how much is produced and how much of produced ovastacin will reach the ovum. So we have two major things left to look at: </p>
+
                        <figure class="col-12 col-md-8 p-5 m-auto">
                            <h5>2] Production and transport of ovastacin by GMO</h5>  
+
                            <img src="./assets/Progesterone (nM)  vs. Day.png" alt="Trulli" style="width:120%">
                            <p>The production of ovastacin is required for three days pre and post ovulation, please go to “Genetic Circuits” to learn details regarding the genetic circuit. </p>  
+
                        </figure>
                            <h4>GENETIC CIRCUIT</h4>
+
                        <h2><strong>Engaging with the public through social media&nbsp;</strong></h2>
                            <p>Progesterone repressible system</p>
+
                        <p>Our team engaged with the public through its social media handles, giving a sneak peek of our project work from time to time. To provide the audience with a short glimpse into our daily routine at the laboratory, we conducted Insta live demonstrating Agarose gel preparation and SDS gel preparation. To introduce our viewers to our idea- Ovicloak, we released our promotional video to communicate to a large audience about our project idea and how Ovicloak can be a safe and sustainable alternative to the currently available contraceptives. We subsequently also released the promotional video in Sign language to communicate to a more inclusive audience.&nbsp;</p>
                            <h4>JOURNEY OF OVASTACIN</h4>
+
 
                            <p>From the papers, we know that the ovum is propelled by the ciliary motion away from the uterus and that means the ovum is in contact with the wall. Thesize ampulla region of the Fallopian tube (2.5 mm ≤ radius ≤ 5 mm ) is much larger than the radius of the ovum (61.7μm) A molecule under the influence of Brownian motion move according to the equation</p>
+
                        <h3><strong>Resources</strong></h3>
                            <ul>
+
                        <p><a href="https://www.instagram.com/igem_iisertirupati/?hl=en">Instagram</a></p>
                                <li>< r <sup>2</sup> >= 4Dt (for 2-D)</li>
+
                        <p><a href="https://twitter.com/iiisert?lang=en">Twitter</a></p>
                                <li>< r <sup>2</sup> >= 6Dt (for 3-D)</li>
+
 
                                <p>Where,</p>
+
                        <h2><strong>Interview with Genscript</strong></h2>
                                <li>< r <sup>2</sup> > → mean squared (radial) distance travelled by the molecule</li>
+
                        <p>Our team was given an opportunity to be interviewed by Genscript to discuss Ovicloak and how we used synthetic biology to develop a contraceptive. The interview provided us with an opportunity to interact with professionals in the field of synthetic biology and gain public exposure. During the interview, we discussed our idea and the social impact of the project. We also discussed how we plan to incorporate Human Practices into our project. We also discussed our aim to instigate a fresh perspective on contraceptive development using synthetic biology. The recorded video of the session was uploaded on Youtube by Genscript, which provided us with an opportunity to communicate our idea to the scientific community.&nbsp;</p>
                                <p>using,</p>
+
 
                                <ol>
+
                        <a href="https://drive.google.com/file/d/1APfF7umxxTqoAq1yGJL2vqbl0z5Abi5c/view?usp=sharing">Genscript Interview</a>
                                    <li>k<sub>B</sub> = 1.38064852 ×10<sup>23</sup> m<sup>2</sup> kg<sup>-2</sup> s<sup>-1</sup> K  [Boltzmann constant]</li>
+
                                    <li>η = 0.799 Pa·s [Viscosity of Oviductal Fluid]</li>
+
                                    <li>T = 35.5 + 273.15 = 308.65 K [Temperature of Oviduct]</li>
+
                                    <li>r = 22kDa = 2.43 nm [Radius of ovastacin molecule]</li>
+
                                    <li>a = 61.7 μm [Radius of the ovum]</li>
+
                                    <li>s = 5 mm [Radius of the ampulla region of the oviduct]</li>
+
                                    <li>N<sub>0</sub> = 3.92 ×10<sup>11</sup> molecules = 0.000650946529392 nanomoles [Number of ovastacin to react with all ZP2]</li>
+
                                    <p>
+
                                        For, Ovastacin D=1.1644194699 ×10<sup>-13</sup>   m<sup>2</sup> s<sup>-1</sup>
+
                                        </p>
+
                                </ol>
+
                                <li>t → Time elapsed</li>
+
               
+
                            </ul>
+
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
                <div class="card d-none d-md-block" style="max-width: 18rem;width: 25%; border: none !important;" id="index">
 
                    <div class="card-body">
 
                      <h5 class="card-title">INDEX</h5>
 
                      <h6 class="card-subtitle mb-2 text-muted">Card subtitle</h6>
 
                      <p class="card-text">Some quick example text to build on the card title and make up the bulk of the card's content.</p>
 
                      <a href="#" class="card-link">Card link</a>
 
                      <a href="#" class="card-link">Another link</a>
 
                      <ul>
 
                          <li><a href="#waste1">waste 1</a></li>
 
                          <li><a href="#waste2">waste 2</a></li>
 
                          <li><a href="#waste3">waste 3</a></li>
 
                          <li><a href="#waste4">waste 4</a></li>
 
                          <li><a href="#waste1">waste 1</a></li>
 
                          <li><a href="#waste2">waste 2</a></li>
 
                          <li><a href="#waste3">waste 3</a></li>
 
                          <li><a href="#waste4">waste 4</a></li>
 
                          <li><a href="#waste1">waste 1</a></li>
 
                          <li><a href="#waste2">waste 2</a></li>
 
                          <li><a href="#waste3">waste 3</a></li>
 
                          <li><a href="#waste4">waste 4</a></li>
 
                      </ul>
 
                    </div>
 
                  </div>
 
 
             </div>
 
             </div>
 +
            <button type="button" class="btn button-dark btn-floating btn-lg" id="btn-back-to-top"><svg fill="#FDF8D7" width="20px" height="30px" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M34.9 289.5l-22.2-22.2c-9.4-9.4-9.4-24.6 0-33.9L207 39c9.4-9.4 24.6-9.4 33.9 0l194.3 194.3c9.4 9.4 9.4 24.6 0 33.9L413 289.4c-9.5 9.5-25 9.3-34.3-.4L264 168.6V456c0 13.3-10.7 24-24 24h-32c-13.3 0-24-10.7-24-24V168.6L69.2 289.1c-9.3 9.8-24.8 10-34.3.4z"/></svg></button>
 
         </main>
 
         </main>
  
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                         <h6 class="quick_link_heading">WETLAB</h6>><br>
 
                         <h6 class="quick_link_heading">WETLAB</h6>><br>
 
                         <div class="d-flex flex-column">
 
                         <div class="d-flex flex-column">
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/ProofOfConcept">PROOF OF CONCEPT</a>
+
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Proof_Of_Concept">PROOF OF CONCEPT</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Design">BLUEPRINT</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Design">BLUEPRINT</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Parts">PARTS</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Parts">PARTS</a>
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                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Education">EDUCATION</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Education">EDUCATION</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Communication">COMMUNICATION</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Communication">COMMUNICATION</a>
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Inclusivity">INCLUSION</a>
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                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Inclusivity">INCLUSIVITY</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Sustainable">SUSTAINABLE DEVELOPMENT</a>
 
                             <a class="quick_link" href="https://2021.igem.org/Team:IISER-Tirupati_India/Sustainable">SUSTAINABLE DEVELOPMENT</a>
 
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                         </div>

Revision as of 12:21, 15 October 2021


Ovi-Cloak

SCROLL

“Science is not finished unless it is communicated.”

Mark Walport

Introduction

Communicating science is as important as science as itself. It can help spark curiosity and build a scientifically literate community. Through our outreach activities, we wanted to connect with people from different backgrounds and levels of expertise in synthetic biology and foster discussion on science and synthetic biology. 

OUR GOALS

  • Help people appreciate the concept of synthetic biology and how it can be applied to solve local or global problems.
  • Understand the public perception of synthetic biology
  • Bridge the gender gap in STEM and encourage girl students to pursue higher education in the STEM field
  • Promote responsible research among the scientific community
  • Encourage students to learn about synthetic biology and pursue synthetic biology for their higher education.
  • Encourage synthetic biology enthusiasts to participate in iGEM and develop innovative solutions to different challenges.
  • Communicate our idea to the public.

Over the course of iGEM we engaged in various outreach activities to realise our goals and create a positive impact on the community. 

HOW DID OUR WORK IMPACT THE SOCIETY? BOX 

  • We inspired high school girls to develop innovative solutions to real-world problems using synthetic biology and encouraged them to pursue STEM for higher education through the “Gene Gala”.
  • We facilitated discussions on synthetic biology among students through “Gene ala”, a session with students from Agastya Foundation, and a Guest Talk at Synteraction.
  • We launched SynTrack, a one-of-a-kind podcast for beginners in synthetic biology discussing a wide range of topics to give them a holistic view of the subject.
  • We encouraged students to develop innovative solutions using synthetic biology and participate in iGEM through knowledge hub sessions, interacting with synthetic biology enthusiasts from our Institute via our guest talk at Synteraction.
  • We drafted a Handbook on Biotechnology Laws of India for Researchers and future iGEMers which can be used as a quick guide to go through relevant laws in a language easily understood by a person who is not from a legal background.
  • We conducted a survey to understand the perception of synthetic biology and GMOs worldwide in collaboration with Team MSP-Maastricht, Team Aachen, and Team IISER Pune.

While engaging with people from different walks of life and different perspectives, we learned to view synthetic biology in a different light, encouraging us to look at the bigger picture than as an isolated subject.

Gene Gala: Mini-Summer School

12th -16th July 2021

Science, Technology, Engineering, and Math (STEM) fields across the globe are highly dominated by men, with women making only a small fraction of the workforce in STEM. The lack of representation of women in science and social conditioning often leads them to underestimate their aptitude and steer them away from STEM. Our team wanted to take the initiative to bridge this gender gap in STEM, and this led us to launch “Gene Gala”, a Mini- Summer School to introduce high school girls to Synthetic Biology and encourage them to pursue higher education in the STEM field. To actualise this idea, we collaborated with Team IISER Kolkata and the Directorate of Education, GNCT Delhi, to conduct the Mini-summer school over the course of 5 days. Gene Gala saw the registration of over 90 girl students studying in their final year of high school.

Through Gene Gala, we wanted to provide a platform for girl students to cultivate a creative and problem-solving mindset and appreciate the concepts of Synthetic Biology. We utilised the initial four days to discuss the core concepts of synthetic biology, encourage them to participate in discussions, and communicate their ideas with their peers. 

We discussed biomolecules that make up a cell, bacterial genetics and how they are modulated, molecular techniques for redesigning genetic circuits and ethical concerns associated with synthetic biology. After each session, we divided the students into breakout rooms where we conducted fun quizzes, activities, and puzzles to keep them engaged and promote discussions. On the final day of the workshop, we introduced them to some exciting iGEM projects and encouraged them to develop solutions of their own to real-world challenges using the recently learned concepts of Synthetic Biology. The students actively contributed to the discussions and came up with several interesting ideas for designing their own Genetically Modified Organism.

When conducting Gene Gala, we were met with the challenge of covering broad concepts in a short duration of time and facilitating discussions. We came up with an effective break-up of the curriculum to achieve this and conducted breakout sessions for discussions and activities.

We encouraged them further to pursue STEM for higher education and provided the details on opportunities of Higher education in STEM in India, relevant scholarships, and the career paths in STEM through a day-wise Handbook which was provided to the students. We received very positive feedback from the students who participated in the Mini-summer school for inspiring them and giving them a sneak-peak into the world of Synbio and its opportunities.

We have documented our experience in conducting Gene Gala and have elaborately detailed our strategies for designing the curriculum, conducting the activity session and designing the materials used, which can be used by future iGEM teams when conducting similar workshops.

Click here on button to download the PDF file.

Download
Trulli
Trulli

Resources and Educational Tools

  1. Lesson Plan
  2. Class Material
  3. Handbook
  4. Gene Gala

See Contributions page for the Resources and Educational tools. 

SynTrack: The Podcast Series

We are a diverse team with people from different disciplines united by our shared passion for biology, and we were thrilled to share our excitement and curiosity about synthetic biology with people outside of our iGEM Team. This shared interest led us to launch SynTrack, a one-of-a-kind podcast launched for science enthusiasts who have just begun to explore the realms of Synbio. The podcast tries to capture the different flavours of the Synbio, ranging from experiments in the laboratory to opportunities in entrepreneurship, potential applications and ethical and moral implications of using genetic engineering. The podcast follows a casual conversational style podcast guiding the listeners through various aspects of Synbio as they get on with their everyday tasks. SynTrack provides an opportunity for the listeners to look at synthetic biology in a positive light and appreciate the potential of Synthetic biology as a powerful tool capable of revolutionising the landscapes of Health, Agriculture, Environment, and Bioenergy. The podcast included episodes that were recorded in collaboration with Spoorthy Gowda (Student at IISER Tirupati), Team Groningen, Team FCB- UANL, Team Ioannina, and TU Delft. The podcast was recorded over Zoom or Gmeet and launched on Spotify and Google Podcast.

The podcast was also translated into Hindi, a language widely spoken in India, and made available on Spotify. The podcast is beneficial for the local population who have better command over Hindi than English. Hindi is not a popularly used language for science communication. Launching the translated version of the podcast allowed us to tap into a broad audience of listeners and provided us with an opportunity to connect with listeners and communicate science crossing the language barriers.

Trulli

Resources

Let’s talk about iGEM: A Knowledge Hub session with Next Gen Scientists Foundation 

29 Aug 2021

iGEM has contributed significantly to foster innovation in synthetic biology among the student community, and the number of iGEM has increased over the years. However, we noticed that the number of teams participating from Asia in the competition is significantly lower when compared to its western counterparts from Europe and North America. We wanted to encourage students in India to participate in iGEM, which led us to collaborate with the Next Gen Scientists Foundation to launch an initiative, “Let’s talk about iGEM”, to reach out to other STEM students and Synthetic biology enthusiasts across the nation. 

The Next Gen Scientists Foundation is an organisation governed by a student body from students across India to connect all Indian Life Science Researchers. In our collaboration with them, our team hosted a Knowledge Hub session via their discord server called the Indian Life Science Network. This session connected us to students across the nation with interests in Synthetic Biology. The session was further made available via Youtube to make it more accessible to a larger audience. We invited Ms Shruti Shridhar, iGEM Ambassador Asia, as the speaker for the event to talk about the competition. Through the Knowledge Hub session, Shruti introduced the audience to the iGEM competition and discussed the values of iGEM. The session was conducted as a conversation between an iGEM ambassador and one of our team members like a live podcast, providing the audience with the provision to ask their questions and get them answered then and there.

The session guided the audience on what iGEM is, the multidisciplinary nature of the iGEM projects, and how iGEM fosters advancements in synthetic biology. It further navigated the audience through the process of iGEM, covering various aspects of the projects from Team Building, Funding, Collaborations, Human Practices, and the time frame of the competition. Ms Shruti also talked about the challenges faced by iGEM teams during the pandemic and how to manage and tackle them. She also encouraged the audience to avail the iGEM mentorship programme, which allows the iGEM team to connect with mentors to guide them on their iGEM journey. The session gave all-around information to Synthetic Biology Enthusiasts about the iGEM competition and how to navigate through the iGEM journey.

Insert Image of the poster

Resources

The Handbook of Biotechnology laws in India 

When working on the project, we realised the importance of biosafety when designing and performing experiments. This motivated us to look at the legal framework regulating biotechnology research in India. In this process, we realised that the biotechnology laws are scattered throughout The Constitution of India, which makes it difficult for people without legal knowledge to understand the legal protocols and refer to relevant laws. This inspired us to draft a handbook to guide the researchers through the relevant laws in Biotechnology that they might have to go through before their research. The book compiles relevant biotechnology laws in India and Multilateral legal agreements of which India is a part of. It attempts to capture the multiple facets of law that must be abided by while performing scientific experiments in biotechnology in one place. When reading the various biotechnology laws, we also recognised some gaps in the legal framework, which motivated us to incorporate a section on gaps and unattended problems in the legal architecture into the Handbook. We also added a section for future iGEM teams to refer to with regard to safety practices that must be followed over the course of their project. The book was launched with the aim to promote responsible research among the research community in India. 

In collaboration with Team IISER Thiruvananthapuram, our team identified important biotechnology laws, identified the relevant Biotechnology laws, and designed the framework of the Handbook. The book is subdivided into four chapters:

  • Animal experimentation and Clinical trials
  • Export-Import Policies
  • Environmental Safety
  • Biocontainment and Biowarfare

During the drafting of the Handbook, we met with Dr Harilal Madhavan, a development economist and faculty at IISER Thiruvananthapuram; Miss Parvathy, a student from the National Law School of India University; and Dr Subha C C, a faculty at the Department of School of Law in Christ University, Bangalore who provided us valuable advise and feedback enabling us to revise and improve our Handbook.

Resources

The Handbook of Biotechnology laws in India.

Interactive Workshop with Agastya Foundation 

When collaborating with iGEM Teams and reading the past iGEM projects, we were deeply awed by the possibilities of synthetic biology and its potential. This motivated us to reach out to students to introduce them to synthetic biology and encourage them to pursue Synbio for higher education. To realise this, we conducted a workshop collaborating with Agastya Foundation for students with a passion for Biology.

Agastya Foundation is a non-profit organisation based in Bangalore, India, aiming to nurture creativity and curiosity through education to students from lower economic strata. During the session, we introduced them to the foundational concepts in synthetic biology and gave them a flavour of the multidisciplinary facets of synthetic biology. We further introduced them to iGEM to inspire them to participate in iGEM in future. Following this, we conducted a brainstorming session, during which we encouraged them to develop ideas based on synthetic biology for contraception. The students actively participated in the discussions and came up with creative solutions and questions about developing novel contraceptives. We further discussed with them the opportunities of STEM education in India and the relevant scholarships and internship opportunities to pursue STEM. We also introduced them to Ovicloak, discussing the biological concept behind the idea, our motivation and plans for implementation. 

Trulli

International Survey on Genetically Modified Organisms

Our team conducted an International survey in collaboration with Team MSP- Maastricht, Team Aachen, and Team IISER-Pune-India to understand the attitude of the public towards genetic engineering organisms and the applications of synthetic biology across the world. The survey received 259 responses from Asia and Europe and was conducted through Google Forms. 

The survey questions were designed to understand the perception towards synthetic biology and GMOs, how receptive are people towards the different applications of synthetic biology and understand the concerns people have about GMOs. The survey revealed that the people largely had a positive perception towards synthetic biology and GMOs while they also expressed their concerns about potential damage to the environment, potential harm to human health, potential misuse, and ethical concerns on the creation of artificial life. We also observed that the opinion towards GMOs varied depending on the context of the application. Our analysis also revealed that the general outlook towards synthetic biology and concerns towards its usage were strikingly similar in both Europe and Asia.

The results of the survey analysis are published on the iGEM wiki page, which would be useful for future iGEM teams to gain an insight into how receptive people will be towards synthetic biology and its various applications.

Resources

International survey on GMO

An Interview with iGEM Ambassador Asia

One of our team members interviewed the iGEM Ambassador of Asia, Miss Shruti Shridhar, to write an article to be published in Next Gen Scientists Foundation (NGSF) Blog. NGSF is a community of life science researchers and students predominantly in India. During the interview conducted we discussed her iGEM experience, the journey to becoming the iGEM Ambassador to Asia, plans on expanding iGEM in India, and the challenges in introducing iGEM to Indian colleges and highschool. 

While a large number of resources are available on the iGEM journey, only very few resources are available to know about opportunities and experience after iGEM, which motivated us to interview Miss Shruthi and publish a blog article.

The Interview article would be helpful for current and future iGEMers to learn about opportunities after-iGEM. The article is currently in the process of being published in the NGSF Blog, which has viewers from over 73 different countries allowing us to reach out to students of the scientific community.

Guest Talk at Synteraction

21 July 2021

Our team was invited to deliver a guest talk at Synteraction, a summer school programme launched by Team iGEM IIT Madras for their first year B.Tech students. Through the talk we wanted the audience to appreciate how synthetic biology can be employed to solve social challenges and how various disciplines intersect and overlap during the development of a project. During the talk, we introduced the audience to our project idea: Ovicloak - A Novel Contraceptive. We introduced the audience to the biological concepts on which the idea is based. We discussed with the audience about the engineering aspects of the idea and logic behind the genetic engineering circuits employed. We also discussed the importance of the kill switch in ensuring the biosafety aspect of our project and the mechanism behind the working of the kill switch. We discussed the multidisciplinary nature of the project and introduced the audience to the various facets of our project, such as genetic engineering, mathematical modelling, and human practices, to help them appreciate how the various disciplines of physics, mathematics, biology, and social science is integrated during the development of the project. We walked them through the importance of Human Practices of iGEM and how our team incorporated the iGEM values into our project. We explained how we engaged with the stakeholders and incorporated their feedback while developing the project. We further discussed the outreach activities launched by our team to engage with the public and their importance in initiating thoughtful discussions about the values related to our project. We also navigated the audience through our iGEM journey and how we tackled the challenges posed by the pandemic. 

Trulli

A session with Synbio enthusiasts 

Our team held an interactive session with the Synbio enthusiasts in our Institute to kickstart their iGEM journey. Through this session, we guided the potential future iGEMers from our Institute in planning and developing their project and addressed their queries regarding the various aspects of iGEM. During the interactive session, we walked them through our iGEM journey from team building to project development and the challenges we faced during the pandemic.

We discussed the multidisciplinary nature of the project and building a diverse team with different fields of expertise to help look at the problem from different perspectives. We also discussed the timeline of the iGEM Project to help them plan their iGEM journey. The importance of identifying the stakeholders and engaging with them to integrate their feedbacks while developing the project was also discussed, and some initial guidelines for the funding aspect of the project were also provided.

Publishing an article in the Manthan magazine 

Our team published an article in the Manthan magazine, a student magazine launched by the students of the Indian Institute of Science Education and Research, Mohali, to promote our project Ovicloak among the student community. Through the article, we wanted to provide an overview of our project to the student community across India. We used this opportunity to discuss the goals envisioned by our team, the hurdles we faced during its development, the progress we made, and the impact our project could have on the world around us. We communicated to the readers how Ovicloak has the potential to address issues related to reproductive health, family planning, inclusivity, and ecological crises on both the global and local scales. We also emphasised how Ovicloak was designed to make the contraceptive inclusive for all uterus owners as compared to alternative options available in the market which are not inclusive for individuals with different hormonal levels, reproductive tract disorders, fertile intersex and trans individuals. We also discussed through the article how Ovicloak could be a safer alternative with respect to the health of contraceptive users and the environment at large. 

Resources

Manthan article

DNA Day with MolecularCloud

The Discovery of DNA is an important milestone in the history of genetic research. Elucidation of the structure of the DNA by James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin and colleagues led to the subsequent development in molecular biology and genetic engineering. To celebrate and commemorate this success, three of our team members created an artistic representation of DNA. 

Our team member, Shivam Kumar, designed a pencil sketch titled “ASTRA DNA”, which represents an amalgamation of biology and astronomy depicting the advancements and developments made in the macro and micro world. The second artwork was created by Lochan Chaudhari titled “DNA dancing to the tune of life”, which represents the dancing DNA being transcribed to give rise to the melody of life. Komal Pati created the third artwork titled “The secret of life”, illustrating how DNA lays the foundation of life, leading to the diverse creatures we see today. These images were published by MolecularCloud on their website for the DNA Day Photo Contest of which ASTRA DNA and DNA dancing to the tune of life received Silver and Bronze prize and The secret of life received an honourable mention. The images were also published in our social media handle to communicate to a broader audience.

Trulli

Engaging with the public through social media 

Our team engaged with the public through its social media handles, giving a sneak peek of our project work from time to time. To provide the audience with a short glimpse into our daily routine at the laboratory, we conducted Insta live demonstrating Agarose gel preparation and SDS gel preparation. To introduce our viewers to our idea- Ovicloak, we released our promotional video to communicate to a large audience about our project idea and how Ovicloak can be a safe and sustainable alternative to the currently available contraceptives. We subsequently also released the promotional video in Sign language to communicate to a more inclusive audience. 

Resources

Instagram

Twitter

Interview with Genscript

Our team was given an opportunity to be interviewed by Genscript to discuss Ovicloak and how we used synthetic biology to develop a contraceptive. The interview provided us with an opportunity to interact with professionals in the field of synthetic biology and gain public exposure. During the interview, we discussed our idea and the social impact of the project. We also discussed how we plan to incorporate Human Practices into our project. We also discussed our aim to instigate a fresh perspective on contraceptive development using synthetic biology. The recorded video of the session was uploaded on Youtube by Genscript, which provided us with an opportunity to communicate our idea to the scientific community. 

Genscript Interview
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